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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf] 1
#21321215 - 02/24/15 05:25 AM (9 years, 10 months ago) |
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Quote:
THE_LOAF said: Nobody never gets contamination.
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tourrat
humanbeinganimal


Registered: 01/16/14 
Posts: 394
Last seen: 9 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: eatyualive]
#21321358 - 02/24/15 06:43 AM (9 years, 10 months ago) |
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Quote:
eatyualive said:
Quote:
THE_LOAF said: Nobody never gets contamination.
I spent about three months thinking I was a bad ass ....then got smacked so hard by the green monster I had to TOTALLY shut down....clean the hell out of everything ...ten times lol. That was last winter and I'm just now getting over it . Very humbling experience to say the least.
-------------------- Knee deep in the hotel tub.....
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 48 minutes
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
#21321385 - 02/24/15 06:53 AM (9 years, 10 months ago) |
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Indeed. Getting bitch-slapped, and boot-worked by contamination is a kind of unwritten rite-of-passage into this hobby.
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tourrat
humanbeinganimal


Registered: 01/16/14 
Posts: 394
Last seen: 9 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
#21321419 - 02/24/15 07:06 AM (9 years, 10 months ago) |
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Yep....I think it all started with one bad syringe....g2g ended up being about 50 jars and ten tubs all told....sucked bad! I went from hero to zero in about a week. lol.
-------------------- Knee deep in the hotel tub.....
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
#21321510 - 02/24/15 07:41 AM (9 years, 10 months ago) |
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Bacteria has been the worst for me. I can still remember losing at least 6 lbs of mushrooms b/c of it. Taught me to be super picky about spawn though.
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tourrat
humanbeinganimal


Registered: 01/16/14 
Posts: 394
Last seen: 9 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
#21321571 - 02/24/15 08:04 AM (9 years, 10 months ago) |
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Quote:
blackdust said: Bacteria has been the worst for me. I can still remember losing at least 6 lbs of mushrooms b/c of it. Taught me to be super picky about spawn though.

Yep...been there done that as well...I started pc'ing my grains longer,quit trying to incubate with a heating pad/towel contraption I had and made sure I had no water pooling at the bottom of my jars ( let my grain dry out overnight) poof no more bacteria.
-------------------- Knee deep in the hotel tub.....
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 48 minutes
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
#21321592 - 02/24/15 08:11 AM (9 years, 10 months ago) |
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Force-incubation in this hobby is a no good. Mycelium best incubates at the same temperature as humans, and is why "room-temperature" 70-75F) is prescribed to incubate mushroom mycelium @. Room temperature is just a grossly simplified way of saying incubate mushroom mycelium @ 70-75F. The same temperature range we incubate ourselves at.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
#21321637 - 02/24/15 08:24 AM (9 years, 10 months ago) |
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Grain prep can be tricky. I have the most experience with rye but just started using WBS and popcorn. Had some really good popcorn made the other day with the grain haul intact the after the PC cycle the fuckers exploded on me a bit. Must of hydrated them to much before the cycle to cause the hauls to burst. Remember, grain hauls are contam resistant and thats one of the reasons that burst grains are bad.

Ham, Some people have colonization and fruiting rooms in basements, by windows, or live in an older house that is not insulated well. The mean temperature of my grow room right now is 65F. 65F is not a good temperature for Psilocybe cubensis. The cold tempeature slows down colonization and the culture is not as vigorous and is more prone to stalling out, weak fruits, drying out, etc. Even though my fruiting room has a mean temp of 65F the majority of the house is at 73F. The grow room is closed off from the air vents and thus does not get warmed up like the rest of the rooms of the house. I was thinking of having a space heater by the door to keep the temps up to 70 - 75F (and it was working) but I had to move the space heater b/c my wife wanted it. I tried to run a second space heater for the room but my breaker can't handle the load and blows.
I think Kizzle was talking about passive incubation a few months ago. Suggested having spawn jars and bags to be sub,merged in vermiculite to help insulate the spawn. Spawn generates heat, so if the verm can keep the heat in them a desired temp could be reached. The time difference can be as large as 15 days in colonization times because of temp. This is just my experience.
I used to live in the Southbay of Cali and never had temp or RH issues since the weahter was the same everyday. About 70F with a nice ocean breeze as I lived within 1 mile of the ocean. Now with me living North East again and am dealing with a harsh winter while living in a trailer my growing skills are being tested a bit as this is all new to me. Rh is easy to take care of but keep the room warm enough is my challenge at the moment.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf] 1
#21321852 - 02/24/15 09:22 AM (9 years, 10 months ago) |
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Quote:
THE_LOAF said: Force-incubation in this hobby is a no good. Mycelium best incubates at the same temperature as humans, and is why "room-temperature" 70-75F) is prescribed to incubate mushroom mycelium @. Room temperature is just a grossly simplified way of saying incubate mushroom mycelium @ 70-75F. The same temperature range we incubate ourselves at.
This forum needs to be simplified. If we started using the term incubate for room temperature and forced-incubation for actually incubating inside of something, people would be confused as hell. You would see crazy TiT incubators everywhere. You're saying the same thing as everyone else but getting all butt hurt over terminology. Sure you can be technically correct and call a house an incubator, but jesus christ.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 48 minutes
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
#21321884 - 02/24/15 09:32 AM (9 years, 10 months ago) |
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I'm not butthurt at all.
and don't appreciate being falsely accused of that. Seems to me that the ones who are so quick to accuse others of being butthurt are the ones who are butthurt. Thank you for your concern though.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
#21321893 - 02/24/15 09:34 AM (9 years, 10 months ago) |
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Aight dude sorry then. I'll knock it off. I'm just trying to keep future noobs from roasting their jars.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
#21321945 - 02/24/15 09:50 AM (9 years, 10 months ago) |
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posts like these are confusing to me and probably a lot of newbs too:
Quote:
THE_LOAF said: When will you kids learn, room temperature is INCUBATION. 
Quote:
THE_LOAF said: NEVER INCUBATE IN THIS HOBBY 
where do you keep your jars then? if inside is incubating and incubating is bad? I might be nit picking now but I kinda agree with flabbergasted, it gets confusing
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
#21321957 - 02/24/15 09:51 AM (9 years, 10 months ago) |
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I think he was being facetious space.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 48 minutes
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
#21322085 - 02/24/15 10:27 AM (9 years, 10 months ago) |
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Correct. My spawn INCUBATES at 70-75F, and 50% RH inside of my makesshift, poorman's sterile laboratory.


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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
#21322134 - 02/24/15 10:40 AM (9 years, 10 months ago) |
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context. It's all about context.
-------------------- The Basics
A little civility goes a long way
The Noob Forum
The Hammock Hangers' Forum
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
#21322210 - 02/24/15 11:01 AM (9 years, 10 months ago) |
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Wow ,
You guys are really having a go.
Relax guys, my APE tub just triched out and I had other stuff trich out before.
Nobodies perfect lol. I don't think I'm a badass, I just think I can acquire and use information very well. Once again, for three months into this hobby and my first pin being less then 2, I'm doing GREAT. That's all that matters to me and that's why I can say my APE tub triched out (Because I used bacterial spawn on purpose from a dirty syringe) and take pictures and put it all over the internet because I don't believe in ego in failure.
I fucked up, learn from it, that's why I have a 50 page grow log of straight fuck ups, because people have read it and corrected themselves.
At the end of the day I feel like you older guys should stop trying to get all the praise and put the attention where it deserves, on new cultivators who are bringing this into the next generation. Work on your experiments, post your results with like-minds, write up teks and simplify the hobby for EVERYONE. Is that not the goal? Even you Blackdust, who seems hell bent on taking Ham's side because you're both part of the tribe. I understand cleanliness, I just don't need someone who's been doing this for YEARS to come down on me and go "BlahBlahBlah, proper lab technique, blahblahblah." when in all reality it's just a box with holes cut in it. I spend my time on the shroomery helping people, not arguing politics or semantics.
I grow drugs bro, and help other people grow them and I do a damn good job at both. Already 90+ 5 Shroom ratings?
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
Edited by taGyo (02/24/15 11:08 AM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
#21322266 - 02/24/15 11:13 AM (9 years, 10 months ago) |
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I've been doing this seriously since about August, so 6 months. I've had every contam in the book, but bacteria has been my biggest headache. I've only lost 2 tubs to trich and one to mucor. But I agree. Everyone will have to battle contams at some point.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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tourrat
humanbeinganimal


Registered: 01/16/14 
Posts: 394
Last seen: 9 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
#21322482 - 02/24/15 12:11 PM (9 years, 10 months ago) |
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This arguement about incubating is just plain stupid. HAM I get where you're coming from but god damn dude you're doing nothing but confusing the hell out of people for the sake of some stupid terminology beef. Room temp is a whole different thing than incubation when used in the context were using it in.
The reason we don't incubate in an incubator is because if you use an incubator you end up heating the bottom of the jar slightly more than the rest of the jar which causes condensation....which in turn drips to the bottom of the jar where it becomes the perfect breeding ground for bacteria which favors the extra warmth your incubator is so conveniently creating.
This doesn't happen nearly as bad when just left at "room temp" because the jat is warm evenly from all directions.
I realize 90% of us know this but for the few that don't this small tied bit of info can save a lot of headache.
-------------------- Knee deep in the hotel tub.....
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 48 minutes
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
#21322600 - 02/24/15 12:44 PM (9 years, 10 months ago) |
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Sorry for your confusion, but I am going to continue to use the term incubation correctly in hopes of clearing up any confusion. It is this cultivators opinion that the falling into line of it's meaning will piggy-back on my efforts. Thank you for your concern though.
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tourrat
humanbeinganimal


Registered: 01/16/14 
Posts: 394
Last seen: 9 years, 4 months
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
#21322663 - 02/24/15 01:01 PM (9 years, 10 months ago) |
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This begs the question....my house isn't heated at all right now...its simply at ambient temperature... you're claiming that's incubating?...just to clarify.
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