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Offlineblazedup
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LC question..and then some
    #21288035 - 02/17/15 01:27 PM (9 years, 10 months ago)

So I been reading and reading and reading about LC. I came up with a few questions and maybe someone can help out and not try telling me to use the search engine. I have already read that LC to LC is not smartest thing to do. Also a lot of people don't like LC all together and prefer agar work. I am about to get a syringe and figuring out how to duplicate so I can share.

If theory can someone take any LC and eventually clean it up or isolate on agar because it's live?

If you have good growth on agar, is the contam concern still the same placing a wedge in LC jar?

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InvisibleMunchauzen
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Re: LC question..and then some [Re: blazedup]
    #21288111 - 02/17/15 01:49 PM (9 years, 10 months ago)

LCs are fine if you start them with a clean piece of agar and yes you can clean up an LC on agar. Starting an LC with a clean LI is also good.

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OfflinehamloafM
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Re: LC question..and then some [Re: blazedup]
    #21288283 - 02/17/15 02:07 PM (9 years, 10 months ago)

Quote:

blazedup said:

If you have good growth on agar, is the contam concern still the same placing a wedge in LC jar?



Yes.  You don't ever want to take a piece of clean culture directly from a contaminated culture and inoculate a LC with it.

Before LC's can be inoculated with a dirty culture the culture must first be transferred away from the contamination into new, receiving agar mediums.  The aforementioned procedure should be repeated until a culture free of contamination is generated.  The amount of transfers required to generate a clean culture will vary.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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