|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Alwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
|
PC times
#21321066 - 02/24/15 03:27 AM (9 years, 10 months ago) |
|
|
I may have screwed up. On my first mini monos 1 of 3 trich'ed out before it was 100% colonized.
I decided to increase my PC times. I was doing 60 min for cakes and 105 for grains. I increased it to 120 for grains and 75 for cakes.
Well I PCed 12 half pint brf cakes last night for 75 minutes. When I inoculated them this morning, I noticed that the brf/verm mixture under the dry verm layer had shrunk substantially, pulling away from the sides. The micropore tape on top was soaked.
I assume the extra 15 minutes screwed up my water content.
Will this effect colonization? Did this mess up the dry verm filter? I can see where some dry verm trickled down where the cake pulled away from the side. Also the lost moisture had to pass through the dry verm layer, so is it dry anymore?
|
Sharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 8 years, 6 months
|
|
Are you letting the PC cool down over night?
|
theBitterBuffalo00
Seeking


Registered: 01/27/15
Posts: 109
Last seen: 9 years, 9 months
|
|
I was getting bad trich on my wbs so i did an experiment and didn't knock up some jars on my last run. I just shook them and let em sit. They never contaminated while the ones i inoculated with colonized agar did. It was my sterile procedure.
|
Straya
Wot?!


Registered: 03/04/12
Posts: 705
Loc:
Last seen: 5 years, 8 months
|
|
following
|
insanemike

Registered: 02/23/14
Posts: 4,272
|
Re: PC times [Re: Straya]
#21321144 - 02/24/15 04:37 AM (9 years, 10 months ago) |
|
|
Bacteria would be your culprit if it were your pc times not trich. Mold contamination comes from improper sterile technique or from contaminated cultures.
|
Alwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
|
|
I let the PC cool overnight. I turned off the stove right before bed and opened it up approximately 7 hours later.
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 44 minutes
|
|
Quote:
theBitterBuffalo00 said: I was getting bad trich on my wbs so i did an experiment and didn't knock up some jars on my last run. I just shook them and let em sit. They never contaminated while the ones i inoculated with colonized agar did. It was my sterile procedure.
Correct. Nice experiment.
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 44 minutes
|
|
Quote:
Alwaysanoob said: I may have screwed up. On my first mini monos 1 of 3 trich'ed out before it was 100% colonized.
I decided to increase my PC times. I was doing 60 min for cakes and 105 for grains. I increased it to 120 for grains and 75 for cakes.
Well I PCed 12 half pint brf cakes last night for 75 minutes. When I inoculated them this morning, I noticed that the brf/verm mixture under the dry verm layer had shrunk substantially, pulling away from the sides. The micropore tape on top was soaked.
I assume the extra 15 minutes screwed up my water content.
Will this effect colonization? Did this mess up the dry verm filter? I can see where some dry verm trickled down where the cake pulled away from the side. Also the lost moisture had to pass through the dry verm layer, so is it dry anymore?
Sounds like you mabye forgot to put foil over your media closures before PC'ing.
|
Alwaysanoob
Noob
Registered: 11/23/14
Posts: 133
Last seen: 9 years, 8 months
|
Re: PC times [Re: hamloaf]
#21321356 - 02/24/15 06:42 AM (9 years, 10 months ago) |
|
|
I did cover with foil and I tried to make sure it was form fitting, rubbing it down well. my bottoms of the lower layer of jars was at least 2 inches above the water. For PF Tek I use 2 jar racks below a trivet.
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 21 hours, 44 minutes
|
|
Very well then, but 2 inches of water is too much. The water boils up into the media vessels if you pressure sterilize them submerged into that much water.
You only need to fill the PC up with water until the water level comes just above the insert of the PC, and just touches the bottom of the media vessels.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 495 topic views. 28 members, 160 guests and 97 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|