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Amazon Shop: ½ Pint Jars, Rye Grain, Scales, Wild Bird Seed

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OfflineDeeBo
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Fractional sterilization Water content
    #2128401 - 11/21/03 11:34 PM (13 years, 7 months ago)

If your heating for consecutive times how do you calculate water content? Won't more water be lost in each heating period. I.e.

-Single sterilization 1 pint = 100g rye/105g water.

-Fractional sterilization 1 pint = 100g rye/??? water.


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Edited by DeeBo (11/21/03 11:42 PM)


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OfflineAnnoA
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Re: Fractional sterilization Water content [Re: DeeBo]
    #2128722 - 11/22/03 04:52 AM (13 years, 7 months ago)

Usually you can use the same amounts. And as always:

" It?s best to make a batch of test jars when one acquires a new grain for instance 100g rye and 100, 105, 110, 115 and 120g water.
Then you?ll see which water content provides the best result."


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OfflineDeeBo
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Re: Fractional sterilization Water content [Re: Anno]
    #2129325 - 11/22/03 05:41 PM (13 years, 7 months ago)

Ok thanks Anno. Gonna do some tests and find the right water amount for sure. Gonna try 110g and 120g think that should be the ones.


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Offlineblackout
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Re: Fractional sterilization Water content [Re: DeeBo]
    #2134315 - 11/25/03 08:34 AM (13 years, 6 months ago)

you could weigh the jars to see how much you have lost


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Re: Fractional sterilization Water content [Re: blackout]
    #2140883 - 11/27/03 09:28 PM (13 years, 6 months ago)

bingo


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Re: Fractional sterilization Water content [Re: blackout]
    #2293462 - 02/02/04 08:32 AM (13 years, 4 months ago)

I am testing this at the moment. added exactly 1kg millet to a litre of water in a bag so it all weighs 2kg. i microwaved it for 30mins. will do 2-3 more times incubating in between for a day or 2. keep boiling until it is 1.75kg then i will add 50ml of liquid innoc to bring it up to the recommended 80ml/100g millet. the more millet the better as it makes the % error in using a household scale less. I could have fit twice that amount in a bag in the microwave, no chance in a household PC.
any forseeable problems??


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OfflineAnnoA
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Re: Fractional sterilization Water content [Re: blackout]
    #2293468 - 02/02/04 08:41 AM (13 years, 4 months ago)

>keep boiling until it is 1.75kg

This could very well result in a thick mush....be careful with additing too much water to grain.


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Offlineblackout
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Re: Fractional sterilization Water content [Re: Anno]
    #2293481 - 02/02/04 08:51 AM (13 years, 4 months ago)

i have already microwaved it with 1 litre and it seems fine. it formed some clumps which were easily mixed back in. when it dries a bit more i would expect hot spots in the microwave will be a problem so i will take it out and shake a bit.


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OfflineGr0wer
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Re: Fractional sterilization Water content [Re: blackout]
    #2294893 - 02/02/04 07:20 PM (13 years, 4 months ago)

When you boil you need to have a lid on the pot. The humidity in the pot will stop most moisture loss. I fractional steralized a bunch of WBS jars, 30mim boil then a day to sit x3, so far 20% colonization no contams and the moisture seems good. I microwaved one jar and it came out way too dry i had to have a layer of water at the bottom to keep the moisture in.


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Re: Fractional sterilization Water content [Re: Gr0wer]
    #2296308 - 02/03/04 04:42 AM (13 years, 4 months ago)

good to hear its going well, keep us informed. the microwave way is very easy and most have a microwave, i dont have a pot big enough for what i can fit in the microwave. i had the bag closed a bit and the pressure blew it up and stretched the bag. i put it in another and just folded the top over. i microwaved it for about 20mins and it is now down to 1.6kg. so i will either have to add more water for the last heating or add lots of liquid innoc


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OfflineGr0wer
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Re: Fractional sterilization Water content [Re: blackout]
    #2297407 - 02/03/04 03:13 PM (13 years, 4 months ago)

You cant heav it sealed, and like i said having a layer of extra water is good. My jars i innoculated with about 1/4 of water at the bottom because i couldnt drain it al out. After some colonization, shaking it spread all the moisture out and now theres no pooling at the bottom anymore. Make sure the water is boiled a bit just in case, though most tap water is sterile.


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Re: Fractional sterilization Water content [Re: Gr0wer]
    #2299613 - 02/04/04 05:51 AM (13 years, 4 months ago)

most tap water wont be sterile but is easily sterilised by boiling. i added 300ml to my bag and microwaved again so it is at 1.85kg. i will microwave down to 1.75 again and then seal the bag and microwave on low power so it blows up just a bit but doesnt stretch. this will make sure the inside is sterile.


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Re: Fractional sterilization Water content [Re: blackout]
    #2418501 - 03/11/04 10:39 AM (13 years, 3 months ago)

my millet finally showed growth about 3 days ago. i innoced with some old B+ spores from a homemade print i got so i wasnt sure if i would get contams. looks good so far not growing too fast though but a few starting points.


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Amazon Shop: ½ Pint Jars, Rye Grain, Scales, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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