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Offlineesore
Stranger
Registered: 10/08/14
Posts: 22
Last seen: 8 years, 9 months
My first grow attempt failed to colonize.
    #21260061 - 02/11/15 06:19 PM (9 years, 3 months ago)

I made a thread a few weeks ago about my five PF tek jars not colonizing. Three were showing nothing and 2 were showing slight growth. Well now 30 days after inoculation the 3 jars are still empty.

Of the two jars that had some growth, jar B looks like cobweb mold and jar A only has growth on one side (though the growth does look nice) and a spot of what looks like white mold on the other side where I injected.

Jar A good side (front view & edge of growth):


Jar A bad side:


Jar B:

(sorry it looks blurry, my jars have slightly patterned glass on the outside)

The growth in jar B has stalled and looks fluffy compared to the stringy texture in jar A, leading me to believe it is mold. The other side of jar B has nothing and the other 3 jars have nothing at all as well.




Last wednesday I decided to give up and make another 5 jars. I'm doing a few things differently.

Summary of things I'm doing different:
-Jar lids instead of foil lids
-More water (used the 2-1-1 ratio)
-4 injection holes instead of 2
-Did not cover the holes after inoculation

Now for my current concern. When I had finished the 60 minute pressure cooking I took the jars out and let them cool for 12 hours. When I took the foil off there was water droplets on the lid. Does that mean my dry verm layer got wet?
I don't want to waste another 30 days on botched jars, help is much appreciated!


edit- removed unnecessary details

Edited by esore (02/11/15 06:42 PM)

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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
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Re: My first grow attempt failed to colonize. [Re: esore]
    #21260119 - 02/11/15 06:29 PM (9 years, 3 months ago)

Those pictures are great.

In this hobby condense things to a few lines, I skimmed sorry.

Throw the jars out that haven't shown growth, who cares what contam it was. Next time remove the foil after pressure cooking. It looks like you failed because you restricted Gas Exchange with the rubber band. Cobweb normally forms because of High CO2 and stale air.

All those jars look fucked, Jar B I would wait for another opinion.

mushroomvideos.com/BRF-Pf-Tek

Have you seen those videos?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflineBugler Boy
Cultivar


Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 3 years, 3 days
Re: My first grow attempt failed to colonize. [Re: taGyo]
    #21260145 - 02/11/15 06:34 PM (9 years, 3 months ago)

Take the foil off after the PC. The foil prevents the dry verm from getting wet.

If you leave it on steam will condense under the foil and on the lid as the jars cool making your dry verm barrier useless.

The verm barrier is your filter, not the foil.


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

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Offlineesore
Stranger
Registered: 10/08/14
Posts: 22
Last seen: 8 years, 9 months
Re: My first grow attempt failed to colonize. [Re: taGyo]
    #21260163 - 02/11/15 06:38 PM (9 years, 3 months ago)

The foil was instead of lids, it had holes. I took the pressure cooking foil (without holes) off after. Here is my recent batch vs old batch:





You guys aren't telling me to remove the lids and leave exposed verm, right? RR leaves the lids on in his videos. My tin foil layer was just instead of the lids. (that's what it suggested as an option in the guide I followed for my first grow)

This: http://www.dailymotion.com/video/x1smw4q_part-1-of-the-pf-tek-instruction-video-making-the-substrate-for-magic-mushrooms_tech

Edited by esore (02/11/15 06:44 PM)

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OfflinetaGyo
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Re: My first grow attempt failed to colonize. [Re: esore]
    #21260210 - 02/11/15 06:48 PM (9 years, 3 months ago)

You're missing the point. The lid is plenty protection, the foil prevents GE.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlineesore
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Registered: 10/08/14
Posts: 22
Last seen: 8 years, 9 months
Re: My first grow attempt failed to colonize. [Re: taGyo]
    #21260245 - 02/11/15 06:53 PM (9 years, 3 months ago)

Quote:

taGyo said:
You're missing the point. The lid is plenty protection, the foil prevents GE.




So the jar on the left in that picture is good then?


What I meant was the the jar on the right had no lid under the tin foil - the tin foil layer replaced the lid. The no lid method I got from this, but it doesn't really matter since for my new batch I used lids.
http://puu.sh/fNGB5/8564cb54cd.jpg

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OfflinetaGyo
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Re: My first grow attempt failed to colonize. [Re: esore]
    #21260272 - 02/11/15 06:56 PM (9 years, 3 months ago)

Jar on the left is how you want all your jars to look.

That video is dumb, use a lid all the time.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlineesore
Stranger
Registered: 10/08/14
Posts: 22
Last seen: 8 years, 9 months
Re: My first grow attempt failed to colonize. [Re: taGyo]
    #21260296 - 02/11/15 06:59 PM (9 years, 3 months ago)

Quote:

taGyo said:
Jar on the left is how you want all your jars to look.

That video is dumb, use a lid all the time.




Alright thanks

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OfflinetaGyo
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Re: My first grow attempt failed to colonize. [Re: esore]
    #21260303 - 02/11/15 07:01 PM (9 years, 3 months ago)

Sorry,

I just spent my whole day working on my car, if you have questions message me, I'm generally a lot nicer.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlineesore
Stranger
Registered: 10/08/14
Posts: 22
Last seen: 8 years, 9 months
Re: My first grow attempt failed to colonize. [Re: taGyo]
    #21260531 - 02/11/15 07:41 PM (9 years, 3 months ago)

Shit, I just opened 4 of my old jars (Not the one with growth) and threw the contents out. I just realized the jars are slightly tapered at the top. I'm so pissed, they were labelled as untapered. It's a tiny little ridge so I didn't notice earlier, but it made it impossible to remove the contents without breaking it up. If my new batch colonizes should I break up the mycelium to remove it, or try smashing the jar with a hammer? I already inoculated :frown:


ALSO! The verm in the 3 jars was totally clean. No mold, no smell, nothing. It was just a chunk of verm. The 4th jar (Jar B) was definitely moldy so I just put it in garbage. No point even reusing the other jars I guess since they're tapered

Edited by esore (02/11/15 07:54 PM)

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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
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Last seen: 3 years, 3 days
Re: My first grow attempt failed to colonize. [Re: esore]
    #21260595 - 02/11/15 07:54 PM (9 years, 3 months ago)

A fully colonized and consolidated cake will shrink a bit.

Those don't really look any different than ball regular mouth 1/2 pints so I'll bet when you get a cake done proper you'll have no trouble getting it out


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

Extras: Filter Print Post Top
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