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OfflineAlwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
PC times
    #21321066 - 02/24/15 03:27 AM (9 years, 10 months ago)

I may have screwed up.  On my first mini monos 1 of 3 trich'ed out before it was 100% colonized.

I decided to increase my PC times. I was doing 60 min for cakes and 105 for grains. I increased it to 120 for grains and 75 for cakes.

Well I PCed 12 half pint brf cakes last night for 75 minutes. When I inoculated them this morning, I noticed that the brf/verm mixture under the dry verm layer had shrunk substantially, pulling away from the sides. The micropore tape on top was soaked.

I assume the extra 15 minutes screwed up my water content.

Will this effect colonization? Did this mess up the dry verm filter? I can see where some dry verm trickled down where the cake pulled away from the side. Also the lost moisture had to pass through the dry verm layer, so is it dry anymore?

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OfflineSharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia Flag
Last seen: 8 years, 6 months
Re: PC times [Re: Alwaysanoob]
    #21321090 - 02/24/15 03:58 AM (9 years, 10 months ago)

Are you letting the PC cool down over night?

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OfflinetheBitterBuffalo00
Seeking


Registered: 01/27/15
Posts: 109
Last seen: 9 years, 9 months
Re: PC times [Re: Sharpstuff]
    #21321110 - 02/24/15 04:09 AM (9 years, 10 months ago)

I was getting bad trich on my wbs so i did an experiment and didn't knock up some jars on my last run. I just shook them and let em sit. They never contaminated while the ones i inoculated with colonized agar did. It was my sterile procedure.


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OfflineStraya
Wot?!


Registered: 03/04/12
Posts: 705
Loc: Flag
Last seen: 5 years, 8 months
Re: PC times [Re: theBitterBuffalo00]
    #21321133 - 02/24/15 04:28 AM (9 years, 10 months ago)

following

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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: PC times [Re: Straya]
    #21321144 - 02/24/15 04:37 AM (9 years, 10 months ago)

Bacteria would be your culprit if it were your pc times not trich. Mold contamination comes from improper sterile technique or from contaminated cultures.

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OfflineAlwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
Re: PC times [Re: insanemike]
    #21321229 - 02/24/15 05:34 AM (9 years, 10 months ago)

I let the PC cool overnight. I turned off the stove right before bed and opened it up approximately 7 hours later.

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OfflinehamloafM
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Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 20 hours, 25 minutes
Trusted Cultivator
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Re: PC times [Re: theBitterBuffalo00]
    #21321290 - 02/24/15 06:10 AM (9 years, 10 months ago)

Quote:

theBitterBuffalo00 said:
I was getting bad trich on my wbs so i did an experiment and didn't knock up some jars on my last run. I just shook them and let em sit. They never contaminated while the ones i inoculated with colonized agar did. It was my sterile procedure.



Correct.  Nice experiment.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflinehamloafM
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Re: PC times [Re: Alwaysanoob]
    #21321307 - 02/24/15 06:17 AM (9 years, 10 months ago)

Quote:

Alwaysanoob said:
I may have screwed up.  On my first mini monos 1 of 3 trich'ed out before it was 100% colonized.

I decided to increase my PC times. I was doing 60 min for cakes and 105 for grains. I increased it to 120 for grains and 75 for cakes.

Well I PCed 12 half pint brf cakes last night for 75 minutes. When I inoculated them this morning, I noticed that the brf/verm mixture under the dry verm layer had shrunk substantially, pulling away from the sides. The micropore tape on top was soaked.

I assume the extra 15 minutes screwed up my water content.

Will this effect colonization? Did this mess up the dry verm filter? I can see where some dry verm trickled down where the cake pulled away from the side. Also the lost moisture had to pass through the dry verm layer, so is it dry anymore?



Sounds like you mabye forgot to put foil over your media closures before PC'ing.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineAlwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
Re: PC times [Re: hamloaf]
    #21321356 - 02/24/15 06:42 AM (9 years, 10 months ago)

I did cover with foil and I tried to make sure it was form fitting, rubbing it down well. my bottoms of the lower layer of jars was at least 2 inches above the water.  For PF Tek I use 2 jar racks below a trivet.

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OfflinehamloafM
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Re: PC times [Re: Alwaysanoob]
    #21321377 - 02/24/15 06:49 AM (9 years, 10 months ago)

Very well then, but 2 inches of water is too much.  The water boils up into the media vessels if you pressure sterilize them submerged into that much water.

You only need to fill the PC up with water until the water level comes just above the insert of the PC, and just touches the bottom of the media vessels.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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