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Offlineblazedup
Dirk Diggler
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Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Let me pick your brain..BRf cake transfer
    #21299604 - 02/19/15 03:27 PM (9 years, 10 months ago)

I am thinking about cutting a few little chunks off of a colonized cake to either transfer on to agar or lc. At this point I'm just experimenting and learning new tricks.  Any thoughts?

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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
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Re: Let me pick your brain..BRf cake transfer [Re: blazedup]
    #21299612 - 02/19/15 03:29 PM (9 years, 10 months ago)

You'd have to birth in an SAB or rip the cake apart.

I did this and got my PR culture:

Quote:

taGyo said:
Experiments


12/4/14

So I recently finished my first cake. It didn't produce what I wanted because when I started this project I wasn't as knowledgable as I am now. Nevertheless and for the sake of science (and :headbanger:) I decided to try cutting open my cake to look inside of it and see if I could visually see any contaminations. My first PC Puerto Rico cake had a bad bacteria infection and his brothers and sisters got mold before they were even birthed.
So I got my stuff together:



Prepped my hands, picked up the cake and moved it to my SAB:



Flame sterilized my knife, cooled it on a spare agar plate and cut the cake in half carefully:



After that I took the half that didn't have a stroma bubble coming out of it and cut into it until I found this:



Took a sample from that. Skinned where I saw yellow contams and found cake underneath it, nothing to write home about. Started peeling off the verm to get a better look at the actual cake and I could see white, semi-healthy myc underneath it.

Cool, :super:, now for the hard part.

This bubble is a contamination:



I saved this side for last so I could get used to using my knife. The contam grew on both sides but after I cut them all this one resurfaced. At one point I thought I had aerial myc but it turns out it was a reaction to the bacteria. This cake suffered :[. So I carefully took that blotch off and peeled away the verm. Nothing unusual. Started cutting very precise layers so I could get a good look inside.
Nothing.
FInally I took a piece of the now skinned down and broken up cake and cracked it in half:



Cracked it again, sterilized my knife and cut a piece out for a second tissue sample:



Final Pic:



I did a bunch of agar plates and ended last night at 4 am :yawn:,
Up at 9. More agar to make tonight :super:

UPDATE 1:

Both grew out. One got this ugly contam:



Transfered away on 12/11 so we'll see how these two grow.

I think this might be the final transfer boys:




--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: Let me pick your brain..BRf cake transfer [Re: taGyo]
    #21299679 - 02/19/15 03:41 PM (9 years, 10 months ago)

Quote:

taGyo said:
You'd have to birth in an SAB or rip the cake apart.

I did this and got my PR culture:

Quote:

taGyo said:
Experiments


12/4/14

So I recently finished my first cake. It didn't produce what I wanted because when I started this project I wasn't as knowledgable as I am now. Nevertheless and for the sake of science (and :headbanger:) I decided to try cutting open my cake to look inside of it and see if I could visually see any contaminations. My first PC Puerto Rico cake had a bad bacteria infection and his brothers and sisters got mold before they were even birthed.
So I got my stuff together:



Prepped my hands, picked up the cake and moved it to my SAB:



Flame sterilized my knife, cooled it on a spare agar plate and cut the cake in half carefully:



After that I took the half that didn't have a stroma bubble coming out of it and cut into it until I found this:



Took a sample from that. Skinned where I saw yellow contams and found cake underneath it, nothing to write home about. Started peeling off the verm to get a better look at the actual cake and I could see white, semi-healthy myc underneath it.

Cool, :super:, now for the hard part.

This bubble is a contamination:



I saved this side for last so I could get used to using my knife. The contam grew on both sides but after I cut them all this one resurfaced. At one point I thought I had aerial myc but it turns out it was a reaction to the bacteria. This cake suffered :[. So I carefully took that blotch off and peeled away the verm. Nothing unusual. Started cutting very precise layers so I could get a good look inside.
Nothing.
FInally I took a piece of the now skinned down and broken up cake and cracked it in half:



Cracked it again, sterilized my knife and cut a piece out for a second tissue sample:



Final Pic:



I did a bunch of agar plates and ended last night at 4 am :yawn:,
Up at 9. More agar to make tonight :super:

UPDATE 1:

Both grew out. One got this ugly contam:



Transfered away on 12/11 so we'll see how these two grow.

I think this might be the final transfer boys:








Nice work...why not attempt to use a small piece instead of dissecting the whole cake? Was your reason only because it didn't produce or you just wanted to?

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