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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21312572 - 02/22/15 11:29 AM (9 years, 10 months ago)

Oh,

No problem I'm setting my SAB up tonight actually (If I find the motivation 'cause lord knows that's a problem). I'll wipe it down real good and shit and do two things:

Drag a plate through the air and open a plate and set it off to the side while I do my work.

If both don't contam then shout out to my SAB but I'm gonna guess the one I drag may not contam but the one I leave to the side will.

I'll keep this thread updated.


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Dominus fortunae meae sum

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OfflinehamloafM
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21312614 - 02/22/15 11:39 AM (9 years, 10 months ago)

If I were still inside of an SAB today, I would plastic off a small section of an area, and set up my SAB inside of a sterile lab environment, using the same procedures to enter the lab as I do with my flowhood. 

There is such a thing as Still Air Laboratories & Still Air Laboratory Techniques and Procedures.  :thumbup:


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21313265 - 02/22/15 01:52 PM (9 years, 10 months ago)

Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:

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OfflinecronicrMFacebook
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21313281 - 02/22/15 01:56 PM (9 years, 10 months ago)

:popcorn:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleMrGiraffe

Registered: 04/04/14
Posts: 3,149
Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21314337 - 02/22/15 06:34 PM (9 years, 10 months ago)

Quote:

blackdust said:
Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:




I once had a poured plate pop up with a single spot of bacteria, since it was wasted I decided to take it into my basement greenhouse and wave that bitch around just to see what kind of stuff I'd pick up.  That plate sat around for like 5 days with only the first spot of bacteria still on it.  I opened it up again in my upstairs kitchen/living room and got mold within 3 days.  I know there's shit growing in my basement.  It would grow on the extension cords and stuff when I was running the coolmist 24/7.  Best I can figure is the coolmist running 24/7 keeping the air at 99% and the walls of the greenhouse saturated maybe collected the spores and kept them from floating around to much :shrug:.  Not the most scientific thing, but I was still surprised the plate didn't get covered until I opened it upstairs.


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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: MrGiraffe]
    #21314366 - 02/22/15 06:39 PM (9 years, 10 months ago)

I recconmend any doing work in a still air room or a SAB to spray either

A. water around the work area to catch the molds to be wiped away

or

B. disinfectant spray


I always used disinfectant spray


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InvisibleNumeroEno
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21314623 - 02/22/15 07:35 PM (9 years, 10 months ago)

I spray my SAB with a mix of water, dish soap, rinse aid, and bleach. I was using lysol before, and now I get much fewer contams. The rinse aid just helps me see better. Lysol wipes are the shit though.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316145 - 02/23/15 06:27 AM (9 years, 10 months ago)

Quote:

NumeroEno said:
I spray my SAB with a mix of water, dish soap, rinse aid, and bleach. I was using lysol before, and now I get much fewer contams. The rinse aid just helps me see better. Lysol wipes are the shit though.



:sunspots:

Iso and water for me. Paper towel to white it down.

Quote:

blackdust said:
Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:




It's an experiment to show you you're not in control of everything around you. To be honest I did a very quick open-air agar syringe drop and I saw no contams lol.

I don't think anyone is ever saying it's impossible, but for consistent results people prefer an SAB.


--------------------
Gyo's Better Grows
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Dominus fortunae meae sum

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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21316272 - 02/23/15 07:22 AM (9 years, 10 months ago)

The reason I like the disinfectant spray is that the spray is finer and thus goes through the air better the catch more debris. I'm sure they have spray bottles that can do this to though. I wipe everything down with iso.


===
Gy0, you would be happy to know that my still air room has become overwhelmed with fruiting chambers and I was forced to pull out my SAB to do work in my bedroom. I didn't have any problems using the SAB but prefer the still air room. Had to clean it out good since I was fruiting in it. :rofl:


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Offlinespacechildo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316277 - 02/23/15 07:24 AM (9 years, 10 months ago)

how do you keep the dirtiest thing in your house out of the SA-room and still be able to do work in it?
do you have a glory hole in your wall? :tongue2:

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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21316331 - 02/23/15 07:50 AM (9 years, 10 months ago)

1. shower / brush teeth
2. make sure all fans are off for an 1 hr before work
3. gloves / face mask
4. Spray room and wait 5 min
5. Wipe everything with iso / use torch lighter
6. make use of SHIPs and directly injecting bags
7. Know in advance what will be done and work fast
8. cause as little air movement as possible


**I have also always ran a air purifier in the bedroom. The still air room has always been a closet for me. Not sure if the air purifier has any effect. I seen still air rooms set up many different ways.

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316342 - 02/23/15 07:53 AM (9 years, 10 months ago)

I'm impartial to any method. Part of the whole philosopher thing, I do what works. I just know that you're doing things with prior experience which vastly outweighs noobiness.

Your methods aren't really out there so much as we just don't suggest them to new members,

:shrug:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum

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Offlinespacechildo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316348 - 02/23/15 07:56 AM (9 years, 10 months ago)

ships only? so you dont do agar in the room then?
I still prefer a box so I can keep myself out of the SA environment,
no matter how many showers you take you are still the dirtiest thing in your house :tongue2:
not just you, I am as well, not meant as an insult!

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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21316433 - 02/23/15 08:29 AM (9 years, 10 months ago)

I know space. I have done a few dozen agar plates in a still air room. Here is my old agar bottle. Never got to much into it as I diddnt really need it for cubes.




I did just make some pasty plates a few days ago in my SAB though. I like this new no-pour agar. Im getting my skills with agar and cubes so when I get some more exotic species I will have the confidence to do what I want. The main species I am working towards at the moment is

Psilocybe cyanofriscosa
Psilocybe Subaeruginosa
Panaeolus (Copelandia)

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InvisibleNumeroEno
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316589 - 02/23/15 09:09 AM (9 years, 10 months ago)

BD, I'll hook you up with some cyanofriscosa/allenii if I can get my print to germinate. I'll send you what's left of the print after I get it going. It is a wild print so you'll need either antibiotic agar or extreme luck to get it going. I can also send you 50mgs of gentamycin sulfate, which is enough for a liter of agar.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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OfflinehamloafM
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21317572 - 02/23/15 12:49 PM (9 years, 10 months ago)

Quote:

spacechildo said:
ships only? so you dont do agar in the room then?
I still prefer a box so I can keep myself out of the SA environment,
no matter how many showers you take you are still the dirtiest thing in your house :tongue2:
not just you, I am as well, not meant as an insult!



Your trash can is a FUCK ALL of a breeding ground for contamination.  After that, pets (if you have any).


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How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21317576 - 02/23/15 12:50 PM (9 years, 10 months ago)

Yeah but no one is cultivating in a trash can or around one. I think he meant dirtiest thing you SHOULD be introducing to a Still-Air environment lol.


--------------------
Gyo's Better Grows
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Dominus fortunae meae sum

Edited by taGyo (02/23/15 12:51 PM)

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OfflinecronicrMFacebook
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21317587 - 02/23/15 12:52 PM (9 years, 10 months ago)

Quote:

taGyo said:
Yeah but no one is cultivating in a trash can Ham.



:facepalm:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: cronicr]
    #21317590 - 02/23/15 12:52 PM (9 years, 10 months ago)

:laugh2:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: cronicr]
    #21317597 - 02/23/15 12:53 PM (9 years, 10 months ago)

:notamused:

Really Cron? :lmafo: Okay, try not taking out the trash for a few days, then removing the plastic liner and fruiting in that.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum

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