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apsous
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Registered: 01/16/15
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Last seen: 9 years, 2 days
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Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. *DELETED*
#21252478 - 02/10/15 09:17 AM (9 years, 3 months ago) |
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Post deleted by apsousReason for deletion: It already exists.
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21252516 - 02/10/15 09:29 AM (9 years, 3 months ago) |
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15,000lbs of substrate down the shitter? That is a truly epic failure. Might go back to the drawing board...
It's hard to say what your problem was exactly but using old spawn is a sure fire way to fail.
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Humbled
Local Budtender




Registered: 01/19/08
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: drake89]
#21252545 - 02/10/15 09:36 AM (9 years, 3 months ago) |
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A center temp that swings from 86-96 F is pushing the limits for incubation temperatures in my opinion. Contaminants are starting to be favored at such high temperatures.
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apsous
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: drake89]
#21252561 - 02/10/15 09:41 AM (9 years, 3 months ago) |
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Quote:
drake89 said: It's hard to say what your problem was exactly but using old spawn is a sure fire way to fail.
I'm suggesting to re-pasteurize it in order not to through it away.
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apsous
Stranger

Registered: 01/16/15
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: Humbled]
#21252574 - 02/10/15 09:44 AM (9 years, 3 months ago) |
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Quote:
Psilocelium said: A center temp that swings from 86-96 F is pushing the limits for incubation temperatures in my opinion. Contaminants are starting to be favored at such high temperatures.
They apppeared after 3-4 days... And most of them are in the surface, they didn't started from the center (pasteurization failure). Speaking logically, tight packing or high temperature are the reasons but I need experienced opinions. What about the fact that the Spawn was 10 days expired?
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21252621 - 02/10/15 10:01 AM (9 years, 3 months ago) |
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Quote:
apsous said:
Quote:
drake89 said: It's hard to say what your problem was exactly but using old spawn is a sure fire way to fail.
I'm suggesting to re-pasteurize it in order not to through it away.
You want to re use contaminated substrate? Bad idea.
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apsous
Stranger

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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: drake89]
#21252912 - 02/10/15 11:22 AM (9 years, 3 months ago) |
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Quote:
You want to re use contaminated substrate? Bad idea.
Is it? It really doesn't change a lot... I just want to ask Temperature and Time (D and Z values) for Trichoderma. It is a petty to through away this straw... Sterilization is another option as well... The most important is to figure out the reason of this problem...Then the rest.
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Yerow
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Registered: 09/22/14
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21253005 - 02/10/15 11:51 AM (9 years, 3 months ago) |
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You made 2 threads? Meh. People have already told you what you are doing wrong, but you seem to ignore it.
Very bad idea to reuse contaminated substrate Very bad idea to buy colonized grains from anywhere, make your own. Very bad idea to keep temps so high
And all i told you in the other thread. 
Room temperature for colonizing most mushroom species, go to 28c, 30c and more and you are promoting contamination. No idea what degrees are in fahrenheit.
Edited by Yerow (02/10/15 11:55 AM)
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apsous
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: Yerow]
#21253144 - 02/10/15 12:29 PM (9 years, 3 months ago) |
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Quote:
Yerow said: You made 2 threads? Meh. People have already told you what you are doing wrong, but you seem to ignore it.
Very bad idea to reuse contaminated substrate Very bad idea to buy colonized grains from anywhere, make your own. Very bad idea to keep temps so high
And all i told you in the other thread. 
Room temperature for colonizing most mushroom species, go to 28c, 30c and more and you are promoting contamination. No idea what degrees are in fahrenheit.
This is the second thread since I realized that this is the Community of Pleurotus (Gourmet mushroom).
I would gladly delete the previous one, please tell me how (new in this forum). Just keep in mind that, even if this is the right place to post (Gourmet) about Pleurotus Ostreatus, I got the solution from you at the Mushroom Cultivation forum. Take under consideration that it is harder for me to follow 2 different posts at the same time and also keep in mind that I lost lots of Euros so far, so please judge gently for reposting at this forum... I wanted to find the source... Not to fool arround. Honestly.
Edited by apsous (02/10/15 12:31 PM)
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21253165 - 02/10/15 12:36 PM (9 years, 3 months ago) |
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a lot of things could have happened to make your bags go green. I'm guessing more than one thing went wrong- your spawn certainly wasn't good and your bags may have been under pasteurized or under aerated. If they were that hot they you did something very wrong. Why not develop a method you know works before going bankrupt?
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21253178 - 02/10/15 12:39 PM (9 years, 3 months ago) |
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It's all good dude. I think your big problem is using straw. Trich loves straw. Trich doesn't love sawdust, but pleurotus does. I think if you switch to pasteurized sawdust it will eliminate your trich problem no matter where you get your spawn. Coffee and straw are great, nutritious substrates, but you need to be 100% on point with your pasteurization or they will trich out
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (02/10/15 12:40 PM)
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: NumeroEno]
#21253235 - 02/10/15 12:53 PM (9 years, 3 months ago) |
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Quote:
NumeroEno said: It's all good dude. I think your big problem is using straw. Trich loves straw. Trich doesn't love sawdust, but pleurotus does. I think if you switch to pasteurized sawdust it will eliminate your trich problem no matter where you get your spawn. Coffee and straw are great, nutritious substrates, but you need to be 100% on point with your pasteurization or they will trich out
Oysters will outgrow trich on straw if your methods are correct. Using bad spawn is simply foolish. My pinks take a week on straw and 3 weeks on sawdust at about the same spawn rate. All straw is different.
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solarity
mm... my favourite food



Registered: 03/31/09
Posts: 1,590
Loc: UK
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21253247 - 02/10/15 12:55 PM (9 years, 3 months ago) |
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"32C (86F) to 36C" - that is your first problem right there. Unless you are growing pink oysters that is thermal death.
Old spawn is a possible issue, depends on the spawn rate too. I wasted 100+ bags over old spawn.
You now have a very mouldy incubation area so it will need a very thorough clean before you try again. Effectively you just packed wet straw into a nice warm environment and the mould came in via the air holes with nothing to stop it.
-------------------- Commercial exotics farmer for 8 years - now sold up!
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drake89
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Registered: 06/26/11
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: solarity]
#21253273 - 02/10/15 01:00 PM (9 years, 3 months ago) |
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I have not had bags get that hot without supplements. Do you think the mold caused the temp rise?
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Yerow
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Registered: 09/22/14
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: solarity]
#21253282 - 02/10/15 01:01 PM (9 years, 3 months ago) |
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apsous
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Registered: 01/16/15
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: solarity]
#21253342 - 02/10/15 01:21 PM (9 years, 3 months ago) |
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Quote:
solarity said: "32C (86F) to 36C" - that is your first problem right there. Unless you are growing pink oysters that is thermal death.
It's Pleurotus ostreatus so I guess is what you mentioned. Do you think expire date plays a role in incubation? I know spawn should be produced by me BUT this is a second job for me and no time to make spawn run for the moment.
Quote:
solarity said:Old spawn is a possible issue, depends on the spawn rate too. I wasted 100+ bags over old spawn.
i'll make trials because most of the bags are clean (only surface contamination) inside (no growth of anything). I need info about temperature and time of death in Trichoderma though...
Quote:
solarity said:You now have a very mouldy incubation area so it will need a very thorough clean before you try again.
Of course. pest control is critical at this point as well.
Quote:
solarity said:Effectively you just packed wet straw into a nice warm environment and the mould came in via the air holes with nothing to stop it.
Well in the same area other bags were colonized succefully. I don't think that's the main problem.
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apsous
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: drake89]
#21253397 - 02/10/15 01:41 PM (9 years, 3 months ago) |
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Quote:
drake89 said:
Quote:
NumeroEno said: It's all good dude. I think your big problem is using straw. Trich loves straw. Trich doesn't love sawdust, but pleurotus does. I think if you switch to pasteurized sawdust it will eliminate your trich problem no matter where you get your spawn. Coffee and straw are great, nutritious substrates, but you need to be 100% on point with your pasteurization or they will trich out
Oysters will outgrow trich on straw if your methods are correct. Using bad spawn is simply foolish. My pinks take a week on straw and 3 weeks on sawdust at about the same spawn rate. All straw is different.
In some cases Mycelium killed Trich, that's true...But to be safe, I need kiling information for Trich. temperature and time to kill them....
What changes in sawdust ? How you pasteurize it?
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apsous
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Registered: 01/16/15
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: drake89]
#21253414 - 02/10/15 01:47 PM (9 years, 3 months ago) |
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Quote:
drake89 said: I have not had bags get that hot without supplements. Do you think the mold caused the temp rise?
I saw that 3-5 days after spawning the temperature of the center is rising too high (30C/86F). This and the fact that the bag was packed tight, could cause low temperature exchange and the center couldn't cool down fast enough, causing mycelium death is my guessing... Plus the fact that the center is free from any other contamination make me believe that pasteurization was successful.... The only alternative explanation is the the spawn I used which was expired (10 days) was too weak to develop... But I don't think so...
Edited by apsous (02/10/15 02:29 PM)
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apsous
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Registered: 01/16/15
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21267665 - 02/13/15 05:56 AM (9 years, 3 months ago) |
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Good morning mushroom people. Here are some photos from a new batch that I spawned at 09/02/2015. I stored the bags in different temperatures. One is room temperature 25C/77F and the other 22C/71,6F.
First the 25C room temperature:


Then the 22C:


It seems that the incubation at 25C is more aggressive than the one 22C. BUT at the 25C the center temperature of the bags was 30-31C (86-88F). In the other 2 bags the center temperature of the bags is 27-28C (80-82F).
Is it possible to have a heat death in the inner of the first bags?
In this photos you will see that the bag is totally clean without any contamination and without any mycelium as well. 
Can it be the high center temperature a possible explanation to this (no mycelium growth at all)?
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mcchieftan
Part man, part mushroom



Registered: 09/08/05
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Re: Grow Bag (mycobag) Incubation Problems for Oyster Mushrooms and Mycobag conditions information. [Re: apsous]
#21267672 - 02/13/15 06:00 AM (9 years, 3 months ago) |
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Er.. can you stop updating both threads and just leave one? My OCD is twitching out...
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