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PinPornProducer
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Registered: 08/23/14
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Re: If light is so important [Re: Kizzle]
#21289163 - 02/17/15 03:23 PM (9 years, 10 months ago) |
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Another thing I noticed about growing in the dark is that contams become almost non exsistant. The tub I grew in the dark flushed 8 times before just laying dormant. Never went bad, just flushed till it died. That's the longest flushing tub I've had and just so happens to be the one fruited in the dark
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cronicr



Registered: 08/07/11
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Quote:
PinPornProducer said: Another thing I noticed about growing in the dark is that contams become almost non exsistant. The tub I grew in the dark flushed 8 times before just laying dormant. Never went bad, just flushed till it died. That's the longest flushing tub I've had and just so happens to be the one fruited in the dark
shit goes moldy in my fridge if i leave town for a couple weeks light has little to do with contams
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PinPornProducer
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Re: If light is so important [Re: cronicr]
#21289193 - 02/17/15 03:29 PM (9 years, 10 months ago) |
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Cheese and living mycelium are 2 different things
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cronicr



Registered: 08/07/11
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i still don't see why ya think no light would equal no mold seems silly to me, jars were going moldy way back when colonizing in the dark was the norm
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
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amp244
Sporocarp Stretching


Registered: 08/05/08
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Ok so the actual abstract from the PDF file is different than the one I posted earlier. This is more clear and concise.
Abstract Different variables including species, strain, glucose and ammonium succinate concentration in the growth medium, pH, temperature, timing and oxidation have been accounted for the Psilocin (PC) and Psilocybin (PB) content in the "Magic Mushrooms"(MMs). These are but some of the variables in a constellation of factors that complicate consistency in the production of PC and PB. In an attempt to study the effect of light on chemical constitutions, some samples were kept in dark, some samples were kept in dim lighting, whereas others were exposed to natural but indirect light. After picking and drying Mushrooms, a simple one-step extraction involving homogenization of the dried fruit bodies of fungi in chloroform and derivatization with MSTFA was performed. The samples were then analyzed by gas chromatography–mass spectrometry. This investigation shows that the relative PC and PB content of the mushrooms is highly dependent on the light condition. This variation could amount to 100 fold of active components (PB and PC) in samples harvested in dark condition compared to the samples harvested in indirect light condition. Therefore it can be concluded that UV light may have destructive effect on the active components, which would readily explain why sun-struck collections are less potent.
-------------------- How to Convert a Normal 24-hour Light Timer into a Short Cycle Repeating Timer
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
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Quote:
PinPornProducer said: Another thing I noticed about growing in the dark is that contams become almost non exsistant.
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cronicr



Registered: 08/07/11
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Re: If light is so important [Re: Mr. Alien]
#21289213 - 02/17/15 03:35 PM (9 years, 10 months ago) |
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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PinPornProducer
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Re: If light is so important [Re: cronicr]
#21289226 - 02/17/15 03:37 PM (9 years, 10 months ago) |
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Poor sterile technique causing a contam to an uncolonised jar of inoculated grains is different than a healthy substrate subcoming to trich halfway through it's life span
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cronicr



Registered: 08/07/11
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Quote:
PinPornProducer said: Poor sterile technique causing a contam to an uncolonised jar of inoculated grains is different than a healthy substrate subcoming to trich halfway through it's life span
you're making no sense at all, a healthy sub is contam resistent on it's own and it has nothing to do with light lol
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krypto2000
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Re: If light is so important [Re: mustangbob3]
#21289235 - 02/17/15 03:40 PM (9 years, 10 months ago) |
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Quote:
mustangbob3 said:
Quote:
I just put two mono's under a 600w hps and ~300w worth of LED's. 
if the distance of lights is equal then i would say it is agood experiment!!
if i had to guess based on info gleaned in this thread alone, i would say it is likely the one under hps(higher intensity) wont grow as tall but will be more dense and they will mature and open the caps earlier. i wonder about weight difference too as early maturation may equal less weight but they may make up for it by being more dense?
please post results!! very interesting to see.
I will keep you updated. It's not two separate experiments, they're both under an HPS & two LED's. I just had some spare room in my grow tent so I stuck em in there to see how they'd perform. It won't be a perfect test because the lights will raise the temperature a good 10 degrees or so and I'm counting on that heat and the light dramatically increasing the rate of evaporation so there are other factors that will change besides just the light. I'm curious how it will turn out though. I imagine a metal halide would be better than an HPS, but an HPS still has a lot of usable spectrum for the mushrooms. Either way I imagine they're receiving more light than they can make use of, so if there is such a thing as too much light this experiment should definitely show it.
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Kizzle
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Re: If light is so important [Re: cronicr]
#21289313 - 02/17/15 04:02 PM (9 years, 10 months ago) |
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Quote:
cronicr said: i still don't see why ya think no light would equal no mold seems silly to me, jars were going moldy way back when colonizing in the dark was the norm
It's well known that light causes Trichoderma to sporulate. So no mold could mean no visible mold, hence no throwing out your substrate at the sight of it.
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Mr. Alien
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Re: If light is so important [Re: Kizzle]
#21289335 - 02/17/15 04:07 PM (9 years, 10 months ago) |
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So if there is no light.. trich can't sporulate?
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cronicr



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Re: If light is so important [Re: Kizzle]
#21289462 - 02/17/15 04:41 PM (9 years, 10 months ago) |
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Quote:
Kizzle said:
Quote:
cronicr said: i still don't see why ya think no light would equal no mold seems silly to me, jars were going moldy way back when colonizing in the dark was the norm
It's well known that light causes Trichoderma to sporulate. So no mold could mean no visible mold, hence no throwing out your substrate at the sight of it. 
trich is one of many enemies
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Kizzle
Misanthrope


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Re: If light is so important [Re: Mr. Alien]
#21289472 - 02/17/15 04:44 PM (9 years, 10 months ago) |
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It will eventually regardless but it has a huge effect. If you've ever seen a Trichoderma culture like this those rings of spores are formed on the outside of the colony only during the time it's exposed to light.
 I think most would agree that in general noticeable Trich growth usually doesn't appear until exposed to fruiting conditions which for almost everyone includes light. I have noticed before the thick white mycelium appearing in opaque trays with no color change for at least a week without turning green, I didn't keep it any longer than that as the smell of Trich was very obvious. Under normal conditions it would happen only a couple days after it's appearance.
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blindingleaf
blue collar underworld


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Re: If light is so important [Re: Kizzle]
#21289566 - 02/17/15 05:04 PM (9 years, 10 months ago) |
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nice addition kizzle! that plate is awesome (in its own way i mean...)
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cronicr



Registered: 08/07/11
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awesome pic
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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amp244
Sporocarp Stretching


Registered: 08/05/08
Posts: 1,336
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Re: If light is so important [Re: cronicr]
#21290017 - 02/17/15 06:40 PM (9 years, 10 months ago) |
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So I read that article about the effect of light on potency. The article was interesting but I am a bit apprehensive about accepting it as truth. However, it did cite some interesting articles that were much more professional in nature.
The text has a few grammatical errors, which is a little disconcerting, but that could be due to the fact that the authors are Iranian. Also, a good portion of the text is copied verbatim from the sources cited. I don’t know if this is an acceptable practice in the scientific community, and although cited, seems unprofessional to me.
Also, the “Sample Preparation” section seems way too short for me. They speak of temperature, humidity, and light, but they say nothing about the techniques used. Nor do they mention CO2 concentrations or air exchanges. There is simply not enough information to validate the statistical significance of the results. Too many variables that need to be constants regarding the fructification of the tested specimens were omitted from description in the “Sample Preparation”. Perhaps I am being too stringent, but I feel as if those details are important.
And then there are the results. I understand that the Psilocybin is dephosphorylated into psilocin by heating the MSTFA derivative. They then say that the psilocin/psilocybin content “can then be calculated using the AUC as an indirect index of the content”. So they are using statistics to guess the psilocybin content based on the detected psilocin content? Am I understanding that correctly? And regardless of their methods to determine Psilocin/psilocybin content, I lack the statistical competence required to comprehend the tables and charts illustrating the results, so I’m S.O.L either way. And that is certainly no problem of the authors'.
All in all the basic conclusion is that they believe the results indicate that ultraviolet radiation from the sun lessens the potency of fruit bodies, which doesn’t seem too farfetched to me.
Then I read this little statement: “Different variables including species, strain, glucose and ammonium succinate concentration in the growth medium, pH, temperature, timing and oxidation have been accounted for the PC and PB content in the "Magic Mushrooms"(MMs). For example oxidation, absence of glucose and low levels of ammonium succinate will all give poor yields of PC/PB production. Whereas, adjustment of pH, temperature and timing reported to increase the PC/PB content”. This prompted me to look up the source, which yielded this pdf which is completely off topic but very interesting: https://www.erowid.org/archive/rhodium/pdf/shroom.psilocin.extraction.pdf
You could go off on a serious "youtube-esque" tangent checking all these sources but damn is it interesting. Its one of those, “How far down the rabbit hole do you want to go” type situations.
-------------------- How to Convert a Normal 24-hour Light Timer into a Short Cycle Repeating Timer
"Monopoly, besides, is a great enemy to good management, which can never be universally established but in consequence of that free and universal competition which forces everybody to have recourse in it for the sake of self-defense." -Adam Smith
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PinPornProducer
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Re: If light is so important [Re: amp244]
#21291331 - 02/17/15 11:16 PM (9 years, 10 months ago) |
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Found this from RR
Last, but not by any means least is exposure to light. Light does much more than just tell the mushrooms which way to grow. There are mechanisms in the light that stimulate the formation of hyphal knots as well, and light at the higher end of the spectrum(blue) definitely, absolutely stimulate more hyphal knots(which grow into primordia, which then morph into pins) than light at the lower end of the spectrum(red) This does not mean to get a 'mood light' with a blue lens, but rather to select lights such as metal halide, or much more economical is 'natural daylight' fluorescent that emit light at around 6,000 kelvin to 7,500 kelvin depending on the brand. Cool white fluorescent emit light at around 5,000 kelvin and the 'red' incandescents emit light at around 3,000 kelvin. The higher the light temperature in kelvin, the more stimulatory it is to hyphal knot formation. I hope this helps. RR
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PinPornProducer
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Naf1 Foundress Queen ***** Posts: 753
Re: Increasing Alkaloid Content of Liquid Psilocybe Cubensis Mycelium Culture « Reply #9 on: June 13, 2010, 05:57:04 AM » Light is not really relevant as the mycellium do not need any they are usually under the ground (they do not photosynthesize they are saprophages and their food has been supplied by you), they only need around 1/100 hundredth of a second of light to trigger fruiting as they are actually photosensitive and will produce a brown pigment in the cap when exposed to light. Also to get the bastards to knot the substrate has to be fully (100%)colonized, so they are not going to knot (unless one or two fruit on the surface, but just keep shaking it). You never know though? But would imagine UV could eventually break down psilocybin lowering yields, but could also trigger alkaloid production
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PinPornProducer
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What I'm gathering from these 2 statements is coinciding with my findings. Colonize and pin under extreme lighting for a nice pinset and then greatly reduce or even remove lighting during fruiting for denser more potent fruits
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