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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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Offlinepharmacist
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Registered: 10/12/03
Posts: 19
Last seen: 12 years, 10 months
Not colonizing
    #2128516 - 11/22/03 01:03 AM (13 years, 21 days ago)

this is my first attempt at growing and 3 of my 12 jars aren't colonizing. Is there anything that I can do to get them started?
Thanks


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OfflineMycoCat
a.k.a. ShroomCat

Registered: 10/09/03
Posts: 1,042
Last seen: 2 years, 11 months
Re: Not colonizing [Re: pharmacist]
    #2128517 - 11/22/03 01:04 AM (13 years, 21 days ago)

How long has it been? What substrate are you using? How big are the jars? Are your inoculation points covered by tape?


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No question is so difficult to answer as that to which the answer is obvious.

Meow.


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Offlinepharmacist
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Registered: 10/12/03
Posts: 19
Last seen: 12 years, 10 months
Re: Not colonizing [Re: MycoCat]
    #2128518 - 11/22/03 01:06 AM (13 years, 21 days ago)

16 days, brown rice flour and verm, 1/2 pint, no.


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OfflineMycoCat
a.k.a. ShroomCat

Registered: 10/09/03
Posts: 1,042
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Re: Not colonizing [Re: pharmacist]
    #2128519 - 11/22/03 01:08 AM (13 years, 21 days ago)

Patience. I don't know why they wouldn't be colonizing after that long.

How far along are the other jars?


--------------------
No question is so difficult to answer as that to which the answer is obvious.

Meow.


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Offlinepharmacist
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Registered: 10/12/03
Posts: 19
Last seen: 12 years, 10 months
Re: Not colonizing [Re: MycoCat]
    #2128520 - 11/22/03 01:08 AM (13 years, 21 days ago)

About 80 percent. a few are closer to 100%.


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InvisibleCloud9
I don't feel, and it feels great
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Posts: 1,548
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Re: Not colonizing [Re: pharmacist]
    #2128521 - 11/22/03 01:14 AM (13 years, 21 days ago)

You could have not waited long enough and they could have been too hot still when you innoculated.. Or the spores may have simply not been enough (too diluted) to germinate the jars. Usualy I found the reason most people have problems with growth is they are too eager and inject when it is still too hot and they kill the spores. Then again i've seen spores start germinating after 3 weeks to 5 weeks before..


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OfflineMycoCat
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Re: Not colonizing [Re: Cloud9]
    #2128527 - 11/22/03 01:17 AM (13 years, 21 days ago)

That wouldn't make sense for him though...if nine of his jars are growing that probably isn't the problem.

I would honestly say wait. Cloud9 is right, spores can suddenly pop up after extremely long periods of time.


--------------------
No question is so difficult to answer as that to which the answer is obvious.

Meow.


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Offlinejong21
Mycologist/CSMajor

Registered: 05/28/03
Posts: 576
Loc: Berkeley, CA
Last seen: 11 years, 9 months
Re: Not colonizing [Re: MycoCat]
    #2128627 - 11/22/03 02:31 AM (13 years, 21 days ago)

well considering it is your first attempt and 9/12 are colonizing, without any further information from you I'd say that isn't too bad...my first attempt I got 2/12 jars to colonize, and only at 1 of the 4 innoculation points so it took like 45 days to fully colonize. you'll get better w/ practice, no worries, but more info needed to make a judgement now...


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I either talk about my friends in the first or third person, but I never, ever talk about myself on this website. Except that last sentence.


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InvisibleCloud9
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Re: Not colonizing [Re: jong21]
    #2128628 - 11/22/03 02:34 AM (13 years, 21 days ago)

My friends first try he had all 12 colonize but 2 got contaminated. and the ones that were ok also only colonized at 1 point per jar so it took 6 weeks for them to fully colonize...


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Anonymous

Re: Not colonizing [Re: pharmacist]
    #2128649 - 11/22/03 02:50 AM (13 years, 21 days ago)

there may not have been enough spores in there. if your syringe was too dilute (where'd you get it?) or you didn't use enough (how much did you use?) that could be the problem... the other possibility is that some kind of bacterial contamination whacked those jars. in that case they'd probably smell pretty funk. every time i've ever gotten bacteria it's been a sortof sick sweet smell.


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InvisibleDatDaNK420
SeriousShroomage
Registered: 06/23/03
Posts: 612
Re: Not colonizing [Re: ]
    #2128681 - 11/22/03 03:30 AM (13 years, 21 days ago)

I had one jar out of 20 not colonizing at all like all the others, so i knocked it back up and tada!!


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InvisibleHanky
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Re: Not colonizing [Re: DatDaNK420]
    #2128810 - 11/22/03 08:13 AM (13 years, 21 days ago)

you can be happy that 9 jars are colonising.


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Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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OfflineTheDarkSideof_Paco
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Registered: 11/10/03
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Re: Not colonizing [Re: Hanky]
    #2128938 - 11/22/03 11:36 AM (13 years, 21 days ago)

did you shake your syringe after each jar?


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"In order to rally people, governments need enemies. They want us to be afraid, to hate, so we will rally behind them. And if they do not have a real enemy, they will invent one in order to mobilize us."

Thich Nhat Hanh


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Offline95rh
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Registered: 05/03/03
Posts: 115
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Re: Not colonizing [Re: TheDarkSideof_Paco]
    #2129335 - 11/22/03 05:57 PM (13 years, 20 days ago)

Tempeture seems to be the main factor slowing growth down. for cubie's keep it at 80-85 F and you're golden. Personally I prefer 80 since the jars generate thier own heat and will be a few degrees warmer than the rest of the incubator. By far the best hunidifier's are the double rubbermaid containers with water and fish heater in the bottom one. humidity isn't really a factor here but you don't want anything that will cause the jars to dry out

Could also be water content in the jars to dry and they'll never grow, to wet and everything turns to mud and won't grow. The real trick is getting em to fruit. Once you get your first harvest everything starts to fall into place really well and it gets easier each time.

rh95


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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