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InvisibleMushenstein
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Registered: 02/19/15
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Loc: At The Gates
Strain performance for LC
    #21312114 - 02/22/15 09:24 AM (9 years, 10 months ago)

Sorry if this was asked before. I couldn't find it anywhere.

Do different strains perform differently in LC's?

Second, What would cause me to get s LC success rate of 1 out of 3. I can see well water appears to work better then tap or bottled, but if the parameters are the same why would I get a 30% success rate if using the same strain?

PESA seems to love LC while PE freggin hates it. Is this right?

Success rate with PE seems to be 1 out of 5. What gives. I used the same ingred's for all, and none show contam. Some take and some don't.

Looking Good


Starting good


PESA doing fine


PE Just wont take off




One PE started but very slow


Nothing PESA


PE envious of life


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InvisiblebodhisattaMDiscordReddit
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Re: Strain performance for LC [Re: Mushenstein]
    #21312124 - 02/22/15 09:29 AM (9 years, 10 months ago)

that's what seems to be most peoples success rate with LC hence why most of the time they're strongly suggested against being used. especially with shitty lids like those too, you want a syringe filter or something that won't ever get wet if the LC sloshes around or boils in the PC.

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InvisibleMushenstein
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Re: Strain performance for LC [Re: bodhisatta]
    #21312140 - 02/22/15 09:36 AM (9 years, 10 months ago)

I had no Idea that was a normal success rate. Seems like a lot of spores being wasted. I guess agar would be better or G2G. I'm not ready for agar though.I just heard people going on about LC.

Also, the lids are what I saw on the Teks and YouTube. I guess I should invest some loot in the real thing and quit nickel and diming it.

Thanks for you reply.


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InvisiblebodhisattaMDiscordReddit
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Re: Strain performance for LC [Re: Mushenstein]
    #21312146 - 02/22/15 09:39 AM (9 years, 10 months ago)

I have never seen a good TEK on youtube other than let's grow mushrooms that got pirated and put up there. every other one is just some dumb kids video. some of them even look semi-professional but then are just riddled with bad habbits and technique.

starting a LC from spores is just asking for trouble. you start a LC with agar. and if you're using agar already then LC is sort of pointless. LC is just an extra step if you're starting with spores. you would be quicker if you just shoot up your cakes with the spores.

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InvisibleMushenstein
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Re: Strain performance for LC [Re: bodhisatta]
    #21312159 - 02/22/15 09:44 AM (9 years, 10 months ago)

I was going for making my syringes lasting longer. I went through 4 syringes in a week and figured I should try to stretch it out.

I just don't know if I can pull agar off. I bought some red algae agar agar in the health food store but I haven't got the courage to start mixing it up.

Does agar stretch out syringes. You know, make um last longer?


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InvisibleMushenstein
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Re: Strain performance for LC [Re: bodhisatta]
    #21312169 - 02/22/15 09:47 AM (9 years, 10 months ago)

Yeah, I was watching noobs on Youtube, like that bearded wonder idiot with the LC's and birdseed. Just trying to learn.


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Offlinespacechildo
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Registered: 01/24/13
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Re: Strain performance for LC [Re: Mushenstein]
    #21312174 - 02/22/15 09:51 AM (9 years, 10 months ago)

if you want your syringes to last just knock up some brf cakes and print the fruits,
you'll have infinite amount of spores to put in your syringes :shrug:

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InvisibleMushenstein
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Re: Strain performance for LC [Re: bodhisatta]
    #21312184 - 02/22/15 09:53 AM (9 years, 10 months ago)

you said agar to LC. Would I just cut a slab of myc off the agar and throw it in the LC? Does the agar gelatin dissolve in the LC solution?
or should I cut right through the myc in the agar to expose it to the solution. Is this how you do agar on grain as well?


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InvisibleMushenstein
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Re: Strain performance for LC [Re: spacechildo]
    #21312190 - 02/22/15 09:55 AM (9 years, 10 months ago)

I knocked p 24 cakes and their still colonizing. I haven't got to fruit yet. But I'm excited as shit to start.


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InvisibleGrey
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Re: Strain performance for LC [Re: Mushenstein]
    #21312277 - 02/22/15 10:21 AM (9 years, 10 months ago)

Agar will stretch your syringes a lot. You only need one drop per. I haven't even touched my syringes because I've just been swabbing/scraping prints to agar. The tiniest bit germinated. Most of my prints look untouched even though I've got six plates started from it.


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InvisiblebodhisattaMDiscordReddit
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Re: Strain performance for LC [Re: Grey]
    #21312417 - 02/22/15 11:01 AM (9 years, 10 months ago)

the link to my unfinished spore harvesting is in my signature you can read through that.

you only need a few drops of a spore syringe to start a grow. a single syringe can do 100 cakes easily if you use a few drops per hole. but I would start taking prints and or spore swabs. experiment. you're only going to get good with practice, everyone fucks up a few times their first few times. agar also is good to visualize contamination before using inoculant. without agar you're flying blind. you'll get the hang of it :thumbup:

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Offlineinvitro

Registered: 05/03/13
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Re: Strain performance for LC [Re: Mushenstein]
    #21312719 - 02/22/15 12:03 PM (9 years, 10 months ago)

I'm curious about solving this problem...
What is your nutrient solution, what concentration?

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InvisibleVal
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
Re: Strain performance for LC [Re: Mushenstein]
    #21313617 - 02/22/15 03:34 PM (9 years, 10 months ago)

Quote:

Jimmy Slump said:
I just don't know if I can pull agar off. I bought some red algae agar agar in the health food store but I haven't got the courage to start mixing it up.





Well whether you can or cant pull it off is up to you, Its not rocket science...its more about technique and cleanliness...and you will know how you score. Once you master (learn) agar technique it opens the door to many more possibilities.

All the info you need to know is at your fingertips (on this great site) about learning agar...theres even videos by Rodger Rabbit that will help.

Here is a link I saw the other day that might nudge you in the right direction http://www.shroomery.org/forums/showflat.php/Number/21176491#21176491

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Offlinespacechildo
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Re: Strain performance for LC [Re: Val]
    #21313633 - 02/22/15 03:40 PM (9 years, 10 months ago)

if you can make jello you know how to make agar.
if you can use a knife to cut through things you know how to use agar :shrug:

now just apply said knowledge inside a SAB and get to it! nothing to be afraid of!

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InvisibleNeomorph
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Registered: 12/10/14
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Loc: Europe
Re: Strain performance for LC [Re: Mushenstein]
    #21313731 - 02/22/15 04:17 PM (9 years, 10 months ago)

Why dont u try Grain LC AGAR'S GRAIN LC

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InvisiblebodhisattaMDiscordReddit
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Re: Strain performance for LC [Re: Neomorph]
    #21313975 - 02/22/15 05:26 PM (9 years, 10 months ago)

grain LC is a pretty fucking stupid idea if you don't feel comfortable with agar. a regular old LC is a better idea than a grain LC

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InvisibleNeomorph
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Re: Strain performance for LC [Re: bodhisatta]
    #21314028 - 02/22/15 05:34 PM (9 years, 10 months ago)

why is that ? it seem way more easy thats why i suggested :smile:

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Offlinespacechildo
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Re: Strain performance for LC [Re: bodhisatta]
    #21314038 - 02/22/15 05:35 PM (9 years, 10 months ago)

easiest doesnt mean safest, grains have nasty sturdy endospores and all this is still in the equation:

Quote:

bodhisatta said:
shitty lids like those too, you want a syringe filter or something that won't ever get wet if the LC sloshes around or boils in the PC.




Quote:

bodhisatta said:
starting a LC from spores is just asking for trouble. you start a LC with agar. and if you're using agar already then LC is sort of pointless.



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Offlineinvitro

Registered: 05/03/13
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Re: Strain performance for LC [Re: bodhisatta]
    #21314040 - 02/22/15 05:36 PM (9 years, 10 months ago)

I think grain lc is a really bad idea, and I like lcs as a concept.  Grain jars often harbor atleast some bacteria/mold that have been knocked down by the pressure cooking, but they can bounce back after time and remain invisible/insignificant in the jar, but when turned into a lc they can take off and ruin it.  Proceed with caution.

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InvisibleNeomorph
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Re: Strain performance for LC [Re: invitro]
    #21314072 - 02/22/15 05:43 PM (9 years, 10 months ago)

the contams are the only reason, or even spores become weacker or something ?

Edited by Neomorph (02/22/15 05:44 PM)

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