Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   MagicBag.co Certified Organic All-In-One Grow Bags   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21273251 - 02/14/15 01:12 PM (9 years, 10 months ago)

Which is why that's an unfair comparison.  G2G should be done in sterile air.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: AGAR'S GRAIN LC [Re: Psilicon]
    #21273258 - 02/14/15 01:14 PM (9 years, 10 months ago)

Quote:

van der griegen said:
Which is why that's an unfair comparison.  G2G should be done in sterile air.




still makes GLC safer imo. Dont you see all these people with SABS getting bacteria and mold in their tubs.

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21273270 - 02/14/15 01:17 PM (9 years, 10 months ago)

No, not that many.  Usually it's still air bathrooms.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
Trusted Cultivator
Re: AGAR'S GRAIN LC [Re: Psilicon]
    #21273276 - 02/14/15 01:18 PM (9 years, 10 months ago)

Do G2G in a SAB and watch your success rate improve. Its not the fault of the method if you choose to implement it incorrectly. Again I am not bashing GLC but there is a reason commercial operations do not expand with that method. Most use G2G or LI to expand. FrankHorrigan experienced his biggest contam meltdown with GLC. I'm sure most people who are familiar with him agree he knows his stuff. If he can lose 24 tubs with it anyone can. Understand the pitfalls. GLC will work great until it doesn't. The biggest risk with liquids especially GLC is that when it does let you down,  it lets you down big.

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: AGAR'S GRAIN LC [Re: Pastywhyte]
    #21273322 - 02/14/15 01:32 PM (9 years, 10 months ago)

Considering that 1 master jar of GLC can produce 100x as much as 1 G2G master jar and I can test out a GLC and know if its good or not within a week is the reason it has my love. Not to mention that I dont have to work in a little plastic box. Testing out a GLC before MASSIVE expansion is like following up with a MASSIVE institution ~ Gotta do it or deal with the risk of getten fucked.


Is this not what agar is for? Talk about it enough. lol

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
Trusted Cultivator
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21273339 - 02/14/15 01:38 PM (9 years, 10 months ago)

That is what agar is for. If your inoculating your master with agar and testing it with agar then you have little to worry about. But if I was using agar I would just inoculate LC and remove the extra stick of the SHIP or the risk of germinated endospores. Takes less time to colonize an LC with a nice agar wedge than it does to colonize a grain master, if speed is your concern.

Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21273341 - 02/14/15 01:39 PM (9 years, 10 months ago)

Quote:

blackdust said:
I can test out a GLC and know if its good or not within a week is the reason it has my love.




Are you saying that you can take a glc, inoculate more jars, spawn those jars, and get fruits within a week?

Or are you saying you test it out on agar for contams first?

Considering that PCing doesn't kill everything on grains, I hope you test that GLC regularly if you store it.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

Edited by SpitballJedi (02/14/15 01:41 PM)

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: AGAR'S GRAIN LC [Re: SpitballJedi]
    #21273405 - 02/14/15 02:02 PM (9 years, 10 months ago)

Quote:

SpitballJedi said:


Or are you saying you test it out on agar for contams first?

Considering that PCing doesn't kill everything on grains, I hope you test that GLC regularly if you store it.




Yes. I actually tested my fist GLC on cakes as shown in the OP. I would recommend testing on agar before using to not repeat what happened to Frank. Or test on a small batch of cakes and then slurry the cakes to grain jars. 1 cake can make 50 grain jars. I want to try this GLC again. Now that I know Eat can fully colonize a PF cake in 4 days I want to try the same with a GLC instead of steam tissue.

Extras: Filter Print Post Top
OfflineYerow
Stranger
Male User Gallery


Registered: 09/22/14
Posts: 1,206
Last seen: 3 years, 7 months
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21273492 - 02/14/15 02:25 PM (9 years, 10 months ago)

Think it would help/do anything to let that water sit in the jar for a few hours, maybe shake a few times in that time, to really get that myc out into it?

Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
OG Cultivator
Re: AGAR'S GRAIN LC [Re: SpitballJedi]
    #21273625 - 02/14/15 03:05 PM (9 years, 10 months ago)

back from the dead. good to see you bd.

hey sbj been a long time man how ya been?

oh guess what i just made sbj?

i made all my tub hole magnets! colonizing 4 tubs now with the magnets on and then ill put the Dilated filters on next during fruiting. i did 4 tubs with 8 quarts of spawn to 11 quarts of substrate. sub depth was about 4". going to case in about 3 days when the subs finish colonizing. i haven't been able to beat my 3 day bulk colonization average yet. but im trying hard. i don't think it can get any faster. i did make some slight modifications. i would usually spawn my tubs with the polyfil in the holes and put the tub in a draft free area. now ive started using the magnets to colonize the tubs in my grow room with the fan moving air. then switch up to poly and dilated filters when fruiting. the issues i was having for the last several months i have worked out. which was directly related to how tight i was stuffing my bottom polyfil holes. i had them in, but not solid bulging tight. i made that minor change, built a new grow setup with a small fan and its working rock solid now. i also started using a 6500 light rather than natural daylight. the ceiling fan wasn't cooperating lol. the lighting isn't making much of a difference other than the fruits grow directly toward it rather than all over the tub with diffused light. yields still the same in cased and uncased bulks, natural diffused light or artificial light. doing about 6-9z dry average per tub.  ill pm ya the magnet pics.

Quote:

SpitballJedi said:

Are you saying that you can take a glc, inoculate more jars, spawn those jars, and get fruits within a week?




GLC takes me about 7-9 days for full colonization in quart grain jars.
for grain slurry to bulk=2 weeks from inoculation to harvest. 12-13 days on average for slurry. but a GLC takes me about 5 extra days to colonize quarts. so for GLC 18 days till fruit from inoculation of grain jars to harvest when your spawning to bulk tubs. if you fruit directly from bottles that would decrease the time by a few days up to a week.

i don't use a SAB for g2g or any of that and i get near 100% success without contamination. i don't think the method matters. if its a flowhoood, shmuv, sab, its your technique and following the principle using that method that ensures success. ive used all 3 and the contam rate is the same.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: AGAR'S GRAIN LC [Re: eatyualive]
    #21273997 - 02/14/15 04:59 PM (9 years, 10 months ago)

Hey eat, good to see you. It has been a while. Glad to see you're still kicking ass. :lol:

The time scale you mention seems reasonable.

Like PW mentioned, why make GLC instead of Agar LC? We've talked enough to know this is an honest question. You've inspired me to question my methods in the past and I've actually changed some things to make it easier because of it.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
OG Cultivator
Re: AGAR'S GRAIN LC [Re: SpitballJedi]
    #21274936 - 02/14/15 09:21 PM (9 years, 10 months ago)

Quote:

SpitballJedi said:
Hey eat, good to see you. It has been a while. Glad to see you're still kicking ass. :lol:

The time scale you mention seems reasonable.

Like PW mentioned, why make GLC instead of Agar LC? We've talked enough to know this is an honest question. You've inspired me to question my methods in the past and I've actually changed some things to make it easier because of it.



your not really making a GLC. its almost already there before you. you simply blend up the grain in the water and you have a very large volume of LI for inoculation. i guess you could say for convenience. ill give an example, i g2ged 10 quart jars. thats all i have going(maybe you have a situation where your growth stalled or life got in the way). i want to spawn 2 tubs and need a quick turnaround to get more spawn going for another round of tub spawning and flushes. i could 1)g2g one last quart and make 10 more quarts for spawn. or i could keep that 1 grain jar and make enough inoculant for over 100 quart jars. its easy because you already have a large volume of culture that you can transfer. you could use a plate. but it all really depends on the situation. if your using an LI to inoculate a bunch of grain jars, use that last grain jar for a GLC. and then you spawn the other 9 jars. then that one GLC turned into enough spawn for 20 tubs or more.

1 its easy. and if you dont' have any agar plates or anything of that nature you can use your last remaining grain jar as a GLC to transfer and make a ton more jars.

2. that one grain jar can inoculate enough jars comparable to 10 or more plates of LI. you could also use a grain from that remaining quart and put it on agar. but then you would need to grow that out on a plate. when you already have a large volume of inoculum at your disposal. the way i look at it is, if you hav a quart volume of liquid inoculant. you have an unlimited amount of spawn that you can make in a moments notice, and its convenient and not that difficult.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (02/14/15 09:28 PM)

Extras: Filter Print Post Top
InvisibleNeomorph
Male


Registered: 12/10/14
Posts: 767
Loc: Europe
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21278349 - 02/15/15 03:17 PM (9 years, 10 months ago)

Nice work dude, the jar has only water or it has honey to ?

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: AGAR'S GRAIN LC [Re: Neomorph]
    #21278352 - 02/15/15 03:18 PM (9 years, 10 months ago)

Quote:

kastriotr said:
Nice work dude, the jar has only water or it has honey to ?




distilled water that has been pressure cooked along with the syringe.

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 16 days
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21313151 - 02/22/15 01:40 PM (9 years, 10 months ago)

After completing this do you toss the grain in the jar or will it recover? I am about to try this out.

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: AGAR'S GRAIN LC [Re: blazedup]
    #21313248 - 02/22/15 01:48 PM (9 years, 10 months ago)

let the jar recover.

Extras: Filter Print Post Top
Offlineblazedup
Dirk Diggler
Male User Gallery

Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 16 days
Re: AGAR'S GRAIN LC [Re: blackdust]
    #21313275 - 02/22/15 01:54 PM (9 years, 10 months ago)

Quote:

blackdust said:
let the jar recover.




Thanks :thumbup:

Extras: Filter Print Post Top
OfflineTrIpPyDuDe
FUCK
Male User Gallery

Registered: 12/07/14
Posts: 1,263
Loc: Kentucky Flag
Last seen: 9 years, 9 months
Re: AGAR'S GRAIN LC [Re: blazedup]
    #21313311 - 02/22/15 02:03 PM (9 years, 10 months ago)

I just bought pre poured agar plates. Can't wait to use them


--------------------
http://www.shroomery.org/forums/showflat.php/Number/21330463

I'm looking for prints PM me

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   MagicBag.co Certified Organic All-In-One Grow Bags   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Agar help please Fr0ggy 3,108 17 05/10/03 06:19 PM
by Magash
* Agar and growing that white stuff! pj541 2,666 5 10/15/01 04:06 PM
by pj541
* The Agar Artwork Collection
( 1 2 3 4 all )
natedawgnow 3,004 62 12/21/20 02:07 PM
by verytastycheese
* Josex' Poke: A different way of doing LC.
( 1 2 3 4 ... 80 81 )
Josex 135,108 1,617 06/30/24 02:25 PM
by Buckdog
* Agar to Grain - slow growth?
( 1 2 all )
Ridcully 3,286 20 09/01/18 03:48 PM
by jazzcabbage420
* Agar question sill_o_cin 4,095 4 11/21/01 05:23 PM
by sill_o_cin
* Test Tube + Agar Q x_bonerz_x 839 1 02/24/03 05:52 PM
by KingCobWeb
* Benefits of Agar? celsius 9,660 10 12/01/19 12:36 AM
by wildernessjunkie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
12,827 topic views. 25 members, 118 guests and 73 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.01 seconds on 13 queries.