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OfflineMKultra44
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Advice on bacteria contamination
    #21258660 - 02/11/15 01:40 PM (9 years, 3 months ago)

Iam fairly new to growing and Ive run into a problem. I set up my incubator in my bathroom with a little space heater to make sure the temperature stays at a constant 80 degrees. Well the other day before my gf went to work she cranked up the heater so she wouldnt be cold when she got out the shower. When I got home it was 103 degrees, It was heartbreaking cus I was sure it fucked something up. I had 6 spawn bags two were 90% colonized 2 were about 75% and the other 2 were around 60-70%. Yesterday when I checked all the bags were excreting metabolites and the two least colonized were kind of slimy and reaked so I tossed them. So here comes the part were I need advice Iam in the process of trying to save the remaining four bags. The two 90% bags smelt half shroomy half offish smell(cant put a name on it) so I just broke them up and placed them back in my incubator with the lid off and room temperature. Iam hoping there able to reestablish there networks and fight of any bacteria. The other 2 didnt really smell right at all, so I dunked them in hydrogen peroxide last night until they stopped bubbling, discarded all the lose uncolonized grains and h2o2 then dunked them in ice water over night. So when I get off work today I plan on trying to spawn the 2 I dunked lastnight into a couple of my monotubs. Iam I just being a yahoo for trying to save them? or is there some hope?

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OfflineBugler Boy
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Re: Advice on bacteria contamination [Re: MKultra44]
    #21258686 - 02/11/15 01:48 PM (9 years, 3 months ago)

Scrap the incubator and 80+ degree colonization temps or alls you're going to be growing is bacteria. Room temp is fine

Sounds like everything is scrapped at this point, I'd start over. 103 is pretty close to thermal death.

Why ice water for dunking? Cubes are a tropical species (assuming thats what your growing)


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The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

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OfflineMandarinfish

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Re: Advice on bacteria contamination [Re: MKultra44]
    #21258691 - 02/11/15 01:49 PM (9 years, 3 months ago)

.

Edited by Mandarinfish (07/25/20 11:27 PM)

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OfflineGroo
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Re: Advice on bacteria contamination [Re: MKultra44]
    #21258701 - 02/11/15 01:52 PM (9 years, 3 months ago)

Being where you were a long time ago. I didn't feel like a yahoo for doing what you are doing. Now after a long time of learning and what not I still do what you do. Not so much for the desperation of imminent failure but to observe the raging war and to see who wins the ensuing battle. You are absolutely not crazy and it is perfectly human to do what you are doing. People may put you down. But its cuz thay are jealous :goodluckwiththat2: LOL Okay they are not jealous but you will learn patience or you will move on to another hobby simple as that..

Once you smell the funk chances are it will not win.

Edited by Groo (02/11/15 01:54 PM)

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Offlinesaintburger
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Re: Advice on bacteria contamination [Re: Groo]
    #21258719 - 02/11/15 01:55 PM (9 years, 3 months ago)

These guys are right. Incubators don't serve much of a purpose in Mycology, if any. BTW I like your username MKultra


--------------------
Set goals for yourself, or wander around aimlessly.

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and saw a Cheshsire cat in a tree.
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'Where do you want to go?' was his response
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'"

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OfflineMKultra44
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Re: Advice on bacteria contamination [Re: saintburger]
    #21259328 - 02/11/15 03:59 PM (9 years, 3 months ago)

Thanks guys, yea after doing more research Iam starting to see incubators are outdated. which Is good because i've been keeping my shroom stuff all over the house trying to keep two different temperatures for colonizing and fruiting lol now I can consolidate everything into one awesome shroom room. The ice water is kind of a experiment, I figured what ever grew in there did so in really hot conditions so they might hate a colder climate. I know theyre a tropical species but i've heard of people putting cakes in the fridge or cold water to shock them into pinning so I figured they could take it. So know instead of putting all my efforts into a lost cause Iam going to go make a bad ass set up in my guest bathroom, Iam still going to throw the spawn in a monotub but mainly just to see if what I did had any effect. If I start to smell anything weird Iam throwing it out though

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OfflineMKultra44
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Re: Advice on bacteria contamination [Re: saintburger]
    #21259355 - 02/11/15 04:05 PM (9 years, 3 months ago)

Thanks, Project MKultra holds a special place in my heart because without it the counterculture of the 60s might have never happened lol

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OfflineMKultra44
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Re: Advice on bacteria contamination [Re: MKultra44]
    #21431497 - 03/19/15 02:25 PM (9 years, 2 months ago)

Quick update! I actually salvaged one of the spawn bags that i dunked in hydrogen peroxide. I got 2 flushes from it around 4 ounces dried altogether. Also salvaged one of the other bags that I shook up and let recolonize, its working on its 3rd flush right now. I also made a dope little shroom room in my guest bathroom where Iam letting half my jars colonize in room temperature and the other half colonize in a TIT setup with a aquarium heater, I wana see which method is more successful for my setup. Thank for all the help guys, cant believe my last ditch efforts actually worked sometimes it pays to experiment.

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Offlinemushpunx
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Re: Advice on bacteria contamination [Re: MKultra44]
    #21431631 - 03/19/15 02:52 PM (9 years, 2 months ago)

The TIT incubator is the way to go IMO, deoending on how much spawn you have. Sometimes my house gets real cold and I have to bust one out, I use two of them

Next time I would advise against the peroxide dude but nice job pulling a QP from it, certainly nothing to scoff at!

Btw, what is room temp in your house? On average?


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OfflineMKultra44
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Re: Advice on bacteria contamination [Re: mushpunx]
    #21513435 - 04/07/15 02:07 AM (9 years, 1 month ago)

The room temp in my house usually stays around 74. The 12 jars I have in my TIT stay around 79 and so far they've colonized faster than the ones I left out. So the advantage does go to the TIT but Ill prolly keep the half and half combo going so I can have jars ready at different time periods

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InvisibleTheEaglesGift
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Re: Advice on bacteria contamination [Re: MKultra44]
    #21513528 - 04/07/15 03:23 AM (9 years, 1 month ago)

TiTs can be dangerous. Fish tank heaters are prone to breaking for no good reason, but the heating element can stay functioning and set your shit on fire.

I've been in the tropical fish hobbies for 20+ years. I've seen heaters bust for absolutely no reason more times than you can imagine. They're oddly unreliable.

TiTs scare me for that reason... be safe folks.

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InvisibleInocuole
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Re: Advice on bacteria contamination [Re: TheEaglesGift]
    #21513562 - 04/07/15 03:41 AM (9 years, 1 month ago)

Quote:

TheEaglesGift said:
TiTs can be dangerous. Fish tank heaters are prone to breaking for no good reason, but the heating element can stay functioning and set your shit on fire.

I've been in the tropical fish hobbies for 20+ years. I've seen heaters bust for absolutely no reason more times than you can imagine. They're oddly unreliable.

TiTs scare me for that reason... be safe folks.




I feel like this is something that should be more common knowledge with how many new people we have coming in here every day using these things.  Thanks for sharing that.


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OfflineSockadin
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Re: Advice on bacteria contamination [Re: Inocuole]
    #21513662 - 04/07/15 04:40 AM (9 years, 1 month ago)

http://www.fungifun.org/English/Pftek

From the link on the main board. If you click the Growing Mushrooms; Gettings Started; PF Tek Tab it will lead you to that website above. It actually talks about the importance of using a TIT. We need to change the information on the main page if we are going to get anywhere with changing the culture.

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InvisibleTheEaglesGift
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Registered: 04/10/11
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Re: Advice on bacteria contamination [Re: Inocuole]
    #21513666 - 04/07/15 04:43 AM (9 years, 1 month ago)

Quote:

Inocuole said:
Quote:

TheEaglesGift said:
TiTs can be dangerous. Fish tank heaters are prone to breaking for no good reason, but the heating element can stay functioning and set your shit on fire.

I've been in the tropical fish hobbies for 20+ years. I've seen heaters bust for absolutely no reason more times than you can imagine. They're oddly unreliable.

TiTs scare me for that reason... be safe folks.




I feel like this is something that should be more common knowledge with how many new people we have coming in here every day using these things.  Thanks for sharing that.



My pleasure brother. :thumbup:

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Offlinemushpunx
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Re: Advice on bacteria contamination [Re: TheEaglesGift]
    #21514155 - 04/07/15 09:28 AM (9 years, 1 month ago)

Ive got a fish tank heater horror story haha

I didnt realize I left the heater in a pile of appliances by the printer, that happened to have tons of clothes piled on top... So when my girlfriend asked me to plug in the printer I didn't think anything of it and accidentally plugged in the fish tank heater instead of the printer

3 am we smell smoke cant find the source, figured its coming from the walls called the firemen to see if they could send someone by to check the house. Ugh.. we got a whole truck full of firemen stomping through the house it was humiliatimg when they walked out wirh a tank heater and a burnt Tshirt hahaha

Good thing it wasnt a real fire before I even called I was running around frantic moving tubs and jars out to the garage


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OfflineMycologist217
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Re: Advice on bacteria contamination [Re: mushpunx]
    #21514351 - 04/07/15 10:21 AM (9 years, 1 month ago)

Your bathroom is the worst room to keep colonizing cultures in. Every time you flush your toilet your sending flatulents into the air, not to mention to the humid nature of them anyway. I'm surprised no one said this already, but anywhere else would be a better place to store them.


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