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Invisible3iRiS9
@psilocybeing
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Incubation temp question
    #21275476 - 02/15/15 12:14 AM (9 years, 3 months ago)

This is my first grow.

I have 12 PF style jars inoculated with P. cubensis on 2-7.
(To "Let's Grow Mushrooms!" Specs. Thank you RR.)
I live in Florida, so my room temp was a perfect 75˚f give or take.
First growth noticed on 2-11. This is roughly 5 days after inoculation:


My room hasn't been below 70˚f in many moons, but the latest cold front has brought my room temp down to ≤68˚f!

The mycelium looks healthy (20x mag. loupe):


However, it has pretty much stalled since the front passed over us on 2-13.
Somehow I had the foresight to keep track of the colonization zone.
Same Jar, Same inoculation port, current date (2-15):


As you can see, the last outline was taken on 2-13, and it has barely budged.
I'm probably overly worried, but most of my concern is for how long this will last. 
I'm expecting these temps for many more days consecutively.
I know this is less than optimal for incubation, I'm wondering how long they can take this and if I should intervene with a TiT or similar Tek.

Any help is greatly appreciated!
Thank you all for helping me to get this far.
Way to exited to jump into cultivation. :cheers:

Edited by 3iRiS9 (02/17/15 07:45 AM)

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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,346
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21275507 - 02/15/15 12:32 AM (9 years, 3 months ago)

don't worry about your temps. if you can't turn up the thermostat, or don't have an oil space heater, don't bother. its not that cold and it growth might be slowed by a day or two in the end.  so nothing to fuss about.

but I do have some concerns... nothing concrete, just concerns.

like the first pic. that is a lot of growth for 5 days after a spore inoculation. I'm usually lucky if my spores have germinated by then.

you might indeed be over-concerned. don't be handling the jars a bunch. this will cause your filter layer to settle down into the jar, possibly spreading contaminates down into uncolonized substrate. so just set the jars in their resting place, and wait it out.

also, start another project if you can. grab some more jars and do another batch. having multiple projects helps cut down on the down time and allows you to practice your techniques.

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Offlinedrege
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Registered: 11/04/14
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21275510 - 02/15/15 12:33 AM (9 years, 3 months ago)

Well I know I don't have any grows under my belt as of yet but the last thing I remember was to just put em on a shelf and leave em alone,every time you muck about with the jars you create settling witch packs the sub down and will cause diminished colonization and or flat out stall - don't handle them so much.


--------------------

https://discord.gg/hqdy5ymn

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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,346
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Re: Incubation temp - below 70˚f? [Re: drege]
    #21275512 - 02/15/15 12:34 AM (9 years, 3 months ago)

:whathesaid:

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Invisible3iRiS9
@psilocybeing
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Re: Incubation temp - below 70˚f? [Re: Munchauzen]
    #21275619 - 02/15/15 01:33 AM (9 years, 3 months ago)

:facepalm: Now that I think of it, I've probably done more harm than the temp by picking it up to take pictures and notes.
Hahaha, I'll just tell myself every mistake is merely an opportunity to improve technique.

Glad to hear some feedback!
I'm going to leave em alone from now on.

Oh, as for the concerns about fast colonization, I've scoped out the mycelium with a loup and it all seems to resemble P. cubensis.
Would Cobweb be more erratic and growing in a net, rather than flowing in a certain direction?
In every jar, the edges flow significantly, but the inoculation points seem to have a whole mess of growth going on.
I figured that the flowing edges of the myc. pretty much gives it away as P. cubensis.

Are there any macroscopic differences in P. cubensis mycelium and common contaminants that would give some reassurance?

Thanks a ton!

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OfflineMushroom_J
Hard to the Coir !
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Registered: 02/17/11
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21275651 - 02/15/15 01:53 AM (9 years, 3 months ago)

You at least have good pictures though!
Can actually see shit instead of reflections lol.
the 20x looks nice.


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Offlinedrege
This space for lease

Registered: 11/04/14
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Re: Incubation temp - below 70˚f? [Re: Mushroom_J]
    #21275693 - 02/15/15 02:35 AM (9 years, 3 months ago)

I plan on making lots of mistakes, but none of them will be any that others on the threads I have read have made.

(not a snide remark, just a matter of fact useless post)

Being wrong or ignorant is not a bad thing, it should be rejoiced, and being shown error should be a happy learning experience - all too much ego lowers people to anger when doubt arises as to knowledge.

I'm screwed though, indoor temps here have been upwards of 75° and its not feasible/financially conscionable to air condition my grow room.


--------------------

https://discord.gg/hqdy5ymn

Edited by drege (02/15/15 02:36 AM)

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Invisible3iRiS9
@psilocybeing
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Re: Incubation temp - below 70˚f? [Re: drege]
    #21275841 - 02/15/15 05:10 AM (9 years, 3 months ago)

The more I look at this jar, the less confident I feel.
It just doesn't seem very ropey, and it is growing pretty fast, contrary to what I believed at the beginning of this thread.
Does this seem typical for multi-spore genetics?





It looks like it's flowing in one direction, but it doesn't appear as thick and rhizomorphic when compared to these photos:



:shrug:
Again, maybe it's that first grow anxiety kicking in. Haha
Maybe I'm looking for thick, ropey, rhizomorphic mycelium when in fact, I just have the effects of multi-spore genetics causing a less typical formation!?
I hope so!

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Invisiblefunkyfish77
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Registered: 06/10/10
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21275868 - 02/15/15 05:42 AM (9 years, 3 months ago)

They don't always get ropey.  They look fine . Be patient that's one thing anyone in this hobby needs.
Good luck

Funky

Edited by funkyfish77 (02/15/15 05:43 AM)

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Offlinegermanshroomer
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Registered: 02/15/15
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Re: Incubation temp - below 70˚f? [Re: funkyfish77]
    #21277336 - 02/15/15 12:32 PM (9 years, 3 months ago)

i also think they look fine

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OfflinetaGyo
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Re: Incubation temp - below 70˚f? [Re: germanshroomer]
    #21277384 - 02/15/15 12:42 PM (9 years, 3 months ago)

Those are fine.

That's called Tomentose growth, the ropey is called Rizomorphic.

Rhizo:


Tomentose:



--------------------
Gyo's Better Grows
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Dominus fortunae meae sum

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Invisible3iRiS9
@psilocybeing
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Re: Incubation temp - below 70˚f? [Re: taGyo]
    #21279180 - 02/15/15 06:32 PM (9 years, 3 months ago)

Quote:

taGyo said:
Those are fine.

That's called Tomentose growth, the ropey is called Rizomorphic.





:thumbup: Sweet, Helpful info.
I wouldn't have known without this post.
I thought all P. cubensis mycelium would be rhizomorphic in appearance.
Thank you!

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OfflinetaGyo
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21279183 - 02/15/15 06:32 PM (9 years, 3 months ago)

:pipesmoke:


--------------------
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TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlinespacechildo
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Re: Incubation temp - below 70˚f? [Re: taGyo]
    #21279226 - 02/15/15 06:39 PM (9 years, 3 months ago)

Your pics look better than "these photos" that you posted which I cant zoom in on but look bacterial to me.

nothing is to be expected from MS, its a wild card every time.

Just leave them alone and I'm pretty confident they will turn out fine.
your 20x zoom pics looks awesome, perfect mycelium (as in no bacteria)

Make a new batch if its hard not to touch these jars :tongue2:

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OfflinetaGyo
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Re: Incubation temp - below 70˚f? [Re: spacechildo]
    #21279232 - 02/15/15 06:40 PM (9 years, 3 months ago)

Yeah his pics are actually really on point, very detailed.

What did you use?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Invisible3iRiS9
@psilocybeing
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Re: Incubation temp - below 70˚f? [Re: taGyo]
    #21279300 - 02/15/15 06:53 PM (9 years, 3 months ago)

Quote:

taGyo said:
Yeah his pics are actually really on point, very detailed.

What did you use?




Hahaha, Thank you!!

It's nothing fancy.
I'm an amateur jeweler as well, so I've gotten used to taking phone pictures with a jeweler's loupe sticky-tacked to the camera. Works great for up close shots of gems and minerals too.

I've used a Dslr through a loupe, but it gives you a pretty small area of focus.
If you don't have a decent macro lens, it's better than nothing!

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OfflinetaGyo
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Re: Incubation temp - below 70˚f? [Re: 3iRiS9]
    #21279305 - 02/15/15 06:53 PM (9 years, 3 months ago)

Niice,

Gotta buy myself a macro lens.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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