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InvisibleVal
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
Re: First time trying out Agar this weekend - A couple general questions [Re: twistedty]
    #21324001 - 02/24/15 05:49 PM (9 years, 10 months ago)

Quote:

twistedty said:
i just waited until i knew there was no more pressure in pc, grab the bottle with an oven mitt and place in sab.  let cool down until i could handle it with my hands and pour my dishes.




This is a good method for SAB (IMO) but I have a Hood so i have no expertise there.

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OfflineNos-hroom
Oneironaut's Apprentice
Male


Registered: 08/13/14
Posts: 183
Last seen: 9 years, 6 months
Re: First time trying out Agar this weekend - A couple general questions [Re: Val]
    #21324047 - 02/24/15 05:56 PM (9 years, 10 months ago)

You know ... that's a good fucking point. I'm still stuck in the SAB mindset.

Changing things up, as long as I remember to grab it with a mitt. :congrats:

Edit: How accurate has your IR therm been for you? I've been wanting to pick one of those up for cooking alone, but if it doubles as good for mycology, then it's on the list for real, this time.

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InvisibleVal
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
Re: First time trying out Agar this weekend - A couple general questions [Re: thisisfielding]
    #21324074 - 02/24/15 05:59 PM (9 years, 10 months ago)

Quote:

thisisfielding said:
So PC mixture > Let Cool > Pour Agar - How long does it take for the agar to set? Should I be able to inoculate shortly after pouring or should I just pour them all, put them back into sleeve, close sleeve up and put in fridge. My plan is to make 500ml of mixture, 2 x 10 dish sleeves,.. use the first 2 in each sleeve (four total) to drop a couple drops of MS syringe solution on. Parafilm them all then see what happens in the next couple days. Should I store them all in the lunch box fridge method or does that completely stall growth on the dishes? Where do you guys usually place the sleeves as they are cooling then after they've been inoculated?




I would avoid the fridge...unless your doing a cool weather strain. I usually pour, let cool for an hour, then do your work on them. I wrap them with para-film and place in a alcohol wiped Tupperware container w/lid. I then place in a appropriate temperature environment for that particular strain.

Edited by Val (02/24/15 06:00 PM)

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InvisibleVal
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
Re: First time trying out Agar this weekend - A couple general questions [Re: Nos-hroom]
    #21324108 - 02/24/15 06:03 PM (9 years, 10 months ago)

Quote:

Nos-hroom said:


Edit: How accurate has your IR therm been for you? I've been wanting to pick one of those up for cooking alone, but if it doubles as good for mycology, then it's on the list for real, this time.




Mine is pretty accurite...depends on the material your reading...you play with it a little bit, and you'll figure it out. There pretty cheap on amazon...and theres several of them.

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