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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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First time wedge use
#21295465 - 02/18/15 08:06 PM (9 years, 10 months ago) |
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I received a wedge and the little vial it came in opened in the process of being mailed to me. I needed to make some agar dishes quickly in case it were to dry out. Now I thought to myself maybe I should make the best out of this chance. So I got everything ready and pLaced them in my SAB. I sterilized the knife I used and sliced the wedge into 3 pieces and placed them onto the agar. All 3 of them seem to be starting to show signs of life. I was looking for illustrated logs of wedge to agar so I would know what I should see and shouldn't see. I wasn't lucky enough to come across what exactly I was looking for. So let me pick your brains plz..
What should I be looking for?
What's the next step?
Did I screw up by cutting it up?
Thanks
Edited by blazedup (02/20/15 09:01 AM)
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: First time wedge use [Re: blazedup]
#21295520 - 02/18/15 08:17 PM (9 years, 10 months ago) |
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You should be looking for healthy growth coming from the wedge. You definitely did NOT screw it up by cutting it with your sterilized blade, and placing a piece of it to several plates was a good idea. If this was a culture sent to you, chances are pretty high that it was a decent one, so trying to isolate would be a mistake. Just wait until it's spread enough that you can transfer to make absolutely sure it's clean, and then transfer from the second plate into a jar of grain.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: Psilicon]
#21295543 - 02/18/15 08:22 PM (9 years, 10 months ago) |
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Quote:
van der griegen said: You should be looking for healthy growth coming from the wedge. You definitely did NOT screw it up by cutting it with your sterilized blade, and placing a piece of it to several plates was a good idea. If this was a culture sent to you, chances are pretty high that it was a decent one, so trying to isolate would be a mistake. Just wait until it's spread enough that you can transfer to make absolutely sure it's clean, and then transfer from the second plate into a jar of grain. 
Thanks for the quick response was kinda nervous I may have made a mistake.
When looking for healthy growth...is it supposed to look like it has white fuzz growing around it?
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Psilicon
Really Nice Guy


Registered: 08/26/12
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Re: First time wedge use [Re: blazedup]
#21295563 - 02/18/15 08:26 PM (9 years, 10 months ago) |
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Yep. Check out Stro's cloning and isolation tek for a lot of pics on what it should look like.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: Psilicon]
#21295657 - 02/18/15 08:42 PM (9 years, 10 months ago) |
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Quote:
van der griegen said: Yep. Check out Stro's cloning and isolation tek for a lot of pics on what it should look like.
Nice..love the complete detail of that tek. I bypassed the clone picture which look similar to how mine are taking off.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: First time wedge use [Re: blazedup]
#21295663 - 02/18/15 08:43 PM (9 years, 10 months ago) |
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Yeah, Stro writes a really good tek. I wish he came around more.
A lot of people call that stage "leaping off," FYI.
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AbdouRetro
Shroomer in Grime


Registered: 11/01/11
Posts: 69
Last seen: 7 years, 11 months
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Re: First time wedge use [Re: Psilicon]
#21295675 - 02/18/15 08:45 PM (9 years, 10 months ago) |
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that was the right thing to do.. are you wrong.. of fucking course not!
idiot customs people like to shove everything up their asses for no reason and ruin cultures all the time.. that's why I order spore prints from seed rings.. cheap and safe.
hopefully its already isolated.. which will mean that it will all grow as a single circle evenly .. check RR's videos about agar.
in your case you might just need to isolate it from whatever else grows on the agar... think the mailman's anal flora..
onto a clean jar then onto an LC and substrate that you will G2G for ideal results (Y)
happy shrooming
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: AbdouRetro]
#21296966 - 02/19/15 05:21 AM (9 years, 10 months ago) |
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Quote:
AbdouRetro said: that was the right thing to do.. are you wrong.. of fucking course not!
idiot customs people like to shove everything up their asses for no reason and ruin cultures all the time.. that's why I order spore prints from seed rings.. cheap and safe.
hopefully its already isolated.. which will mean that it will all grow as a single circle evenly .. check RR's videos about agar.
in your case you might just need to isolate it from whatever else grows on the agar... think the mailman's anal flora..
onto a clean jar then onto an LC and substrate that you will G2G for ideal results (Y)
happy shrooming 
What's a seed ring?
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: blazedup]
#21296971 - 02/19/15 05:23 AM (9 years, 10 months ago) |
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If they grow correctly with no contams would it make sense to make an LC? I would like to possibly make some syringes out of it
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: First time wedge use [Re: blazedup]
#21296976 - 02/19/15 05:26 AM (9 years, 10 months ago) |
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agar is the best way to make an LC, so yes. but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Quote:
blindingleaf said: agar is the best way to make an LC, so yes. but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.
I was think the as well. If possible use one of the 3 for LC and another for grain. The third I would put on a master slant to preserve for future use.
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 11 months, 12 days
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Quote:
blindingleaf said: agar is the best way to make an LC, so yes. but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.
I second this idea.
I've been very impressed with my recent agar wedge into grain growth rates.
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AbdouRetro
Shroomer in Grime


Registered: 11/01/11
Posts: 69
Last seen: 7 years, 11 months
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Re: First time wedge use [Re: blazedup]
#21300399 - 02/19/15 06:35 PM (9 years, 10 months ago) |
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a seed ring is a place the helps circulate mushroom prints for a small fee unlike commercial sellers. its done in a way to spread the "magic and divinity" behind the psychedelic experiences without financial gain.. at least that's what I understand..
here are ones that are tried and tested
http://fsre.nl/
http://freespore.com/index.html
they are rather slow though... but they do work.. also the risk is very little.. so don't think about them much.. but you will be happy when their prints show up
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: AbdouRetro]
#21300642 - 02/19/15 07:24 PM (9 years, 10 months ago) |
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Not the greatest pic but just wanted to show an update of my progress.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: blazedup]
#21307028 - 02/21/15 07:17 AM (9 years, 10 months ago) |
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Still can't get my camera to focus like it should
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: blazedup]
#21307256 - 02/21/15 08:50 AM (9 years, 10 months ago) |
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Any suggestions when I can take a wedge from this and put it in lc or grain?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: First time wedge use [Re: blazedup]
#21307261 - 02/21/15 08:51 AM (9 years, 10 months ago) |
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LC any time,
Grain I'd wait for it to get a little bigger then that.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: First time wedge use [Re: taGyo]
#21307270 - 02/21/15 08:53 AM (9 years, 10 months ago) |
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Quote:
taGyo said: LC any time,
Grain I'd wait for it to get a little bigger then that.
If transferring to lc should I just take a small wedge of new growth and leave the original wedge to continue to grow?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
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Re: First time wedge use [Re: blazedup]
#21307272 - 02/21/15 08:54 AM (9 years, 10 months ago) |
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Yup.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
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Re: First time wedge use [Re: taGyo]
#21307276 - 02/21/15 08:55 AM (9 years, 10 months ago) |
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Glad to see it survived the trip. Good luck, man!
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