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OfflineBray
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Registered: 06/23/14
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3 compartment petris
    #21296322 - 02/18/15 10:57 PM (9 years, 10 months ago)

Found a 500 pack of 3 section petris for $91.17. Good price our keep looking?

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OfflineDilated
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Registered: 03/28/14
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Re: 3 compartment petris [Re: Bray]
    #21296339 - 02/18/15 11:03 PM (9 years, 10 months ago)

Quote:

Bray said:
Found a 500 pack of 3 section petris for $91.17. Good price our keep looking?




That sounds like a pretty decent price for that many.  I'm not a big fan of the tri-sectioned dishes however.  I only use them for the inital clone then I transfer to to the basic dish.

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OfflineBray
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Re: 3 compartment petris [Re: Dilated]
    #21296369 - 02/18/15 11:13 PM (9 years, 10 months ago)

I've got several varieties that I'm trying to get a better culture of, went through 50 single section plates in 2 weeks. Figured I've got to save the money.

Edited by Bray (02/18/15 11:13 PM)

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OfflineDilated
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Re: 3 compartment petris [Re: Bray]
    #21296380 - 02/18/15 11:18 PM (9 years, 10 months ago)

Quote:

Bray said:
I've got several varieties that I'm trying to get a better culture of, went through 50 single section plates in 2 weeks. Figured I've got to save the money.




Yeah I've been there too, it can get out of hand sometimes.  Good luck to you though, 3 sections sounds like something you could really use.  I just like that you can track growth for longer on a single dish.  Also sometimes it's hard to spot rhizomorphic growth on 3 sectioned dishes.  Mycelium tends to be tomatose for about a half inch of growth before it starts to thread out rhizomorphically.

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OfflineBray
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Re: 3 compartment petris [Re: Dilated]
    #21296537 - 02/19/15 12:19 AM (9 years, 10 months ago)

I've noticed that I'm waiting a little bit longer to see the rhizomorphic growth, especially on my 3 stone producers. On the other hand, I've got a couple of edibles that start off rhizo and fully colonize a plate in no time at all. All in all, I'm fairly pleased considering these cultures are my first venture into agar.

I'm still uncertain whether I want to take any of the varieties all the way to an isolate. It doesn't seem like I have anything with outstanding genetics, so I'll probably just do a few transfers on each to get some of the fastest growth and then grow them out and hope for something special in the next round.

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Invisibleblindingleaf
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Re: 3 compartment petris [Re: Bray]
    #21296877 - 02/19/15 04:04 AM (9 years, 10 months ago)

i agree with dilated.  i also am not a fan of the Y plates.  it doesn't give the culture as much room to spread out and sector late in the game.  its also a little messier to pour into 3 compartments.  i just put 2-3 wedges on a non sectioned dish if i have to.


stone producers in my experience, do not have the rhizomorphic traits that cubensis does.
i would not isolate cubensis from spores intentionally.  noc up grains with any clean growth you currently have, then spawn to bulk and clone from a nice cluster.  alternatively, let the plate colonize, and once it pins, take a pin to another plate.
isolating from spore can take time, many many plates, and lots of trial and error.  its main advantage is how young the culture is once its selected, vs. a clone that has aged quite a bit more.
in either case, you will need to test performance, so to me, cloning is a quicker way to get a good performer.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflineDilated
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Re: 3 compartment petris [Re: blindingleaf]
    #21297318 - 02/19/15 07:43 AM (9 years, 10 months ago)

Quote:

blindingleaf said:
i agree with dilated.  i also am not a fan of the Y plates.  it doesn't give the culture as much room to spread out and sector late in the game.  its also a little messier to pour into 3 compartments.  i just put 2-3 wedges on a non sectioned dish if i have to.


stone producers in my experience, do not have the rhizomorphic traits that cubensis does.
i would not isolate cubensis from spores intentionally.  noc up grains with any clean growth you currently have, then spawn to bulk and clone from a nice cluster.  alternatively, let the plate colonize, and once it pins, take a pin to another plate.
isolating from spore can take time, many many plates, and lots of trial and error.  its main advantage is how young the culture is once its selected, vs. a clone that has aged quite a bit more.
in either case, you will need to test performance, so to me, cloning is a quicker way to get a good performer.




I couldn't have said it better myself.  I like a big plain plate to allow my to see the "late game" sectoring as BL would call it.  I get paranoid enough dealing with tomatose cultures so I add minimal nutrients to my agar recipe (about 9 parts malt to 10 parts agar agar).  I'm definitely staying away from yeast next go around too, as everything I transferred to it refused to show sectoring.  I've never had my galindoi (stone producer) go rhizomorphic.  It's growth is normally pretty consistent around the edges.

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InvisibleHashish
Knowledge is Power
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Registered: 12/04/14
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Re: 3 compartment petris [Re: Dilated]
    #21297850 - 02/19/15 09:44 AM (9 years, 10 months ago)

im definetly  on the boat for full petris. i have the Y petris also, but agree as they just dont have enough room in each section.

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Invisiblefilthyknees
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Re: 3 compartment petris [Re: Hashish]
    #21298036 - 02/19/15 10:21 AM (9 years, 10 months ago)

I think the point of the sectioned petris are for growing out to make further transfers once the fastest growth is visiable. Only the size of a dime or so. Of course the full plates are best for growing out large amounts to inoc. Different ball game. :shrug:

Good price by me OP


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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OfflineDilated
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Re: 3 compartment petris [Re: filthyknees]
    #21298597 - 02/19/15 12:10 PM (9 years, 10 months ago)

Quote:

filthyknees said:
I think the point of the sectioned petris are for growing out to make further transfers once the fastest growth is visiable. Only the size of a dime or so. Of course the full plates are best for growing out large amounts to inoc. Different ball game. :shrug:

Good price by me OP




QFT

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OfflineDilated
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Re: 3 compartment petris [Re: Dilated]
    #21298844 - 02/19/15 12:59 PM (9 years, 10 months ago)

Hey BL, how do you feel about bee pollen as a nute base in agar recipe?

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OfflineBray
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Re: 3 compartment petris [Re: filthyknees]
    #21298850 - 02/19/15 01:00 PM (9 years, 10 months ago)

So maybe I'll just pick up a couple hundred 3 section plates and order a case of regular plates. That should be plenty for quick transfers and enough standard plates to last a while. Thanks for all of the tips!

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Invisibleblindingleaf
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Re: 3 compartment petris [Re: Bray]
    #21298892 - 02/19/15 01:06 PM (9 years, 10 months ago)

never looked into pollen for agar/other
whats the supposed benefit of adding it?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflineDilated
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Re: 3 compartment petris [Re: blindingleaf]
    #21298910 - 02/19/15 01:09 PM (9 years, 10 months ago)

Quote:

blindingleaf said:
never looked into pollen for agar/other
whats the supposed benefit of adding it?




Not an additive but a replacement for like LME or PD, nutrient replacement.  I ask because I have a bunch of it.

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Invisibleblindingleaf
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Re: 3 compartment petris [Re: Dilated]
    #21298924 - 02/19/15 01:11 PM (9 years, 10 months ago)

oh word.
i got nothing!
let us know though!
what is bee pollen used for in humans?  allergies?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflinetaGyo
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Re: 3 compartment petris [Re: blindingleaf]
    #21298938 - 02/19/15 01:14 PM (9 years, 10 months ago)

I love tri-petris:



:dawerp:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Invisibleblindingleaf
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Re: 3 compartment petris [Re: taGyo]
    #21298966 - 02/19/15 01:18 PM (9 years, 10 months ago)

:poke:
:fishslap:
just kidding tag, u know i love u bro-ski!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflinetaGyo
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Re: 3 compartment petris [Re: blindingleaf]
    #21298979 - 02/19/15 01:20 PM (9 years, 10 months ago)

:paranoid:

:lol:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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