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InvisibleOmnibeaster
Stranger


Registered: 02/03/15
Posts: 121
consistent lid design results! * 1
    #21263615 - 02/12/15 12:28 PM (9 years, 3 months ago)

Pictured below are 6 milo/millet jars spawned with b+ slurry on the same night and incubated in the same ice cooler. Each collumn contains 2 jars with the same lid design for comparison. The lids of the first 2 jars were made by securing a small dime sized ball of polyfill over single ~8mm holes with 1 strip of micropore tape. The second column jars were outfitted with 29mm synthetic filter disks mounted with RTV over single ~8mm holes. Lastly, the third column jars were incubated sporting their original metal lids+rings tightened snugly with no modifications. As you can see, the first 2 jars display brighter, more dense, and consolidated mycelium than the second column jars while the 3rd column jars are considerably less fully colonized than either of the first two columns. I used five jars a piece for each lid design and the results were perfectly consistent across the board. Clearly lids modified with small puffs of polyfill plugged over ~8mm holes and fixed with micropore tape provides the best gas exchange while still providing efficient protection against contams in this experiment. Synthetic filter disks were a close second so considering they are reusable I still feel they are the best most clean-cut option but I thought the results were very intreaguing and worth a look regardless. Also, as a side note, I have found that milo seems to colonize much better than millet. I'll provide a pic of the polyfill/micropore design when I get home.



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Do you feel like a kitty cat yet?

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OfflineMBabble
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Re: consistent lid design results! [Re: Omnibeaster]
    #21263638 - 02/12/15 12:37 PM (9 years, 3 months ago)

Interesting, thanks for posting!

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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
Loc: In your head
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Re: consistent lid design results! [Re: MBabble]
    #21263660 - 02/12/15 12:41 PM (9 years, 3 months ago)

6 jars is a really small sample size. Probably need a multiple of 10 or even 100 to be able to say anything conclusive. Keep sampling and see if results are consistent


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

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InvisibleOmnibeaster
Stranger


Registered: 02/03/15
Posts: 121
Re: consistent lid design results! [Re: Bugler Boy]
    #21263701 - 02/12/15 12:51 PM (9 years, 3 months ago)

Quote:

Bugler Boy said:
6 jars is a really small sample size. Probably need a multiple of 10 or even 100 to be able to say anything conclusive. Keep sampling and see if results are consistent




I realize that but for my own purposes it is conclusive enough. Keep in mind I did use 5 jars each, 15 jars total. Someone with more hands an a bigger PC than mine would have to do it for definitive results. I just observed the results and I had to share


--------------------
Do you feel like a kitty cat yet?

Edited by Omnibeaster (02/12/15 01:01 PM)

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OfflineMastaBlastar
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Registered: 04/06/13
Posts: 1,069
Loc: Barter Town, AUS
Last seen: 6 years, 10 months
Re: consistent lid design results! [Re: Omnibeaster]
    #21263924 - 02/12/15 01:40 PM (9 years, 3 months ago)

That is not at all conclusive IMO.  Do at least 100.  And RYE, RYE RYE RYE all day long.  Cant beat it.  Either grain for psilos or seed for others its IME the best/easiest/most tolerant spawn medium.  There is a reason commercial growers use rgs.


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Everything I have said, may say, will say, am thinking about saying and/or thinking/typing/dreaming/writing is in all likelyhood made up and has no factual basis in reality whatsoever, and is likely all plagiarized and copy pasted straight from someone else, so get mad at them .  Just a warning

Edited by MastaBlastar (02/12/15 01:43 PM)

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InvisibleOmnibeaster
Stranger


Registered: 02/03/15
Posts: 121
Re: consistent lid design results! [Re: MastaBlastar]
    #21264159 - 02/12/15 02:39 PM (9 years, 3 months ago)

Right. Its just consistent and could warrant further investigation.


--------------------
Do you feel like a kitty cat yet?

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OfflineKizzle
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Registered: 08/30/11
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Re: consistent lid design results! [Re: Omnibeaster]
    #21264685 - 02/12/15 04:19 PM (9 years, 3 months ago)

Why not just stick the polyfill through the hole? It holds itself in, better than the tape would even.


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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: consistent lid design results! [Re: Kizzle]
    #21264719 - 02/12/15 04:25 PM (9 years, 3 months ago)

Great demonstration of the importance of GE.

:kaneclap:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineFreeWorldOrder
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Registered: 12/24/13
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Re: consistent lid design results! [Re: Kizzle]
    #21264726 - 02/12/15 04:26 PM (9 years, 3 months ago)

I don't see why anyone would want to bust OP's balls.

He did an experiment, observed what happened, and posted his results.

No where in his post did I see him say the results were 100% definitive, just intriguing.

Great post man.

This deserves :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


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"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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Agar's Liquid Inoculant TEK

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OfflineKizzle
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Re: consistent lid design results! [Re: FreeWorldOrder]
    #21264755 - 02/12/15 04:31 PM (9 years, 3 months ago)

Not busting balls. Just asking a question. I used to feel uncomfortable with the idea of having it stick down into the substrate so I suspect that's why. It's really not a big deal though. Was just curious if that's why.


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OfflineFreeWorldOrder
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Re: consistent lid design results! [Re: Kizzle]
    #21264825 - 02/12/15 04:47 PM (9 years, 3 months ago)

Was talking about the posts saying there weren't enough jars to tell...not yours Kizzle...lol.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK

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OfflineBugler Boy
Cultivar


Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 3 years, 2 days
Re: consistent lid design results! [Re: FreeWorldOrder]
    #21264846 - 02/12/15 04:52 PM (9 years, 3 months ago)

Quote:

FreeWorldOrder said:
Was talking about the posts saying there weren't enough jars to tell...not yours Kizzle...lol.




Theres not


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: consistent lid design results! [Re: Bugler Boy]
    #21264873 - 02/12/15 04:57 PM (9 years, 3 months ago)

Quote:

Bugler Boy said:
Quote:

FreeWorldOrder said:
Was talking about the posts saying there weren't enough jars to tell...not yours Kizzle...lol.




Theres not




Depends on when the other four finished.  I can do the math if you give me the numbers, as exact as you can, OP.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineBugler Boy
Cultivar


Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 3 years, 2 days
Re: consistent lid design results! [Re: Psilicon]
    #21264925 - 02/12/15 05:04 PM (9 years, 3 months ago)

Quote:

van der griegen said:
Quote:

Bugler Boy said:
Quote:

FreeWorldOrder said:
Was talking about the posts saying there weren't enough jars to tell...not yours Kizzle...lol.




Theres not




Depends on when the other four finished.  I can do the math if you give me the numbers, as exact as you can, OP.




Lets think this through a little more.. how about genetics? I'm guessing it was MS. Theres a crapshoot for speed of colonization right there and a HUGE factor no one has considered yet

To really test this you'd want a proven isolate and LOTS of jars (100s of each type of filter)


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: consistent lid design results! [Re: Bugler Boy]
    #21264949 - 02/12/15 05:09 PM (9 years, 3 months ago)

Quote:

Bugler Boy said:

Lets think this through a little more.. how about genetics? I'm guessing it was MS. Theres a crapshoot for speed of colonization right there and a HUGE factor no one has considered yet

To really test this you'd want a proven isolate and LOTS of jars (100s of each type of filter)




We can say things about humans or horses or orange trees pretty definitively without an isolate.  :grin:  We're all pretty MS.  How do we do that?


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: consistent lid design results! [Re: Psilicon]
    #21264961 - 02/12/15 05:11 PM (9 years, 3 months ago)

if only humans could be started on agar:eek:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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OfflineBugler Boy
Cultivar


Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 3 years, 2 days
Re: consistent lid design results! [Re: Psilicon]
    #21265004 - 02/12/15 05:15 PM (9 years, 3 months ago)

Quote:

van der griegen said:
Quote:

Bugler Boy said:

Lets think this through a little more.. how about genetics? I'm guessing it was MS. Theres a crapshoot for speed of colonization right there and a HUGE factor no one has considered yet

To really test this you'd want a proven isolate and LOTS of jars (100s of each type of filter)




We can say things about humans or horses or orange trees pretty definitively without an isolate.  :grin:  We're all pretty MS.  How do we do that?




Because we're not fungi. Do any of the above spp you mentioned have a dikaryotic stage?


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: consistent lid design results! [Re: Bugler Boy]
    #21265044 - 02/12/15 05:19 PM (9 years, 3 months ago)

Quote:

Bugler Boy said:
Quote:

van der griegen said:
Quote:

Bugler Boy said:

Lets think this through a little more.. how about genetics? I'm guessing it was MS. Theres a crapshoot for speed of colonization right there and a HUGE factor no one has considered yet

To really test this you'd want a proven isolate and LOTS of jars (100s of each type of filter)




We can say things about humans or horses or orange trees pretty definitively without an isolate.  :grin:  We're all pretty MS.  How do we do that?




Because we're not fungi. Do any of the above spp you mentioned have a dikaryotic stage?




Nope. But all of them have a diploid stage, which has the same amount of genetics.  I don't see how the number of nuclei is really important.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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InvisibleOmnibeaster
Stranger


Registered: 02/03/15
Posts: 121
Re: consistent lid design results! [Re: Kizzle]
    #21266734 - 02/12/15 10:26 PM (9 years, 3 months ago)

Quote:

Kizzle said:
Why not just stick the polyfill through the hole? It holds itself in, better than the tape would even.




I just tried for the very reason that I don't like the idea of the poly fill coming in contact with the grains while shaking but mostly I just liked the clean cut design and the micropore makes me feel a little more secure. Even if it realy doesnt, I like to think it adds a little insurance. Also, it actually holds very snug and could probably be used multiple times although I wouldn't. The design uses a minimal amount of material anyway. I forgot to mention I have since tested jar lids stuffed with polyfill only. They performed pretty much the exact same as the synthetic filter disk lids. The experiment indicates that obviously the less dense/thick filter material is, the quicker the colonization.



--------------------
Do you feel like a kitty cat yet?

Edited by Omnibeaster (02/12/15 10:30 PM)

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