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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Let me pick your brain..BRf cake transfer
#21299604 - 02/19/15 03:27 PM (9 years, 10 months ago) |
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I am thinking about cutting a few little chunks off of a colonized cake to either transfer on to agar or lc. At this point I'm just experimenting and learning new tricks. Any thoughts?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Let me pick your brain..BRf cake transfer [Re: blazedup]
#21299612 - 02/19/15 03:29 PM (9 years, 10 months ago) |
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You'd have to birth in an SAB or rip the cake apart.
I did this and got my PR culture:
Quote:
taGyo said: Experiments
12/4/14
So I recently finished my first cake. It didn't produce what I wanted because when I started this project I wasn't as knowledgable as I am now. Nevertheless and for the sake of science (and ) I decided to try cutting open my cake to look inside of it and see if I could visually see any contaminations. My first PC Puerto Rico cake had a bad bacteria infection and his brothers and sisters got mold before they were even birthed. So I got my stuff together:

Prepped my hands, picked up the cake and moved it to my SAB:

Flame sterilized my knife, cooled it on a spare agar plate and cut the cake in half carefully:
 
After that I took the half that didn't have a stroma bubble coming out of it and cut into it until I found this:
Took a sample from that. Skinned where I saw yellow contams and found cake underneath it, nothing to write home about. Started peeling off the verm to get a better look at the actual cake and I could see white, semi-healthy myc underneath it.
Cool, , now for the hard part.
This bubble is a contamination:

I saved this side for last so I could get used to using my knife. The contam grew on both sides but after I cut them all this one resurfaced. At one point I thought I had aerial myc but it turns out it was a reaction to the bacteria. This cake suffered :[. So I carefully took that blotch off and peeled away the verm. Nothing unusual. Started cutting very precise layers so I could get a good look inside. Nothing. FInally I took a piece of the now skinned down and broken up cake and cracked it in half:

Cracked it again, sterilized my knife and cut a piece out for a second tissue sample:

Final Pic:

I did a bunch of agar plates and ended last night at 4 am , Up at 9. More agar to make tonight 
UPDATE 1:
Both grew out. One got this ugly contam:

Transfered away on 12/11 so we'll see how these two grow.
I think this might be the final transfer boys:
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: Let me pick your brain..BRf cake transfer [Re: taGyo]
#21299679 - 02/19/15 03:41 PM (9 years, 10 months ago) |
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Quote:
taGyo said: You'd have to birth in an SAB or rip the cake apart.
I did this and got my PR culture:
Quote:
taGyo said: Experiments
12/4/14
So I recently finished my first cake. It didn't produce what I wanted because when I started this project I wasn't as knowledgable as I am now. Nevertheless and for the sake of science (and ) I decided to try cutting open my cake to look inside of it and see if I could visually see any contaminations. My first PC Puerto Rico cake had a bad bacteria infection and his brothers and sisters got mold before they were even birthed. So I got my stuff together:

Prepped my hands, picked up the cake and moved it to my SAB:

Flame sterilized my knife, cooled it on a spare agar plate and cut the cake in half carefully:
 
After that I took the half that didn't have a stroma bubble coming out of it and cut into it until I found this:
Took a sample from that. Skinned where I saw yellow contams and found cake underneath it, nothing to write home about. Started peeling off the verm to get a better look at the actual cake and I could see white, semi-healthy myc underneath it.
Cool, , now for the hard part.
This bubble is a contamination:

I saved this side for last so I could get used to using my knife. The contam grew on both sides but after I cut them all this one resurfaced. At one point I thought I had aerial myc but it turns out it was a reaction to the bacteria. This cake suffered :[. So I carefully took that blotch off and peeled away the verm. Nothing unusual. Started cutting very precise layers so I could get a good look inside. Nothing. FInally I took a piece of the now skinned down and broken up cake and cracked it in half:

Cracked it again, sterilized my knife and cut a piece out for a second tissue sample:

Final Pic:

I did a bunch of agar plates and ended last night at 4 am , Up at 9. More agar to make tonight 
UPDATE 1:
Both grew out. One got this ugly contam:

Transfered away on 12/11 so we'll see how these two grow.
I think this might be the final transfer boys:
Nice work...why not attempt to use a small piece instead of dissecting the whole cake? Was your reason only because it didn't produce or you just wanted to?
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