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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Strain performance for LC
#21312114 - 02/22/15 09:24 AM (9 years, 10 months ago) |
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Sorry if this was asked before. I couldn't find it anywhere.
Do different strains perform differently in LC's?
Second, What would cause me to get s LC success rate of 1 out of 3. I can see well water appears to work better then tap or bottled, but if the parameters are the same why would I get a 30% success rate if using the same strain?
PESA seems to love LC while PE freggin hates it. Is this right?
Success rate with PE seems to be 1 out of 5. What gives. I used the same ingred's for all, and none show contam. Some take and some don't.
Looking Good

Starting good

PESA doing fine

PE Just wont take off


One PE started but very slow 
Nothing PESA

PE envious of life
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Strain performance for LC [Re: Mushenstein]
#21312124 - 02/22/15 09:29 AM (9 years, 10 months ago) |
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that's what seems to be most peoples success rate with LC hence why most of the time they're strongly suggested against being used. especially with shitty lids like those too, you want a syringe filter or something that won't ever get wet if the LC sloshes around or boils in the PC.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: bodhisatta]
#21312140 - 02/22/15 09:36 AM (9 years, 10 months ago) |
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I had no Idea that was a normal success rate. Seems like a lot of spores being wasted. I guess agar would be better or G2G. I'm not ready for agar though.I just heard people going on about LC.
Also, the lids are what I saw on the Teks and YouTube. I guess I should invest some loot in the real thing and quit nickel and diming it.
Thanks for you reply.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
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Re: Strain performance for LC [Re: Mushenstein]
#21312146 - 02/22/15 09:39 AM (9 years, 10 months ago) |
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I have never seen a good TEK on youtube other than let's grow mushrooms that got pirated and put up there. every other one is just some dumb kids video. some of them even look semi-professional but then are just riddled with bad habbits and technique.
starting a LC from spores is just asking for trouble. you start a LC with agar. and if you're using agar already then LC is sort of pointless. LC is just an extra step if you're starting with spores. you would be quicker if you just shoot up your cakes with the spores.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: bodhisatta]
#21312159 - 02/22/15 09:44 AM (9 years, 10 months ago) |
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I was going for making my syringes lasting longer. I went through 4 syringes in a week and figured I should try to stretch it out.
I just don't know if I can pull agar off. I bought some red algae agar agar in the health food store but I haven't got the courage to start mixing it up.
Does agar stretch out syringes. You know, make um last longer?
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: bodhisatta]
#21312169 - 02/22/15 09:47 AM (9 years, 10 months ago) |
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Yeah, I was watching noobs on Youtube, like that bearded wonder idiot with the LC's and birdseed. Just trying to learn.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Strain performance for LC [Re: Mushenstein]
#21312174 - 02/22/15 09:51 AM (9 years, 10 months ago) |
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if you want your syringes to last just knock up some brf cakes and print the fruits, you'll have infinite amount of spores to put in your syringes
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: bodhisatta]
#21312184 - 02/22/15 09:53 AM (9 years, 10 months ago) |
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you said agar to LC. Would I just cut a slab of myc off the agar and throw it in the LC? Does the agar gelatin dissolve in the LC solution? or should I cut right through the myc in the agar to expose it to the solution. Is this how you do agar on grain as well?
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: spacechildo]
#21312190 - 02/22/15 09:55 AM (9 years, 10 months ago) |
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I knocked p 24 cakes and their still colonizing. I haven't got to fruit yet. But I'm excited as shit to start.
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Grey
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Registered: 11/06/14
Posts: 6,223
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Re: Strain performance for LC [Re: Mushenstein]
#21312277 - 02/22/15 10:21 AM (9 years, 10 months ago) |
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Agar will stretch your syringes a lot. You only need one drop per. I haven't even touched my syringes because I've just been swabbing/scraping prints to agar. The tiniest bit germinated. Most of my prints look untouched even though I've got six plates started from it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Strain performance for LC [Re: Grey]
#21312417 - 02/22/15 11:01 AM (9 years, 10 months ago) |
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the link to my unfinished spore harvesting is in my signature you can read through that.
you only need a few drops of a spore syringe to start a grow. a single syringe can do 100 cakes easily if you use a few drops per hole. but I would start taking prints and or spore swabs. experiment. you're only going to get good with practice, everyone fucks up a few times their first few times. agar also is good to visualize contamination before using inoculant. without agar you're flying blind. you'll get the hang of it
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Strain performance for LC [Re: Mushenstein]
#21312719 - 02/22/15 12:03 PM (9 years, 10 months ago) |
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I'm curious about solving this problem... What is your nutrient solution, what concentration?
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Val
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
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Re: Strain performance for LC [Re: Mushenstein]
#21313617 - 02/22/15 03:34 PM (9 years, 10 months ago) |
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Quote:
Jimmy Slump said: I just don't know if I can pull agar off. I bought some red algae agar agar in the health food store but I haven't got the courage to start mixing it up.
Well whether you can or cant pull it off is up to you, Its not rocket science...its more about technique and cleanliness...and you will know how you score. Once you master (learn) agar technique it opens the door to many more possibilities.
All the info you need to know is at your fingertips (on this great site) about learning agar...theres even videos by Rodger Rabbit that will help.
Here is a link I saw the other day that might nudge you in the right direction http://www.shroomery.org/forums/showflat.php/Number/21176491#21176491
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Strain performance for LC [Re: Val]
#21313633 - 02/22/15 03:40 PM (9 years, 10 months ago) |
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if you can make jello you know how to make agar. if you can use a knife to cut through things you know how to use agar 
now just apply said knowledge inside a SAB and get to it! nothing to be afraid of!
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: Mushenstein]
#21313731 - 02/22/15 04:17 PM (9 years, 10 months ago) |
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Why dont u try Grain LC AGAR'S GRAIN LC
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Strain performance for LC [Re: Neomorph]
#21313975 - 02/22/15 05:26 PM (9 years, 10 months ago) |
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grain LC is a pretty fucking stupid idea if you don't feel comfortable with agar. a regular old LC is a better idea than a grain LC
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: bodhisatta]
#21314028 - 02/22/15 05:34 PM (9 years, 10 months ago) |
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why is that ? it seem way more easy thats why i suggested
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Last seen: 7 years, 4 months
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Re: Strain performance for LC [Re: bodhisatta]
#21314038 - 02/22/15 05:35 PM (9 years, 10 months ago) |
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easiest doesnt mean safest, grains have nasty sturdy endospores and all this is still in the equation:
Quote:
bodhisatta said: shitty lids like those too, you want a syringe filter or something that won't ever get wet if the LC sloshes around or boils in the PC.
Quote:
bodhisatta said: starting a LC from spores is just asking for trouble. you start a LC with agar. and if you're using agar already then LC is sort of pointless.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Strain performance for LC [Re: bodhisatta]
#21314040 - 02/22/15 05:36 PM (9 years, 10 months ago) |
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I think grain lc is a really bad idea, and I like lcs as a concept. Grain jars often harbor atleast some bacteria/mold that have been knocked down by the pressure cooking, but they can bounce back after time and remain invisible/insignificant in the jar, but when turned into a lc they can take off and ruin it. Proceed with caution.
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: invitro]
#21314072 - 02/22/15 05:43 PM (9 years, 10 months ago) |
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the contams are the only reason, or even spores become weacker or something ?
Edited by Neomorph (02/22/15 05:44 PM)
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Strain performance for LC [Re: Neomorph]
#21314125 - 02/22/15 05:53 PM (9 years, 10 months ago) |
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Contams are the only reason. if it weren't for that everyone would use GLC.
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: invitro]
#21314336 - 02/22/15 06:34 PM (9 years, 10 months ago) |
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blackdust said:
Quote:
By using this method and taking advantage of the SHIP in the jars I was able to bring my success rate of about 85% of doing open air G2G (bacteria contams suck) to 100% using this LC in open air
he said that for GLC!!
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: Strain performance for LC [Re: Neomorph]
#21314425 - 02/22/15 06:51 PM (9 years, 10 months ago) |
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Blackdust wants contams, we want mushrooms 
its still LC from spores here and still no proper filters, I'm not sure what you think you are debating?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Strain performance for LC [Re: spacechildo]
#21314493 - 02/22/15 07:02 PM (9 years, 10 months ago) |
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I can find you a lot of people saying a lot of things. but I can't find you a paper trail of all those things people say working well all the time.
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: bodhisatta]
#21314559 - 02/22/15 07:17 PM (9 years, 10 months ago) |
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im new here so im just trying to learn the best methods so thats all, thanks for the infos
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Strain performance for LC [Re: Neomorph]
#21314639 - 02/22/15 07:40 PM (9 years, 10 months ago) |
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Hey OP you grow those poppies yourself? Tea drinker?
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Strain performance for LC [Re: mushpunx]
#21314682 - 02/22/15 07:49 PM (9 years, 10 months ago) |
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what poppies, i still didnt start growing shroomies, i still have some problems with finding spores :/
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Strain performance for LC [Re: Neomorph]
#21314754 - 02/22/15 08:03 PM (9 years, 10 months ago) |
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Quote:
kastriotr said: what poppies, i still didnt start growing shroomies, i still have some problems with finding spores :/
OP= Original poster. His sig is opium poppies.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: Val]
#21316292 - 02/23/15 07:33 AM (9 years, 10 months ago) |
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Thanks for all you reply's. I was going to try the Pasty white method of agar tonight. What do you guys think of that method? Any pit falls that need be avoided.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: mushpunx]
#21316317 - 02/23/15 07:46 AM (9 years, 10 months ago) |
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There are a couple great books on growing poppies. If anyone is interested I will post the names tonight. I don't have them on me right now.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: Strain performance for LC [Re: Mushenstein]
#21316323 - 02/23/15 07:47 AM (9 years, 10 months ago) |
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pasty plates are great, remember the TP for less condensation!
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: spacechildo]
#21316346 - 02/23/15 07:55 AM (9 years, 10 months ago) |
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TP? toilet paper, or paper towels? I think he put it on top in the PC. Is this what you mean?
Im starting the agar in these: they were like 2 bucks for 8 of them.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Strain performance for LC [Re: Mushenstein]
#21316353 - 02/23/15 07:58 AM (9 years, 10 months ago) |
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tp/pt doesnt matter. on top of the container lid, under the foil just as described in his tek!
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Strain performance for LC [Re: spacechildo]
#21316383 - 02/23/15 08:08 AM (9 years, 10 months ago) |
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Awesome possum. I'll get it done tonight and post pics of my successes and failures!
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Strain performance for LC [Re: Mushenstein]
#21317077 - 02/23/15 11:00 AM (9 years, 10 months ago) |
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I'm interested In that literature.
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AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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