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Offlinefallouton420
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Registered: 01/28/14
Posts: 56
Last seen: 1 year, 4 months
started sterilizing but had to stop
    #21311700 - 02/22/15 04:01 AM (9 years, 2 months ago)

I started steaming my BRF cakes, but due to timing I had to stop sterilizing about 5-10 minutes after I started, should I dump these cakes or are they ok?


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All the things I have said am saying or will say are to be discredited and taken as false, due to an overactive imagination trying to imagine what it would be like in these actual situations

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Offlinefallouton420
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Registered: 01/28/14
Posts: 56
Last seen: 1 year, 4 months
Re: started sterilizing but had to stop [Re: fallouton420]
    #21311712 - 02/22/15 04:10 AM (9 years, 2 months ago)

I should've been more clear, is it ok if I sterilize them within a couple days before I use them or should I just not use them at all?


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All the things I have said am saying or will say are to be discredited and taken as false, due to an overactive imagination trying to imagine what it would be like in these actual situations

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Offliner00tuuu123
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Re: started sterilizing but had to stop [Re: fallouton420]
    #21311809 - 02/22/15 05:56 AM (9 years, 2 months ago)

Quote:

fallouton420 said:
I should've been more clear, is it ok if I sterilize them within a couple days before I use them or should I just not use them at all?



:huxleyfacepalm: just finnish and toss em in the fridge till 1 day before you need them. This ain't rocket surgery! :gc:


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:kingcrankey: Please report me to a Mod for hurting your punk ass hippie feelings :flipthebird: And all time Champion thread killer.:thatsayes:

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Invisiblehamloaf
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Re: started sterilizing but had to stop [Re: r00tuuu123]
    #21311823 - 02/22/15 06:14 AM (9 years, 2 months ago)

"rocket surgery".  :lol:  I didn't know rockets require surgery.  I know rockets require scientists though.

OP, You will be fine as long as you plan on sterilizing those mediums within the next few days.  Sometime I am constrained by the happenings of life and have to shutdown the sterilization-cycle right in the middle of cycle to deal with an issue or something. 

When that happens I just reschedule a sterilization run the next morning.  If you are super worried, you can give the mediums 30 extra minutes in the sterilizer as a precaution to safety measures.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinesporeguy
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Registered: 02/13/15
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Last seen: 9 years, 1 month
Re: started sterilizing but had to stop [Re: fallouton420]
    #21311825 - 02/22/15 06:18 AM (9 years, 2 months ago)

Quote:

fallouton420 said:
I started steaming my BRF cakes, but due to timing I had to stop sterilizing about 5-10 minutes after I started, should I dump these cakes or are they ok?




work on your timing - you need about 4hrs to fully set up, sterelise, and then clean up.

finish sterilising ASAP. if you wait more than a day or two all sorts of contams will blossom in the jars and may or may not be eradicated by the full sterilizsation.

as mentioned, once sterilized the jars can be stored until use for days or weeks.

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Offlinetourrat
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Registered: 01/16/14
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Re: started sterilizing but had to stop [Re: sporeguy]
    #21311854 - 02/22/15 06:43 AM (9 years, 2 months ago)

Don't grow mushies in your parents house yo.....just kidding.



But really don't grow mushies in your parents house lol.


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Knee deep in the hotel tub.....

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Invisiblehamloaf
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Re: started sterilizing but had to stop [Re: sporeguy]
    #21311882 - 02/22/15 07:09 AM (9 years, 2 months ago)

Quote:

sporeguy said:
as mentioned, once sterilized the jars can be stored until use for days or weeks.



No.  That is not good practice either. 

Waiting to inoculate freshly sterilized media gives the micro-beasties that were just knocked back by the sterilization cycle a chance to recover ruining the head start for your mushroom culture that was generated by sterilizing the media in the first place. 

Also, the moisture content of your sterile substrate becomes threatened the longer you wait to inoculate said mediums after sterilization.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinefallouton420
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Registered: 01/28/14
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Re: started sterilizing but had to stop [Re: hamloaf]
    #21312955 - 02/22/15 12:58 PM (9 years, 2 months ago)

Thanks for all the answers guys, this is my first time so I just wanted to make sure that the laws of the universe weren't being broken or anything :grin:


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All the things I have said am saying or will say are to be discredited and taken as false, due to an overactive imagination trying to imagine what it would be like in these actual situations

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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: started sterilizing but had to stop [Re: hamloaf]
    #21312988 - 02/22/15 01:10 PM (9 years, 2 months ago)

Quote:

THE_LOAF said:
Quote:

sporeguy said:
as mentioned, once sterilized the jars can be stored until use for days or weeks.



No.  That is not good practice either. 

Waiting to inoculate freshly sterilized media gives the micro-beasties that were just knocked back by the sterilization cycle a chance to recover ruining the head start for your mushroom culture that was generated by sterilizing the media in the first place. 

Also, the moisture content of your sterile substrate becomes threatened the longer you wait to inoculate said mediums after sterilization.




"Sterilization or sterilisation is a term referring to any process that eliminates (removes) or kills all forms of life, including transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.[1][2] Sterilization can be achieved with one or more of heat,chemicals, irradiation, high pressure, andfiltration."

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OfflinecronicrFacebook
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Re: started sterilizing but had to stop [Re: insanemike]
    #21313010 - 02/22/15 01:15 PM (9 years, 2 months ago)

Quote:

insanemike said:
Quote:

THE_LOAF said:
Quote:

sporeguy said:
as mentioned, once sterilized the jars can be stored until use for days or weeks.



No.  That is not good practice either. 

Waiting to inoculate freshly sterilized media gives the micro-beasties that were just knocked back by the sterilization cycle a chance to recover ruining the head start for your mushroom culture that was generated by sterilizing the media in the first place. 

Also, the moisture content of your sterile substrate becomes threatened the longer you wait to inoculate said mediums after sterilization.




"Sterilization or sterilisation is a term referring to any process that eliminates (removes) or kills all forms of life, including transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.[1][2] Sterilization can be achieved with one or more of heat,chemicals, irradiation, high pressure, andfiltration."



different meaning when it comes to our hobby"sterilization" is not taken to seriously all we get is a window long enough to colonize the substrate


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: started sterilizing but had to stop [Re: cronicr]
    #21313077 - 02/22/15 01:32 PM (9 years, 2 months ago)

Quote:

cronicr said:
Quote:

insanemike said:
Quote:

THE_LOAF said:
Quote:

sporeguy said:
as mentioned, once sterilized the jars can be stored until use for days or weeks.



No.  That is not good practice either. 

Waiting to inoculate freshly sterilized media gives the micro-beasties that were just knocked back by the sterilization cycle a chance to recover ruining the head start for your mushroom culture that was generated by sterilizing the media in the first place. 

Also, the moisture content of your sterile substrate becomes threatened the longer you wait to inoculate said mediums after sterilization.




"Sterilization or sterilisation is a term referring to any process that eliminates (removes) or kills all forms of life, including transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.[1][2] Sterilization can be achieved with one or more of heat,chemicals, irradiation, high pressure, andfiltration."



different meaning when it comes to our hobby"sterilization" is not taken to seriously all we get is a window long enough to colonize the substrate




On multiple occasions when I first began this hobby, I would leave a sterilized grain jar from a batch as that, sterile and have never seen or smelled a contam. I have done this in the past to ensure I was cooking my grain properly. So if I ended up with a contam, I knew it was from inoculation. But seeing how OP is merely steaming with no pressure, I guess it wouldn't be sterile.

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OfflinecronicrFacebook
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Re: started sterilizing but had to stop [Re: insanemike]
    #21313087 - 02/22/15 01:35 PM (9 years, 2 months ago)

even grains are not truly sterilized:super: af ew days is probably no biggie but weeks i would never do


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: started sterilizing but had to stop [Re: cronicr]
    #21313258 - 02/22/15 01:50 PM (9 years, 2 months ago)

Quote:

cronicr said:
even grains are not truly sterilized:super: af ew days is probably no biggie but weeks i never do




From my reading of this hobby and learning terms like pasteurization and sterilization, it is of my understanding that when referring to a window of opportunity one is referencing the use of  pasteurization. Perhaps we just use the wrong term?

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OfflinecronicrFacebook
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Re: started sterilizing but had to stop [Re: insanemike]
    #21313267 - 02/22/15 01:52 PM (9 years, 2 months ago)

naw what we would consider "sterilized" isn't sterilized by dictionary definitions but good enough for our use, to truly sterilize grains it would probably take 12 hours or something like that in the pc which by then the grain would be mush.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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