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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Rye Soak Water Liquid Culture! [Re: taGyo]
    #21243539 - 02/08/15 12:19 PM (9 years, 11 months ago)

Thanks for answering my quetions Ham. You are the man. :bow2:


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Invisibleshroominmyroom
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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
    #21243904 - 02/08/15 01:24 PM (9 years, 11 months ago)

:flyhigh:

Edited by shroominmyroom (12/05/17 12:47 PM)

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Invisibleeatyualive
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
    #21244435 - 02/08/15 03:47 PM (9 years, 11 months ago)

Quote:

hamloaf said:
Yessir.  I documented the making of my WBS GWLC media a few pages back.  In fact a few posts up thread I made 5 WBS GWLC.  2 are quarts.  One is in pint, and the other 2 WBS GWLC's are in gallon sized media vessels.  ALL with magnetic stirring capabilities. 

The gallons and quart that were sterilized at the same time like 4 days ago have been inocualted with blue Oyster LC. 

The 3 WBS GWLC that were inoculated last week are colonizing and have been stirred with the magnets a couple of days ago.  Again, that action is documented on the last page or so.  Stirbar action and all.



thanks. ill reread it!


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Invisibled0urd3n
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Re: Rye Soak Water Liquid Culture! [Re: eatyualive]
    #21245403 - 02/08/15 07:54 PM (9 years, 11 months ago)

Ehh. I'm a bit worried about how it is looking. See that thing floating to the right? I hope it isn't bacteria what do you guys think? (click them to expand)







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OfflinetaGyo
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
    #21245701 - 02/08/15 09:02 PM (9 years, 11 months ago)

Ew,
I think it's bacteria.


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InvisibleGrey
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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
    #21245868 - 02/08/15 09:48 PM (9 years, 11 months ago)

Looks like my kombucha forming a new scoby.


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Invisibled0urd3n
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
    #21245975 - 02/08/15 10:23 PM (9 years, 11 months ago)

That is funny. Exactly what I thought. I was thinking recently I would like to try and get another scoby growing. I just can't make it taste as well as it is in the store. :mad:


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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
    #21246042 - 02/08/15 10:39 PM (9 years, 11 months ago)

Yeah, I just recently ran into problems with mine.



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Invisibled0urd3n
Just call me "D"

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Re: Rye Soak Water Liquid Culture! [Re: Grey]
    #21246050 - 02/08/15 10:41 PM (9 years, 11 months ago)

Damn I would completely start over with that for sure. That is odd they normally don't have issues. You are using cheesecloth or a thin shirt over the top right?

Sorry this is kind of off topic. :lol:

I think there is a big kombucha thread in the Pub or somewhere. We should revive it.


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InvisibleGrey
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
    #21246062 - 02/08/15 10:45 PM (9 years, 11 months ago)

Coffee filter. Yeah, I had another going separately.

Yeah, I don't wanna derail this thread


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Invisibled0urd3n
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
    #21248104 - 02/09/15 12:40 PM (9 years, 11 months ago)

I think it is just mycelium actually. It is pan myc I think that is why it looks a bit different. I have about 1/4 inch growing off the agar wedge and after swirling and waiting a day or two I see it spread across the top. It looks the same as what is growing off the wedge. I think its just a mycelial mat. I have no clue how long to leave it before testing. Any ideas guys? Do you guys have a general time frame you use? I imagine it varies with the species. Being pretty dark it is hard to tell how much exactly is growing in there. I wish I would have diluted it a bit. It seemed to get darker after pressure cooking.


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OfflinehamloafM
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
    #21249651 - 02/09/15 05:33 PM (9 years, 11 months ago)

Quote:

As for my plan for this LC and all the others I am making, I would rather not say right now. I am working on something that requires a liquid form of inoculate and much testing is required before I post it.



Bah, don't keep the cat in the bag, Pasty.  Share with the community what you are researching and developing on.  You never know, somebody may see something you don't, be able to translate that to you, and your R & D will prosper as a result.  Nobody'd going to steal your ideas.

Quote:

man ham i really want to try this, but i never soak my grain lol.


 
eatyu, you could make an exception to never soaking your grains just once to collect some end of the boil-cycle grain water.  Maybe like ten cups of grain, or so.  A little bit of Grain water, undiluted, goes a long way.  Especially if you dilute to 4%.  Diluting to 4% is where I would recommend folks just getting started with the end of the boil-cycle grain water liquid culturing media technique begin.  The end of the boil-cycle grain water can be placed into the freezer for storage.

Quote:

Oak woodchip soak water would probably work though



Oak, or any hardwoods' end of the boil-cycle woodchip water will work as a culturing medium for woodlover species over dung loving species like Cubensis. 

I actually collected me a gallon of undiluted hard woodchip (apple, hickory, cherry, and pecan) end of the boil-cycle water to utilize as a culturing media for my next run of Pan Stip (glow in the dark bitty oysters) liquid cultures. 



Quote:

I always just give my culture a good shake, never needed stir bars or any of that,



How do you know that you've "never needed stir bars or any of that,", if you've never tried them??  Magnetic stirring capabilities with LC's are HANDS DOWN the BEST way to, without use of your hands, shake a liquid culturing medium.  More liquid medium can be fit into a single media vessel due to the hands free shaking of a liquid culture magnetic stirring capabilities allow the cultivator while simultaneously eliminating any threat of getting your filtering material wet during the "shaking" of an LC.  Furthermore, using magnetic stirplates and stirbars generates a MUCH denser, more concentrated mycelial culture than shaking by hand could ever result in, thus making more efficient use, biologically, out of the media, and culture.  Moral of the story; don't knock it until you've tried it. 

Quote:

Ehh. I'm a bit worried about how it is looking. See that thing floating to the right? I hope it isn't bacteria what do you guys think? (click them to expand)




I wouldn't be concerned about writing that culture off as contaminated just yet for that growth doesn't look like bacteria to me, moreover, that growth looks like mycelial culture that's floated to the top and is beginning to colonize the top of the media.  If the culture is left to colonize the top of the media like that, it will suffocate and kill what ever culture is underneath the mycalial mat.

The major thing I see wrong with your media so far is the damn finger prints all over the outside of your media vessel.  Handling your liquid media vessels without plastic gloved, alcohol soaked hands is a HUGE vector for contamination. 

What did you say (if you even said) you inoculated your LC with, and, what temperature range would you say your LC's are incubating @??


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OfflinetaGyo
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
    #21249687 - 02/09/15 05:39 PM (9 years, 11 months ago)

I use LCs very rarely to be honest with you. I'm big on LI at the moment along with A2G. The only reason I'm really looking into grain water LCs is to both expand my knowledge and for a couple upcoming projects that will need tons of liquid media.

I'm not knocking stir bars or marbles or glass, I just personally don't use them because a shake has worked for me in the past. Will this change? Probably. When I first started growing I thought 25 dollars for 25 pounds of rye was a good deal.

Things change, along with growing methods. I'm not NEARLY on the same playing field as you, Ham, or Pasty so I'm just trying to touch everything I think is worth it as I dial in my own method.


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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
    #21249776 - 02/09/15 05:55 PM (9 years, 11 months ago)

Took the 3 most recently sterilized rye liquid mediums that were diluted to 4%, and inoculated them each with a single wedge of live culture from a petri dish using the "wedge and transfer" technique of atl#7.   

Nice, clean ATL#7 culture on sterilized, nutrified agar in a petri dish.  :cool:


Agar wedge to 3, freshly sterilized, nutrified liquid mediums in a wide mouth pint jars filled with 500ml's of liquid media each.  :awesome:
   

Here goes the Red Milo inoculated with the undiluted Red Milo GWLC five days from inoculation colonizing away.

 

Happy, glossy white mycelial culture is again observed.  :super:

Here goes a look at WBS GWLC's.

Pint jar LC 5 days after being magnetically stirred.  :laugh:
 

Quart jar media colonizing away after the 5th day of being stirred magnetically.  :thumbup:


The culture is DENSE and thickening up every hour.  Another blast from the magnetic stirring devices will cause this culture to shred and settle upon it's self condensing/concentrating the liquid mycelial culture into a super dense, high-powered media broth.


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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
    #21250163 - 02/09/15 06:56 PM (9 years, 11 months ago)

Wow that is fast Hamloaf!

Quote:

hamloaf said:

Quote:

Ehh. I'm a bit worried about how it is looking. See that thing floating to the right? I hope it isn't bacteria what do you guys think? (click them to expand)




I wouldn't be concerned about writing that culture off as contaminated just yet for that growth doesn't look like bacteria to me, moreover, that growth looks like mycelial culture that's floated to the top and is beginning to colonize the top of the media.  If the culture is left to colonize the top of the media like that, it will suffocate and kill what ever culture is underneath the mycalial mat.

The major thing I see wrong with your media so far is the damn finger prints all over the outside of your media vessel.  Handling your liquid media vessels without plastic gloved, alcohol soaked hands is a HUGE vector for contamination. 

What did you say (if you even said) you inoculated your LC with, and, what temperature range would you say your LC's are incubating @??




It is Pan myc. Yeah I think you are right. I looked at it last night. And after a light swirl the day before it seems to be myc on the top. It looks exactly like what is growing directly outwards from the wedge. Mine is a lot slower than yours seem to be going. I imagine because I am not using a magnetic stirrer and can't shake it. I guess I will wait a few weeks and see what she looks like.


Why does it matter if I touch it without gloves? I just have it sitting on a shelf. Do you not touch your grain jars without ISO'd gloves? I don't see how there would be a difference as long as the filter and lid are working fine?


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InvisiblePastywhyteMDiscord
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
    #21250319 - 02/09/15 07:24 PM (9 years, 11 months ago)

Quote:

hamloaf said:
Quote:

As for my plan for this LC and all the others I am making, I would rather not say right now. I am working on something that requires a liquid form of inoculate and much testing is required before I post it.



Bah, don't keep the cat in the bag, Pasty.  Share with the community what you are researching and developing on.  You never know, somebody may see something you don't, be able to translate that to you, and your R & D will prosper as a result.  Nobody'd going to steal your ideas.




I will share but just not yet. I have only just harvested from the initial prototype and so many changes have happened since then that to release it now would be far too premature. This will either be an epic fail or will change the way noobs enter the hobby and the way veterans look at growing as well.

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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
    #21250335 - 02/09/15 07:27 PM (9 years, 11 months ago)

Damn, now I really wanna know.


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OfflinetaGyo
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
    #21250339 - 02/09/15 07:28 PM (9 years, 11 months ago)

Quote:

ingclassy said:
Damn, now I really wanna know.




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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
    #21252104 - 02/10/15 07:12 AM (9 years, 11 months ago)

Quote:

Pastywhyte said:
Quote:

hamloaf said:
Quote:

As for my plan for this LC and all the others I am making, I would rather not say right now. I am working on something that requires a liquid form of inoculate and much testing is required before I post it.



Bah, don't keep the cat in the bag, Pasty.  Share with the community what you are researching and developing on.  You never know, somebody may see something you don't, be able to translate that to you, and your R & D will prosper as a result.  Nobody'd going to steal your ideas.




I will share but just not yet. I have only just harvested from the initial prototype and so many changes have happened since then that to release it now would be far too premature. This will either be an epic fail or will change the way noobs enter the hobby and the way veterans look at growing as well.



What's that?  Inoculating bulk substrate materials with super liberal amounts of LC?

I am going to throw those PESH cultures on the stirplate today, and stir them up.  Get them all dense with culture.  Hope that inoculation gun comes in today.  That would be cool. 

Good luck with your research and developments.  I understand not want to share your work until you are done researching and developing though.  I have a few things in the works that I'd rather finish before posting about. 

Good luck with your research.  Hope everything works out for you, and a new approach to mushroom cultivation is culminated as a result.  Onward and upward!


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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
    #21253590 - 02/10/15 02:27 PM (9 years, 11 months ago)

Quote:

d0urd3n said:
Wow that is fast Hamloaf!

It is Pan myc. Yeah I think you are right. I looked at it last night. And after a light swirl the day before it seems to be myc on the top. It looks exactly like what is growing directly outwards from the wedge. Mine is a lot slower than yours seem to be going. I imagine because I am not using a magnetic stirrer and can't shake it. I guess I will wait a few weeks and see what she looks like.


Why does it matter if I touch it without gloves? I just have it sitting on a shelf. Do you not touch your grain jars without ISO'd gloves? I don't see how there would be a difference as long as the filter and lid are working fine?



I do not ever touch ANY of my media vessels with out iso'ed, gloved hands!  My media vessels incubate inside of a makeshift, poormans' sterile laboratory, so basically, my media vessels aren't handled unless I am showered, wearing freshly laundered scrubs, and the flowhood has been running for about 2 hours.  My media vessels don't even have any dust particles on them and squeak when you touch them. 

The reason why it matters whether or not you touch your media vessels with gloved hands is because your hands are LADENED with ALL kinds of bacteria and mold spores.  If you were to put those finger prints under a microscope there would be billions of contamination spores in the shape of your finger print.  If you were to open a petri dish containing nutrified, sterilized agar, and pressed your bare finger into the media, in just a few days there would be a contamination outbreak in the shape of your finger print. 

All that contamination remaining on the outside of your media vessels like that can easily be wicked into the sterilized media resulting in infected spawn.


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