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Offlineboodog420
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pre-poured petri dishes
    #21300047 - 02/19/15 05:13 PM (9 years, 10 months ago)

So just got 4 pre-poured petri dishes pdya and a syringe of chicon nindo and tamp in the mail(from a sponsor). I'm just got a pc last weekend and did my first wbs jars on my own. Tryin  to step my game up so I don't have to buy so much when I can make it myself. My question is do I just open the dish and put a drop of spore solution in the center ??(and in a sab of course)

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OfflinecronicrMFacebook
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Re: pre-poured petri dishes [Re: boodog420]
    #21300082 - 02/19/15 05:20 PM (9 years, 10 months ago)

yep:super:i like to heat the needle and make a divot to keep the drop in place


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Offlineboodog420
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Re: pre-poured petri dishes [Re: cronicr]
    #21300176 - 02/19/15 05:42 PM (9 years, 10 months ago)

Gotcha thanks. Then hopefully it grows in a perfect myc circle(with no contams)then I cut pieces off to nock up jars with??

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OfflinePsilicon
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Re: pre-poured petri dishes [Re: boodog420]
    #21300198 - 02/19/15 05:49 PM (9 years, 10 months ago)

I'd do one transfer to make sure it's clean, but yeah.  :thumbup:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
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InvisibleFungus Mountain
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Re: pre-poured petri dishes [Re: cronicr]
    #21300288 - 02/19/15 06:14 PM (9 years, 10 months ago)

Quote:

cronicr said:
yep:super:i like to heat the needle and make a divot to keep the drop in place




Curious about this. So do you stick the center of your plate with your red hot needle to create the divot?


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“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
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InvisibleNativenglish
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Re: pre-poured petri dishes [Re: Psilicon]
    #21300335 - 02/19/15 06:24 PM (9 years, 10 months ago)

Quote:

van der griegen said:
I'd do one transfer to make sure it's clean, but yeah.  :thumbup:




Theoretically he could get three transfers if he is clean, using his four dishes. Is that correct?

I'm also interested in why he would not do 3 transfers if he could? Would it just not make enough difference in yield to the added work and contam possibility? ...he would need some more plates to make more transfers for a noticeable outcome?

When I re-read this I think it sounds smart-assed, but I really don't mean it like that at all. I am giving serious thought to ordering some pre-poured plates myself, and want to learn as much as possible. They are really not cheap and I would like to know a good calculus of how many plates to order for my purposes.  :sunny:


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The government which governs least, governs best...

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OfflinePenelopeMushroom
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Re: pre-poured petri dishes [Re: Fungus Mountain]
    #21300348 - 02/19/15 06:27 PM (9 years, 10 months ago)

Quote:

Fungus Mountain said:
Quote:

cronicr said:
yep:super:i like to heat the needle and make a divot to keep the drop in place




Curious about this. So do you stick the center of your plate with your red hot needle to create the divot?




That will work.

It will also cool down the tip of your needle so it doesn't damage the solution.


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Invisibleblindingleaf
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Re: pre-poured petri dishes [Re: PenelopeMushroom]
    #21300386 - 02/19/15 06:33 PM (9 years, 10 months ago)

Quote:

Theoretically he could get three transfers if he is clean, using his four dishes. Is that correct?

I'm also interested in why he would not do 3 transfers if he could? Would it just not make enough difference in yield to the added work and contam possibility? ...he would need some more plates to make more transfers for a noticeable outcome?




yes he could get 3 transfers if he wanted to.  he would be limiting genetics further.
the outcome could be better or equal, assuming in both cases the spawn is clean.
any MS is roll of the dice.  even isolates from spore are a roll of the dice.  if plates are costing that much, maybe not worth it.
but if u make ur own plates, cost is negligible.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleNativenglish
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Re: pre-poured petri dishes [Re: blindingleaf]
    #21300491 - 02/19/15 06:49 PM (9 years, 10 months ago)

Yeah blindingleaf, I get you fo sho, but I've had a run of contams on a couple of my projects lately, and I'm not too trusting of my own abilities to work so cleanly. Problem is, I'm desperate to begin working with agar, and I would love to take that whole element of sterile agar plate pouring out of the equation. I don't think I would get much past that little process on my own talents.  :lol:


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The government which governs least, governs best...

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InvisiblePNWMusicMaker
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Re: pre-poured petri dishes [Re: Nativenglish]
    #21300505 - 02/19/15 06:50 PM (9 years, 10 months ago)

Just want to say agar can be really cheap and easy to play around with. I just wrote up my method which cost me less than 20 dollars to get going.
Check my sig link if you're of a mind to.


--------------------
Live to change, change to live.





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Offlineboodog420
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Re: pre-poured petri dishes [Re: Nativenglish]
    #21300518 - 02/19/15 06:53 PM (9 years, 10 months ago)

I don't really get the transfer thing . if its clean shouldn't I just use it?? I was planning on nockin up 2 of each strain, is that not a good idea??

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Invisibleblindingleaf
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Re: pre-poured petri dishes [Re: Nativenglish]
    #21300519 - 02/19/15 06:53 PM (9 years, 10 months ago)

have u looked at pasty plates?
pasty white easy agar TEK?

u don't pour there after pc, u put everything in a small container first, then sterilize it.  less messy, easier, just as good in many ways.  great tek, has gotten MANY MANY people into agar.  check it out if u can, I'm positive u could pull it off,  its worth it!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleNativenglish
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Re: pre-poured petri dishes [Re: blindingleaf]
    #21300610 - 02/19/15 07:16 PM (9 years, 10 months ago)

Quote:

blindingleaf said:
have u looked at pasty plates?
pasty white easy agar TEK?

u don't pour there after pc, u put everything in a small container first, then sterilize it.  less messy, easier, just as good in many ways.  great tek, has gotten MANY MANY people into agar.  check it out if u can, I'm positive u could pull it off,  its worth it!




Yeah! That is the ONLY way I can conceivably do agar. I have looked at that very carefully. Pasty put together a gem in that little TEK right there. Believe me, when I get the bollocks up to make my own.......it's going to be with Pasty's Easy Agar TEK for sure.


@Boodog420:  The reason to make transfers is to only transfer the MOST robust mycelium in the dish......to the next dish. Each transfer you make, the more of the less dominant myc will be left behind. In practice, you can do this maybe 15-20 times before you get a good, clean isolate.

It's a crapshoot, because you don't know what kind of fruit bodies the isolate will produce until you've actually cycled the whole thing and got it to a fruiting chamber and see your results. If it works well, it's SUPER.......but if it doesn't, it is a huge waste of time and material.


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The government which governs least, governs best...

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InvisiblePNWMusicMaker
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Re: pre-poured petri dishes [Re: Nativenglish]
    #21300668 - 02/19/15 07:30 PM (9 years, 10 months ago)

I don't use a PC in my tek, or a SAB for that matter. Mostly cause I'm a poor bastard and doing this all as cheaply as I can.


--------------------
Live to change, change to live.





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Offlineboodog420
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Re: pre-poured petri dishes [Re: PNWMusicMaker]
    #21300686 - 02/19/15 07:33 PM (9 years, 10 months ago)

I see now. Thanks for the info. Wouldn't it be better to do a couple transfers then fruit it and take material from a good fruit to put back on the plate ?? Or is that doable ??

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InvisiblePNWMusicMaker
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Re: pre-poured petri dishes [Re: boodog420]
    #21300711 - 02/19/15 07:40 PM (9 years, 10 months ago)

All a matter of preference I suppose. I had been, up until recently, working only with wild collected actives from WA. Now that I have some cubes going I plan on continuing to try new things.

I would hesitantly say yes, what you are talking about is totally doable.


--------------------
Live to change, change to live.





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Invisibleblindingleaf
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Re: pre-poured petri dishes [Re: boodog420]
    #21300734 - 02/19/15 07:45 PM (9 years, 10 months ago)

in theory yes, that makes sense to do, because we limit genetics further in hopes that those faster, rhizomorphic traits translate into great performance.
but sometimes it doesn't always work, and we could have been just as well off with 1-2 transfers instead of 3-4, assuming spawn is clean in both cases.
taking material from good fruit is always a good idea, be it from MS>straight to jar, or MS>several transfers on agar>jar.
in all cases, the clone should be tested for performance.
i personally do prefer to transfer a few time before grain.
other may transfer once to make sure its clean, then go to grain.
both ways have an equal chance in terms of performance and yield in theory.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Offlineboodog420
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Re: pre-poured petri dishes [Re: blindingleaf]
    #21300795 - 02/19/15 07:59 PM (9 years, 10 months ago)

Good info. Luckily I haven't had bad luck with contams yet (knock on wood) except for fruit fly larvae fuckin up my first go at brf jars. I've been using honey lc from just boiling water (noob tek I saw on here awhile back) , but amazed at how much faster it colonized my jars. I just wanna make sure I know its clean versus just hoping with my lc(from what I've read you cant really see contams in a lc)

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Invisibleblindingleaf
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Re: pre-poured petri dishes [Re: boodog420]
    #21300840 - 02/19/15 08:09 PM (9 years, 10 months ago)

i mean, by all means utilize a clean LC.
but also be careful with them.
i have had good ones go bad after one pull.
i have had good ones stay good after 8 pulls.

just be careful.  good sterile technique is ur best friend :thumbup:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Offlineboodog420
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Re: pre-poured petri dishes [Re: blindingleaf]
    #21300879 - 02/19/15 08:17 PM (9 years, 10 months ago)

True that ! I stay pretty small so not like I'd nock up 20 quarts at a time. But I'd rather start my lc with something I know is clean instead of gambling . crazy how quick it goes from buying everything and just shooting some spores to making everything yourself and only buying grain. I feel way more rewarded doing it all myself :-) wouldn't have thought I would be doin this 7 months ago when I ordered my first grow kit because I wanted to trip

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