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OfflinePsilocyBen17
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Re: First Agar Inoculations....when to shake? [Re: budmanman]
    #21262605 - 02/12/15 07:31 AM (9 years, 3 months ago)

Next time I do agar I'm gunnah throw 2 or 3 wedges in per jar. I had a lot of left over agar :frown: I dont know why I never thought of this...


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OfflinetaGyo
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Re: First Agar Inoculations....when to shake? [Re: spacechildo]
    #21262677 - 02/12/15 07:56 AM (9 years, 3 months ago)

If you understand shaking you never have to ask to shake.

When you do A2G, shake the SHIT out of that, don't even worry about the chunks of Mycelium that are coming off the agar because they'll recover. In fact the MORE myc you knock off the agar the BETTER for faster colonization.

You shake to distribute colonized grains all around the jar. If you don't have enough colonized grains to be equally distributed then you're wasting your mycleium's vigor.

Quote:

spacechildo said:
stealing 13's pic but its just so fitting!



aka dont shake yet :wink:



:lmafo:


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OfflinePsilocyBen17
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Re: First Agar Inoculations....when to shake? [Re: taGyo]
    #21262699 - 02/12/15 08:01 AM (9 years, 3 months ago)

So your saying I should shake immediately after I drop my wedges in the jar?


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Invisiblewowimflabbergasted
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Re: First Agar Inoculations....when to shake? [Re: taGyo]
    #21262720 - 02/12/15 08:09 AM (9 years, 3 months ago)

gyo lol


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Invisiblewowimflabbergasted
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Re: First Agar Inoculations....when to shake? [Re: PsilocyBen17]
    #21262721 - 02/12/15 08:09 AM (9 years, 3 months ago)

Quote:

PsilocyBen17 said:
So your saying I should shake immediately after I drop my wedges in the jar?



Yeah dude...that's what I said earlier.


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Offlineurthtown
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Re: First Agar Inoculations....when to shake? [Re: wowimflabbergasted]
    #21262755 - 02/12/15 08:21 AM (9 years, 3 months ago)

Quote:

wowimflabbergasted said:
Quote:

PsilocyBen17 said:
So your saying I should shake immediately after I drop my wedges in the jar?



Yeah dude...that's what I said earlier.




That's what I do. Nocc with agar, shake a bunch. At 20-30% shake again and then leave it until it's ready to spawn. I usually notice all kinds of myc popping up about 5 days after noccingwith agar wedges that is growing wherever the agar rolled around and touched something. If you do this you'll get to 20-30% much faster, which means you can shake sooner which means it'll recover and fully colonize sooner.


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Re: First Agar Inoculations....when to shake? [Re: urthtown]
    #21262768 - 02/12/15 08:25 AM (9 years, 3 months ago)

Myc spreads in all directions,

Utilize it.


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Invisiblestareatclouds
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Re: First Agar Inoculations....when to shake? [Re: taGyo]
    #21262782 - 02/12/15 08:29 AM (9 years, 3 months ago)

I'm pretty sure you're not supposed to shake ASAP as it delays the colonization since the myc is recovering. I dug up this RR quote about it.

Quote:

RogerRabbit said:
Those look fine, but you would have been better off not to shake as directed above.

The reason is that when you cut off a wedge, you cut through the growing, leading edge of the mycelium.  It's best to let this recover without further damage, so that the mycelium crawls off the wedge and into the grains below.  It makes little difference if the wedge is upside down or right side up because it's not going to recover and grow from the middle, but from the leading edges.

Once the mycelium has crawled into a few of the grains below, shake to distribute.  Shaking early with a mycelium wedge slows things down because you only get recovery later from the wedge itself, not from all the mycelium which falls off when you shake, but doesn't recover and grow.  You must then wait for what's left on the agar wedge to recover and grow again before the mycelium can leap off, setting things back a few days.

The other advantage to not shaking right away is that you can see the agar wedge on top so that if green mold or another contaminant develops, you see it before you shake so you can discard that jar.
RR




Quote:

RogerRabbit said:
Shaking the grain jar after inoculation with agar knocks all the mycelium off the wedge, but it doesn't recover into the grains.  Instead, it has to grow back on the agar wedge, and then it can crawl into the grains.  Shaking slows down the process over simply dropping the wedge on top and letting the mycelium crawl off when it's ready.  Shake when a few dozen grains on top are covered.



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Re: First Agar Inoculations....when to shake? [Re: stareatclouds]
    #21262790 - 02/12/15 08:31 AM (9 years, 3 months ago)

I tiger drop my wedges so I don't cut through anything,

Also that would take forever. I've done A2G where I just dropped the wedge on top and it literally just sits there until it fuzzes up enough to leap off. Also did it where I shook the shit out of it. Yeah it took a little to recover but I had myc everywhere in my jar.


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Invisiblestareatclouds
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Re: First Agar Inoculations....when to shake? [Re: taGyo]
    #21262793 - 02/12/15 08:33 AM (9 years, 3 months ago)

I'm sure YRMV. Just spreading along more RR quotes about it. His older posts suggest instantly shaking. I rarely do A2G because it takes forever, but when I do, I cut the wedge into 1/4s and drop in diff areas.

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Offlinespacechildo
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Re: First Agar Inoculations....when to shake? [Re: urthtown]
    #21262824 - 02/12/15 08:43 AM (9 years, 3 months ago)

Quote:

urthtown said:
I usually notice all kinds of myc popping up about 5 days after noccingwith agar wedges that is growing wherever the agar rolled around and touched something.




this is my experience as well!

what do you do ifyou dont do agar2grain stare?

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Invisiblestareatclouds
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Re: First Agar Inoculations....when to shake? [Re: spacechildo]
    #21262862 - 02/12/15 08:55 AM (9 years, 3 months ago)

I use LIs and slurries to inoculate my grains, mostly. I experimented with a big jar of agar LC that I recently used on tons of shit (it was clean), but I doubt I'll do that again.

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Re: First Agar Inoculations....when to shake? [Re: stareatclouds]
    #21263123 - 02/12/15 10:05 AM (9 years, 3 months ago)

Yeah I do little half pint jars of LC with these lids. You can do a lot of grain with just a half pint and I've had great success with almost no contams in open air (injection ports) or SAB.



Draw up and purge syringes with water boiled for 10 min half a dozen times. Let them cool the last time and inject the water into the LC to keep constant volume as you draw off myc. :thumbup: So much faster than A2G. That said I still do plenty of G2G and A2G when I have side projects!

Case and point though - I have 5 galindoi quarts that were done A2G January 10th and 5 that were done with MSS injection January 5th and 5 that were done with MS LC on January 22 and they all just hit 100% at the same time. Lulz :rofl: at least none of them stalled after the shake!


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Edited by urthtown (02/12/15 11:09 AM)

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OfflinePsilocyBen17
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Re: First Agar Inoculations....when to shake? [Re: urthtown]
    #21263222 - 02/12/15 10:30 AM (9 years, 3 months ago)

Wow, great thread guys. Thx for digging up so much RR knowledge.


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Invisiblewowimflabbergasted
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Re: First Agar Inoculations....when to shake? [Re: stareatclouds]
    #21263390 - 02/12/15 11:13 AM (9 years, 3 months ago)

Quote:

stareatclouds said:
I'm pretty sure you're not supposed to shake ASAP as it delays the colonization since the myc is recovering. I dug up this RR quote about it.
Quote:

RogerRabbit said:
Shaking the grain jar after inoculation with agar knocks all the mycelium off the wedge, but it doesn't recover into the grains.  Instead, it has to grow back on the agar wedge, and then it can crawl into the grains.  Shaking slows down the process over simply dropping the wedge on top and letting the mycelium crawl off when it's ready.  Shake when a few dozen grains on top are covered.






I don't buy it



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Invisibled0urd3n
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Re: First Agar Inoculations....when to shake? [Re: wowimflabbergasted]
    #21263416 - 02/12/15 11:19 AM (9 years, 3 months ago)

Yeah I think it probably depends on the wedge size. If you use a pretty small wedge it might not be the best idea. If you use half a plate shaking would be better it seems to me.


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Re: First Agar Inoculations....when to shake? [Re: d0urd3n]
    #21263424 - 02/12/15 11:21 AM (9 years, 3 months ago)

All depends I guess.


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OfflinePsilocyBen17
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Re: First Agar Inoculations....when to shake? [Re: wowimflabbergasted]
    #21263433 - 02/12/15 11:24 AM (9 years, 3 months ago)

I decided to settle the argument for you boys. I shook the jar that was colonized the most. I'll post a pic of how it looks in a few days time.


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Re: First Agar Inoculations....when to shake? [Re: PsilocyBen17]
    #21263451 - 02/12/15 11:31 AM (9 years, 3 months ago)

:kenthumbup:


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Re: First Agar Inoculations....when to shake? [Re: PsilocyBen17]
    #21263463 - 02/12/15 11:36 AM (9 years, 3 months ago)

I definitely agree with the others who say wait until 20-30%... If you shake now they will probably either stall or take forever to recover what  little mycelium there is. You don't want to rush anything when growing mushrooms. You will be greatly rewarded for being patient when it comes to this hobby...:thumbup:


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