Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
First time wedge use
    #21295465 - 02/18/15 08:06 PM (9 years, 10 months ago)

I received a wedge and the little vial it came in opened in the process of being mailed to me. I needed to make some agar dishes quickly in case it were to dry out. Now I thought to myself maybe I should make the best out of this chance. So I got everything ready and pLaced them in my SAB. I sterilized the knife I used and sliced the wedge into 3 pieces and placed them onto the agar. All 3 of them seem to be starting to show signs of life. I was looking for illustrated logs of wedge to agar so I would know what I should see and shouldn't see. I wasn't lucky enough to come across what exactly I was looking for. So let me pick your brains plz..

What should I be looking for?

What's the next step?

Did I screw up by cutting it up?



Thanks

Edited by blazedup (02/20/15 09:01 AM)

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: First time wedge use [Re: blazedup]
    #21295520 - 02/18/15 08:17 PM (9 years, 10 months ago)

You should be looking for healthy growth coming from the wedge.  You definitely did NOT screw it up by cutting it with your sterilized blade, and placing a piece of it to several plates was a good idea.  If this was a culture sent to you, chances are pretty high that it was a decent one, so trying to isolate would be a mistake.  Just wait until it's spread enough that you can transfer to make absolutely sure it's clean, and then transfer from the second plate into a jar of grain.  :smile:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: Psilicon]
    #21295543 - 02/18/15 08:22 PM (9 years, 10 months ago)

Quote:

van der griegen said:
You should be looking for healthy growth coming from the wedge.  You definitely did NOT screw it up by cutting it with your sterilized blade, and placing a piece of it to several plates was a good idea.  If this was a culture sent to you, chances are pretty high that it was a decent one, so trying to isolate would be a mistake.  Just wait until it's spread enough that you can transfer to make absolutely sure it's clean, and then transfer from the second plate into a jar of grain.  :smile:




Thanks for the quick response was kinda nervous I may have made a mistake.

When looking for healthy growth...is it supposed to look like it has white fuzz growing around it?

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: First time wedge use [Re: blazedup]
    #21295563 - 02/18/15 08:26 PM (9 years, 10 months ago)

Yep.  Check out Stro's cloning and isolation tek for a lot of pics on what it should look like.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: Psilicon]
    #21295657 - 02/18/15 08:42 PM (9 years, 10 months ago)

Quote:

van der griegen said:
Yep.  Check out Stro's cloning and isolation tek for a lot of pics on what it should look like.




Nice..love the complete detail of that tek. I bypassed the clone picture which look similar to how mine are taking off.

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: First time wedge use [Re: blazedup]
    #21295663 - 02/18/15 08:43 PM (9 years, 10 months ago)

Yeah, Stro writes a really good tek.  I wish he came around more. 

A lot of people call that stage "leaping off," FYI.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
OfflineAbdouRetro
Shroomer in Grime
 User Gallery

Registered: 11/01/11
Posts: 69
Last seen: 7 years, 11 months
Re: First time wedge use [Re: Psilicon]
    #21295675 - 02/18/15 08:45 PM (9 years, 10 months ago)

that was the right thing to do..
are you wrong.. of fucking course not!

idiot customs people like to shove everything up their asses for no reason and ruin cultures all the time.. that's why I order spore prints from seed rings.. cheap and safe.

hopefully its already isolated.. which will mean that it will all grow as a single circle evenly .. check RR's videos about agar.

in your case you might just need to isolate it from whatever else grows on the agar... think the mailman's anal flora..

onto a clean jar then onto an LC and substrate that you will G2G for ideal results (Y)

happy shrooming :laugh:

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: AbdouRetro]
    #21296966 - 02/19/15 05:21 AM (9 years, 10 months ago)

Quote:

AbdouRetro said:
that was the right thing to do..
are you wrong.. of fucking course not!

idiot customs people like to shove everything up their asses for no reason and ruin cultures all the time.. that's why I order spore prints from seed rings.. cheap and safe.

hopefully its already isolated.. which will mean that it will all grow as a single circle evenly .. check RR's videos about agar.

in your case you might just need to isolate it from whatever else grows on the agar... think the mailman's anal flora..

onto a clean jar then onto an LC and substrate that you will G2G for ideal results (Y)

happy shrooming :laugh:




What's a seed ring?

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: blazedup]
    #21296971 - 02/19/15 05:23 AM (9 years, 10 months ago)

If they grow correctly with no contams would it make sense to make an LC? I would like to possibly make some syringes out of it

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: First time wedge use [Re: blazedup]
    #21296976 - 02/19/15 05:26 AM (9 years, 10 months ago)

agar is the best way to make an LC, so yes.
but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: blindingleaf]
    #21297063 - 02/19/15 06:14 AM (9 years, 10 months ago)

Quote:

blindingleaf said:
agar is the best way to make an LC, so yes.
but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.




I was think the as well. If possible use one of the 3 for LC and another for grain. The third I would put on a master slant to preserve for future use.

Extras: Filter Print Post Top
OfflineCliftonGK1
Sasquatch
Male


Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 11 months, 12 days
Re: First time wedge use [Re: blindingleaf]
    #21297099 - 02/19/15 06:31 AM (9 years, 10 months ago)

Quote:

blindingleaf said:
agar is the best way to make an LC, so yes.
but i would also consider dropping some wedges into grain(s) as well for backup in case the LC fails.




I second this idea.

I've been very impressed with my recent agar wedge into grain growth rates.


--------------------

Extras: Filter Print Post Top
OfflineAbdouRetro
Shroomer in Grime
 User Gallery

Registered: 11/01/11
Posts: 69
Last seen: 7 years, 11 months
Re: First time wedge use [Re: blazedup]
    #21300399 - 02/19/15 06:35 PM (9 years, 10 months ago)

a seed ring is a place the helps circulate mushroom prints for a small fee unlike commercial sellers. its done in a way to spread the "magic and divinity" behind the psychedelic experiences without financial gain..
at least that's what I understand..

here are ones that are tried and tested

http://fsre.nl/

http://freespore.com/index.html


they are rather slow though... but they do work.. also the risk is very little.. so don't think about them much.. but you will be happy when their prints show up :laugh:

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: AbdouRetro]
    #21300642 - 02/19/15 07:24 PM (9 years, 10 months ago)



Not the greatest pic but just wanted to show an update of my progress.

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: blazedup]
    #21307028 - 02/21/15 07:17 AM (9 years, 10 months ago)

Still can't get my camera to focus like it should

]

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: blazedup]
    #21307256 - 02/21/15 08:50 AM (9 years, 10 months ago)

Any suggestions when I can take a wedge from this and put it in lc or grain?

Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 6 years, 3 months
Re: First time wedge use [Re: blazedup]
    #21307261 - 02/21/15 08:51 AM (9 years, 10 months ago)

LC any time,

Grain I'd wait for it to get a little bigger then that.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
Re: First time wedge use [Re: taGyo]
    #21307270 - 02/21/15 08:53 AM (9 years, 10 months ago)

Quote:

taGyo said:
LC any time,

Grain I'd wait for it to get a little bigger then that.




If transferring to lc should I just take a small wedge of new growth and leave the original wedge to continue to grow?

Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 6 years, 3 months
Re: First time wedge use [Re: blazedup]
    #21307272 - 02/21/15 08:54 AM (9 years, 10 months ago)

Yup.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

Extras: Filter Print Post Top
OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 7 years, 5 months
Re: First time wedge use [Re: taGyo]
    #21307276 - 02/21/15 08:55 AM (9 years, 10 months ago)

Glad to see it survived the trip. Good luck, man!


--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: MagicBag.co All-In-One Bags That Don't Suck   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* How does this mycelium (on agar) look? Should I do another transfer? CaptainAhab 1,015 6 10/22/10 11:14 PM
by PrimalSoup
* 2 agar ?s. Best way to transfer to grain jars? & How does my myc. transfer look? (pic) FungiFarmer 14,296 11 09/09/09 02:43 PM
by FungiFarmer
* EvilMushroom's Wedge Tek
( 1 2 3 4 5 6 all )
EvilMushroom666 6,661 116 03/10/11 10:41 AM
by EvilMushroom666
* G2G transfers...an endless cycle? ADoobie 7,510 18 11/12/20 07:10 PM
by FredsGotSlacks
* transferring, what myc to choose? FractalXplora 2,312 15 11/30/10 03:59 AM
by FractalXplora
* Agar to Liquid Culture. How much tissue to transfer? TheFish 1,705 3 02/12/08 10:10 AM
by TheFish
* Can Grain-to-Grain Transfers Be Done Indefinetly? LogicaL ChaosM 15,836 13 04/08/24 11:18 AM
by Kehfrey
* transferring mycelium wikedanjel 4,251 13 01/10/03 03:54 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,195 topic views. 18 members, 94 guests and 70 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.004 seconds on 14 queries.