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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
WBS sprouting after sterilization
    #21244831 - 02/08/15 05:28 PM (9 years, 3 months ago)



Hey guys
So I soaked my wbs for 24 hrs and ligjtly simmered before loading up my jars and sterilizing at 15 psi for 90 minutes. I inoculated 12 hours later. The white film is kn the outside of the jar and this pic was taken on day 2 after inoculation.

How did this even happen? Has anyone seen anything like this?


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Edited by JvF (02/08/15 05:33 PM)

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: WBS sprouting after sterilization [Re: JvF]
    #21244858 - 02/08/15 05:33 PM (9 years, 3 months ago)

Are you absolutely sure it didn't happen during the soak?  Mark the tip of the root and see if it's growing.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: WBS sprouting after sterilization [Re: Psilicon]
    #21244911 - 02/08/15 05:44 PM (9 years, 3 months ago)

some seeds can survive a sterilization event but probably not any of those seeds, my guess is it happened during the soak.

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OfflineTheMustardTiger
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Registered: 08/19/13
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Re: WBS sprouting after sterilization [Re: bodhisatta]
    #21244934 - 02/08/15 05:48 PM (9 years, 3 months ago)

Definitely sprouted during the soak.


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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: WBS sprouting after sterilization [Re: TheMustardTiger]
    #21244957 - 02/08/15 05:52 PM (9 years, 3 months ago)

Shit.

so these jars are pretty much screwed?


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InvisiblebodhisattaMDiscordReddit
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Re: WBS sprouting after sterilization [Re: JvF]
    #21244964 - 02/08/15 05:53 PM (9 years, 3 months ago)

not necessarily

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: WBS sprouting after sterilization [Re: JvF]
    #21244966 - 02/08/15 05:54 PM (9 years, 3 months ago)

No, they're probably just fine.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineTheMustardTiger
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Registered: 08/19/13
Posts: 4,700
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Last seen: 6 years, 8 months
Re: WBS sprouting after sterilization [Re: Psilicon]
    #21244971 - 02/08/15 05:55 PM (9 years, 3 months ago)

I doubt it will continue to grow. The Mycelium won't bat an eye. Should be fine.


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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: WBS sprouting after sterilization [Re: TheMustardTiger]
    #21245295 - 02/08/15 07:24 PM (9 years, 3 months ago)

Good stuff guys, thanks for the input. Was feeling pretty shitty about my first attempt:blush:


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