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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 13 years, 7 months
Whats the least ammount of innoculant I can use?
    #2124078 - 11/20/03 12:34 AM (13 years, 8 months ago)

Whats the least amount of spore solution youve used in quart grain jars and had good success? I want to keep it to a minimum so I dont have to depend on liquid myc/g2g transfers in order to wind up with the same number of jars. Less transfers mean contams are less likely.

Im going with ralphsters so ill have potent syringes, none of that practicaly clear bs. Also, how many dark syringes can you make off of a spore print typically?


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Invisiblez@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: Whats the least ammount of innoculant I can use? [Re: stoner2002]
    #2124110 - 11/20/03 01:02 AM (13 years, 8 months ago)

I use about 1/2 a cc per quart jar of birdseed from a dar syringe. I usually get 6-10 syringes from a print depending on how the size and quality of the print.


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 13 years, 7 months
Re: Whats the least ammount of innoculant I can use? [Re: z@z.com]
    #2124182 - 11/20/03 02:01 AM (13 years, 8 months ago)

1/2 a cc per quart? Are you shittin me? That put a huge smile on my face.

Do you notice that huge a difference between innoculating with 1/2cc and say 3ccs? Id imagine it would only be a few days...


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Offlinefidget
Fungus Hunter

Registered: 03/18/02
Posts: 245
Loc: Oregon
Last seen: 11 years, 8 months
Re: Whats the least ammount of innoculant I can use? [Re: stoner2002]
    #2124237 - 11/20/03 02:49 AM (13 years, 8 months ago)

It only takes a bit. The more you use the quicker the colonization, but i have used less than 1/2 a cc and got a fully colinized jar.


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- fidget

"With ordinary consciousness you can't even begin to know what's happening."



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Invisibleralphster44
collector
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Registered: 01/04/01
Posts: 4,657
Re: Whats the least ammount of innoculant I can use? [Re: stoner2002]
    #2124745 - 11/20/03 11:44 AM (13 years, 8 months ago)

Just use 1 cc...squirt it all over.
Works for me:)

And, thanks for the syringe compliment:)

Ralphster44


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Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
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Re: Whats the least ammount of innoculant I can use? [Re: ralphster44]
    #2124907 - 11/20/03 01:14 PM (13 years, 8 months ago)

IMO it's worth it to put at least 1 cc, sometimes two for quick colonization, if you're making your own syringes it's no big deal if ordering from a vendor, then you may want to be more sparing, depends on what kind of project you're working on.


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InvisibleKlingonFromUranus
enthusiast

Registered: 05/03/03
Posts: 488
Loc: Los Angeles
Re: Whats the least ammount of innoculant I can use? [Re: thisone]
    #2125896 - 11/20/03 09:18 PM (13 years, 8 months ago)

agreed. One can get away with using less, but the more one uses, the more likely the spores haven't been wasted. If you get a dark syringe, you can cut it many times over. Put 1 or 2 cc's of dark fluid into a separate syringe and take the syringe with 1 or 2 cc's of dark solution in it and draw up the rest of the syringe with sterile water. If the original solution was dark, you will still be able to see some of the spores floating around......or so I've heard :wink:


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OfflinefIsh in my head
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Registered: 01/25/03
Posts: 1,132
Loc: 4500 ASL
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Re: Whats the least ammount of innoculant I can use? [Re: KlingonFromUranus]
    #2125939 - 11/20/03 09:34 PM (13 years, 8 months ago)


Really not much needed. I once prepared 17 pf half-pint with a single not really dark 10 cc syringes.

It was still a fast colonization.

.fs.


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Offlineaje
Why not?
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Registered: 10/11/03
Posts: 16,392
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Re: Whats the least ammount of innoculant I can use? [Re: fIsh in my head]
    #2125951 - 11/20/03 09:39 PM (13 years, 8 months ago)

most people use something between .5 - 2 cc's


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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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