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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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P. Galindoi question
#21249885 - 02/09/15 06:14 PM (9 years, 11 months ago) |
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How long does it take Galindoi spores to germinate? I have some on PDA, grain and BRF. Yes I know the BRF is wildly inappropriate for its typical purpose;I just wanted to knock up a couple cakes for actual fruiting to print sooner, and brf is pretty hard to fuck up. This is my first time with this variety, so if anyone has any favorite threads or teks they want to link, that would be great too!
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: P. Galindoi question [Re: Asric]
#21249964 - 02/09/15 06:24 PM (9 years, 11 months ago) |
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Hamloaf had some on agar recently. I think it depends more on condition of your agar andcondition of your spores.
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AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Re: P. Galindoi question [Re: Grey]
#21250153 - 02/09/15 06:54 PM (9 years, 11 months ago) |
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Ambient temp for the petris is 70F. I use Hetlik's grocery store agar tek. The spores came from a vendor and were shipped while outdoor temps were in the 30s and 40s.
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: P. Galindoi question [Re: Asric]
#21250180 - 02/09/15 06:59 PM (9 years, 11 months ago) |
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is your agar really moist? I made some up, and its looks like kinda like dried wax. I'm waiting on some spores to germinate. I'll probably up the water a little more next time. When did you put the spores to them?
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Re: P. Galindoi question [Re: Grey]
#21250208 - 02/09/15 07:05 PM (9 years, 11 months ago) |
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Agar is pretty moist. The agar has a slightly glossy look. Most of the petris have just a little condensation even. I cloned some oysters the other week on the same recipe, and they took a little bit of time to recover then went crazy on it. It has been just over 5 days. I am by no means worried yet, just excited and curious.
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: P. Galindoi question [Re: Asric]
#21250229 - 02/09/15 07:09 PM (9 years, 11 months ago) |
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could be 3 days could be 3 weeks
start a lot of plates is my advice
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: P. Galindoi question [Re: Asric]
#21250242 - 02/09/15 07:11 PM (9 years, 11 months ago) |
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Okay, I'm sure you'll be fine then. I swiped mine about the same time, and I'm really trying not to ogle them all day. They're cubes not galindoi though, I'd be more excited if they were though.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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FungusOfTheBungus


Registered: 02/05/15
Posts: 121
Last seen: 8 years, 7 months
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Re: P. Galindoi question [Re: Asric]
#21250435 - 02/09/15 07:46 PM (9 years, 11 months ago) |
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I ordered a print of P. Galindoi last month. Made some syringes and inoculated 10 rye grain jars. It took about 5 days before I spotted signs of germination. This species seems to be an overall slower colonizer than cubensis.
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BUK
Stranger


Registered: 11/03/14
Posts: 60
Last seen: 3 years, 1 month
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Slow to germinate but once it's going it's very fast - G2G to fully colonised in three days
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: P. Galindoi question [Re: BUK]
#21252731 - 02/10/15 10:32 AM (9 years, 11 months ago) |
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Also found it to be a fast colonizer.
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Waiting for this is like waiting on my first grow all over again.
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: P. Galindoi question [Re: Asric]
#21253001 - 02/10/15 11:49 AM (9 years, 11 months ago) |
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I get that man. Just now turning over into the stone producers in a few days when I can do li. Gonna be very exciting. Check out a thread by aero on stones, it's amazing. I'm only a few weeks ahead of you now, looking at stack of colonized mex/a and galindoi plates a couple transfers from spores. A beautiful sight keep at it...
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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That thread by aero is sick. If I could get one of those cultures...
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AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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FungusOfTheBungus


Registered: 02/05/15
Posts: 121
Last seen: 8 years, 7 months
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Re: P. Galindoi question [Re: Grey]
#21253618 - 02/10/15 02:32 PM (9 years, 11 months ago) |
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I'm jealous. My jars have been a bit on the slow side. I shook them last night. Maybe that will help.
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: P. Galindoi question [Re: Asric]
#21253767 - 02/10/15 03:02 PM (9 years, 11 months ago) |
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--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Re: P. Galindoi question [Re: Grey]
#21255057 - 02/10/15 07:15 PM (9 years, 11 months ago) |
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That is pretty bad ass. If I clone a stone, do big stones typically yield more mass per quart of grain or should I look for a monoculture that produces large numbers of smaller ones?
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: P. Galindoi question [Re: Asric]
#21255179 - 02/10/15 07:35 PM (9 years, 11 months ago) |
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It probably depends on the isolate I would imagine, but for what it is worth devilstone had a massive stone setup and his favorite isolate that delivered the most weight produced "pea" or "chickpea" sized stones.
Check it out.
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Re: P. Galindoi question [Re: d0urd3n]
#21255585 - 02/10/15 08:45 PM (9 years, 11 months ago) |
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Wicked. Strain isolation here I come. When ingclassy asked me how long ago I put these to agar, It hadn't really hit me how short it's been. I am not seeing any contamination in any of the 17 petris though, so that is a good sign, I guess.
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: P. Galindoi question [Re: Asric]
#21255615 - 02/10/15 08:50 PM (9 years, 11 months ago) |
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now leaving contam city 
check out user una's threads, too
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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suspekt33
Sclerotia Enthusiast



Registered: 08/23/13
Posts: 22
Loc: Outer Heaven (Seychelles)
Last seen: 9 days, 10 hours
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Re: P. Galindoi question [Re: Asric]
#21280954 - 02/16/15 01:03 AM (9 years, 10 months ago) |
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Hi there Sir did you get germination? I received my Galindoi print from the approved vendors two months ago of course you know a Galindoi print is tiny. So I decided to try my hand at agar so that I can conserve my print. I made up some agar plates using baby food jars. I dropped some spores onto the agar exactly one week ago I had to toss out a few jars because a few contaminated. However I do have a few which I think have germinated spores. I can see where the black dots are (dry spores) where I dropped the spores. And about 4 days after knocking up the agar it went "gelly" around the knock points. Then it became fuzzy around them. Now I've got little cotton ball puffs they dont seem to be growing across the plate like I expected. But then again this wasn't mycelium to agar. This was a dry spore to Agar. I have scoured the internet to get a picture so that I can compare them with what I sould he seeing but to no avail. I will upload pic as soon as possible, could this be due to my agar recipe? Mois (I have to setup my SAB first) I thought its maybe cobweb mold but I really have no clue. Secondly if they are proper spore germinations and not contams. How do I transfer to another plate? Thank you in advances guy. Peace.
Edited by suspekt33 (02/17/15 12:01 AM)
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Asric
Fueled By Satan



Registered: 06/18/07
Posts: 247
Last seen: 9 years, 5 months
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Re: P. Galindoi question [Re: suspekt33]
#21283106 - 02/16/15 02:07 PM (9 years, 10 months ago) |
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No germination on any of the things I tried. Agar, WBS,BRF all still showing no growth. Well a couple of the petri's eventually contaminated, but most are still sitting still with no activity. :/
-------------------- Looking to trade for:
Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R.
Prints of: Z strain, Treasure Coast, Exotics, Morels
Have to trade:
Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane
Prints: B+
Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
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suspekt33
Sclerotia Enthusiast



Registered: 08/23/13
Posts: 22
Loc: Outer Heaven (Seychelles)
Last seen: 9 days, 10 hours
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Re: P. Galindoi question [Re: Asric]
#21283290 - 02/16/15 02:42 PM (9 years, 10 months ago) |
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Thanks for your reply. I've been researching the entire day. I just had a quick squizz at paul stamets mushroom cultivator for edible and medicinal mushrooms. There's a small chapter about germinating spores on agar plates. Ideally I should have streaked them in an "S" shape however I just scraped them using a sterile needle and dropped onto the plate. It's been just over a week and I'm not seeing contamination. Except the pure white cotton balls. If I look below I see the black spore spot. I assume that if this is not mycelium but instead contamination then it means either my noc tool. Or my spores were dirty? If this is mycelium what am I transferring? Where are the sectors? Or do I transfer a piece of the cotton balls onto another agar plate and then wait for Rhizo growth? Thanks in advance folks. P.S this is my first attempt at this hobby. I'm skipping PF tek because, supplies are not easy to come by where I'm from. I've prepped myself over the last 18 months to perform agar and grain (rye) teks. I could do PF but then I'd need to get tapered mason jars. More verm and BRF. Again thanks.
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What does a man do Walter? A man provides for his family. And a man, a man provides. And he does it even when he's not appreciated, or respected, or even loved. He simply bears up and he does it. Because he's a man. - Gustavo Fring
The Fallen Shall Rise Again
This man is my Idol:
Stonesuns Epic Sclerotia Tek!
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: P. Galindoi question [Re: suspekt33]
#21283377 - 02/16/15 02:59 PM (9 years, 10 months ago) |
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Just wait a bit longer. My spots germinated, and some of the plates still have that cotton ball look. If I hold it up to the light at an angle I can see a matte look on the agar thats shaped like rhizo strands. My other plates had that, then quickly grew thick mycelium in those places.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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theblacksheep


Registered: 01/08/14
Posts: 294
Last seen: 8 years, 9 months
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Re: P. Galindoi question [Re: Grey]
#21283815 - 02/16/15 04:22 PM (9 years, 10 months ago) |
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I have brf jars of atl7 inoculated last night with liquid culture made from agar.
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 3 years, 3 months
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Hey suspekt33 don't say the name of the seller when talking about cultivation. We buy spores for MICROSCOPY.
-------------------- Cluster Headache sufferer? Cluster Busting
Veil Tear GIF
Flower Pot Grow GIF
Mini Mono Tub GIFS
"All mushrooms are edible, but some only once."
-- Croatian Proverb
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suspekt33
Sclerotia Enthusiast



Registered: 08/23/13
Posts: 22
Loc: Outer Heaven (Seychelles)
Last seen: 9 days, 10 hours
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Re: P. Galindoi question [Re: urthtown]
#21286212 - 02/17/15 12:37 AM (9 years, 10 months ago) |
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Sorry about that. I've edited my post. Will keep in mind for future posts.
--------------------
What does a man do Walter? A man provides for his family. And a man, a man provides. And he does it even when he's not appreciated, or respected, or even loved. He simply bears up and he does it. Because he's a man. - Gustavo Fring
The Fallen Shall Rise Again
This man is my Idol:
Stonesuns Epic Sclerotia Tek!
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