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OfflineSpinScratch
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Will I run into Senescence?
    #21246925 - 02/09/15 06:52 AM (9 years, 3 months ago)

I inoculated 1 quart WBS jars with MS syringe. I expanded that jar into 10 jars. Then with those 10 jars I made 2 mono tubs. Then I cloned a fruit from one of those mono tubs and after a few agar transfers, I have an isolate.

If I took an agar wedge of this isolate and inoculated a WBS jar, and then expanded that into 10 jars, and maybe even expand some of those again, am I going to see issues with senescence? also, if I took a spore print from this project would that "reset" the senescence issue? Thanks

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Offlinemushpunx
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Re: Will I run into Senescence? [Re: SpinScratch]
    #21246940 - 02/09/15 06:59 AM (9 years, 3 months ago)

If you took a spore print it will no longer be an isolate, you have yo grow from a live culture to keep that going.

As long as you inoculate your master jar with a piece grown out from your isolate culture you shouldn't have any problems unless you G2G out too many times but I think 2 generations out is ok yea?

Store your isolate in a slant tube and when you want to make grain masters inoculate a new plate with a tiny piece. If you make a new slant every couple of years you can keep your culture alive for ages.

Senescence.. if I understand the term correctly is more likely to happen if you G2G out too many generations. Ive never gone past 2 I have always just started a new master jar and gone from there.
If you store your cultures correctly it should be easy enough to avoid.


Nice dude! So you isolated a single sector from your clone yea?




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Edited by mushpunx (02/09/15 07:04 AM)

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OfflineSpinScratch
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Re: Will I run into Senescence? [Re: mushpunx]
    #21246962 - 02/09/15 07:14 AM (9 years, 3 months ago)

Quote:

mushpunx said:



Nice dude! So you isolated a single sector from your clone yea?






well... maybe, i think so lol

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Offlinemushpunx
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Re: Will I run into Senescence? [Re: SpinScratch]
    #21246991 - 02/09/15 07:23 AM (9 years, 3 months ago)

Quote:

SpinScratch said:
Quote:

mushpunx said:



Nice dude! So you isolated a single sector from your clone yea?






well... maybe, i think so lol





Haha

A clone is still made up of a few strains, an isolate is a single strain (mono culture). If you take a clone and clean it up with a few transfers, it is usually still a clone.

If you cut away the sectors and grow them out, each one of those would be an isolate. Im still learning how to do this and identify once I have one

Clones rule man Im on a clone hunt myself this week!


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