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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 8 years, 10 months
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Alternatives for long term myc storage?
#21313680 - 02/22/15 03:59 PM (9 years, 10 months ago) |
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Are there any? I don't have vials or vacu-tainers. Any grocery store teks for isolate storage?
Thanks in advance.
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,313
Last seen: 5 months, 30 days
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Re: Alternatives for long term myc storage? [Re: Mushroom_J]
#21313700 - 02/22/15 04:07 PM (9 years, 10 months ago) |
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Baby food bottle agar, They are the tiniest jars you can find as cheap as .40 cents each. Petris store well, lc`s store well, Umm Master grains jars store well.. The ideal storage temperature for most strains of spawn is 32-34. At this temperature the spawn can be kept for 2 to 4 months.
Edited by Groo (02/22/15 04:09 PM)
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 8 years, 10 months
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Re: Alternatives for long term myc storage? [Re: Groo]
#21313706 - 02/22/15 04:09 PM (9 years, 10 months ago) |
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like gerber? lol
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,313
Last seen: 5 months, 30 days
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Re: Alternatives for long term myc storage? [Re: Mushroom_J]
#21313723 - 02/22/15 04:15 PM (9 years, 10 months ago) |
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Edited by Groo (02/22/15 04:23 PM)
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 8 years, 10 months
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Re: Alternatives for long term myc storage? [Re: Groo]
#21313756 - 02/22/15 04:27 PM (9 years, 10 months ago) |
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I don't have money. That's why I'm looking for an alternative. Stuff that I already have that others may have used. I personally don't like ordering anything online.
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: Alternatives for long term myc storage? [Re: Mushroom_J]
#21313911 - 02/22/15 05:13 PM (9 years, 10 months ago) |
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Test tubs would be more appropriate
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Alternatives for long term myc storage? [Re: Mushroom_J]
#21313924 - 02/22/15 05:15 PM (9 years, 10 months ago) |
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You can keep plates in the fridge for awhile.
I've fruited cultures from clone plates that spent 6+ months in there.
It helps to store them inside a little insulated lunch box.
Really the best option is to make proper slants though.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,350
Loc: Dept of know what I'm say...
Last seen: 37 minutes, 1 second
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Re: Alternatives for long term myc storage? [Re: PirateSwazey]
#21314137 - 02/22/15 05:55 PM (9 years, 10 months ago) |
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I've stored grain masters for 8 months. Could have probably went longer
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Alternatives for long term myc storage? [Re: Stromrider]
#21314172 - 02/22/15 06:01 PM (9 years, 10 months ago) |
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I believe that grain masters store very well. Biggest issue is refrigerator space.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Alternatives for long term myc storage? [Re: Pastywhyte]
#21314247 - 02/22/15 06:18 PM (9 years, 10 months ago) |
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Slant tubes are a little pricey. I just blew a good chunk of money on slants myself haha
How long are you going to be without money? Im sure you could save up 20 bux for a ten pack of proper tubes in a few months time Just store plates for now, pull it out and make a transfer if you need
--------------------

Amateur Mycologists United
AMU Q&A
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: Alternatives for long term myc storage? [Re: mushpunx]
#21314465 - 02/22/15 06:56 PM (9 years, 10 months ago) |
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Are slant tubes kinda like a test tube.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Alternatives for long term myc storage? [Re: TrIpPyDuDe]
#21314468 - 02/22/15 06:57 PM (9 years, 10 months ago) |
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Yes.
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: Alternatives for long term myc storage? [Re: Pastywhyte]
#21314494 - 02/22/15 07:02 PM (9 years, 10 months ago) |
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Gotcha now
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 8 years, 10 months
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Re: Alternatives for long term myc storage? [Re: TrIpPyDuDe]
#21314761 - 02/22/15 08:04 PM (9 years, 10 months ago) |
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How long without money? lol.. Until I find a job. Hasn't been going well. tax return maybe. I guess i'll keep plates after i test each one... then buy real shit.
I suppose a grain master could work.. then transfer single grains to plates or something like that... then to slants when I have cash.
I'd still like to figure out a grocery store "tek" for long term storage. You can get everything there cept for slants... agar, tongue depressors etc. We'll see what happens.
Thanks guys.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Alternatives for long term myc storage? [Re: Mushroom_J]
#21314836 - 02/22/15 08:19 PM (9 years, 10 months ago) |
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There are small vials for essential oils, like coke vials. You can get them cheap sometimes, but they are short. Might be able to find a taller one
How many cultures do you need to slant? Any cheap test tube with a cap should work. Maybe vacutainers for drawing blood... they have a rubber stopper in the top that fits air tight. BD makes a good one
--------------------

Amateur Mycologists United
AMU Q&A
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 8 years, 10 months
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Re: Alternatives for long term myc storage? [Re: mushpunx]
#21314986 - 02/22/15 08:51 PM (9 years, 10 months ago) |
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Well.. lol... as many as i can get. I like agar work.. surprisingly. for now storing plates will work till i have some dough... or a grain master.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,350
Loc: Dept of know what I'm say...
Last seen: 37 minutes, 1 second
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Re: Alternatives for long term myc storage? [Re: Pastywhyte]
#21316002 - 02/23/15 04:12 AM (9 years, 10 months ago) |
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Quote:
Pastywhyte said: I believe that grain masters store very well. Biggest issue is refrigerator space.
I have a dedicated culture fridge in my basement. I have a problem
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: Alternatives for long term myc storage? [Re: Stromrider]
#21316013 - 02/23/15 04:20 AM (9 years, 10 months ago) |
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Quote:
Stromrider said:
Quote:
Pastywhyte said: I believe that grain masters store very well. Biggest issue is refrigerator space.
I have a dedicated culture fridge in my basement. I have a problem 
I've got a mini one i'm starting to feel up.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,350
Loc: Dept of know what I'm say...
Last seen: 37 minutes, 1 second
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Re: Alternatives for long term myc storage? [Re: TrIpPyDuDe]
#21316016 - 02/23/15 04:21 AM (9 years, 10 months ago) |
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Gong to have to go full size!
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: Alternatives for long term myc storage? [Re: Stromrider]
#21316019 - 02/23/15 04:25 AM (9 years, 10 months ago) |
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Quote:
Stromrider said: Gong to have to go full size! 
I know it! I want to get all the LC's i can. And i want to do the same with spores.
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psyder
The Apprentice


Registered: 09/16/14
Posts: 220
Last seen: 2 years, 8 months
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Re: Alternatives for long term myc storage? [Re: TrIpPyDuDe]
#21316030 - 02/23/15 04:41 AM (9 years, 10 months ago) |
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Wrap your (preferably) full grown petry in peroxidated clingwrap. Then open it a bit stretching the clingwrap just so a needle can pass. Do it and inject 5 ml sterile distilled water (tap should work too) and while taking water back try to take all of the hypae off agar into srynge. Put that water in something sterile and airtight. By source literature it should go in stasis lacking nutes and air and will keep viable atleast 5 years on 4C temp. I guess you have your sterility on spot so you can skip the clingwrap, it was something I would do when I was carefull making LI. Good luck.
Also, just droping agar pieces with myc in water will also work, tht was what the authors did.
For most basidia viability has been repoted as long as 10 years. Oysters having around 78% recovery after 10 years period.
Hint: Use regular unfiltered jars and seal with wax
Edited by psyder (02/23/15 04:57 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Alternatives for long term myc storage? [Re: Stromrider]
#21316038 - 02/23/15 04:50 AM (9 years, 10 months ago) |
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Quote:
Stromrider said:
Quote:
Pastywhyte said: I believe that grain masters store very well. Biggest issue is refrigerator space.
I have a dedicated culture fridge in my basement. I have a problem 
Me too, its full
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psyder
The Apprentice


Registered: 09/16/14
Posts: 220
Last seen: 2 years, 8 months
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Re: Alternatives for long term myc storage? [Re: Pastywhyte]
#21316047 - 02/23/15 04:58 AM (9 years, 10 months ago) |
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Quote:
Pastywhyte said:
Quote:
Stromrider said:
Quote:
Pastywhyte said: I believe that grain masters store very well. Biggest issue is refrigerator space.
I have a dedicated culture fridge in my basement. I have a problem 
Me too, its full 
I envy your problems Hehehe
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