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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: MudaFuka]
    #21206479 - 01/31/15 11:25 AM (9 years, 3 months ago)

Scofield is boss. Guy in the 2nd pic is Miles Davis


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OfflineEdmunter
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Munchauzen]
    #21207347 - 01/31/15 03:29 PM (9 years, 3 months ago)

Im trying to thank you but this video sums up all my feelings.  Just think 'Munchauzen ' instead Bret

Munchauzen! youve got it going on!


Edited by Edmunter (01/31/15 06:38 PM)

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OfflinetaGyo
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Edmunter]
    #21208021 - 01/31/15 06:10 PM (9 years, 3 months ago)

:lmafo:


--------------------
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OfflineEdmunter
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: taGyo]
    #21219512 - 02/03/15 04:08 AM (9 years, 3 months ago)

Do you have a vid for taking spore prints, then adding to a solution?  I reccon you should journal this with an index, its just the best thing on the web.

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OfflinetaGyo
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Edmunter]
    #21219684 - 02/03/15 06:34 AM (9 years, 3 months ago)



You can ascertain the steps from here, just don't iso your needle or your cup. Wrap your shot glass in foil and PC it. I bring my PC to my SAB and then open it real quick and load everything into my clean SAB via the arm holes. Then I get the print, scrape after flame sterilizing my scraper and get them in the shot glass (After taking off the foil being really careful not to put my finger inside).

Then shoot the water, suck, shoot, suck, shoot, etc.

Munch you should update this though.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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InvisibleMunchauzen
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: taGyo]
    #21220023 - 02/03/15 08:53 AM (9 years, 3 months ago)

RR has a video for syringes but I'll make one when I got some caps to print

http://www.mushroomvideos.com/Sporeprinting-Syringes

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Offlineinvitro

Registered: 05/03/13
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Munchauzen]
    #21221063 - 02/03/15 01:04 PM (9 years, 3 months ago)

:takingnotes:

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Offlineqwobbler
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Munchauzen]
    #21233845 - 02/06/15 05:04 AM (9 years, 3 months ago)

For the " Easy inoculation w/o belender tek"....

the water in the jar he puts it in, is that sterile water from a PC? or just standard water? or LC?

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InvisibleMunchauzen
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: qwobbler] * 1
    #21234092 - 02/06/15 07:21 AM (9 years, 3 months ago)

Sterile tap water that I PCed. Don't use more than a pint of water per plate or it can get too diluted

Edited by Munchauzen (02/06/15 07:22 AM)

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Offlineqwobbler
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Re: Mushroom Cultivation Video Series (Updated w/Agar & Pasty Plate Prep!) [Re: Munchauzen]
    #21236372 - 02/06/15 06:07 PM (9 years, 3 months ago)

Quote:

Munchauzen said:
Sterile tap water that I PCed. Don't use more than a pint of water per plate or it can get too diluted




Cheers mate

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Invisiblestareatclouds
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Re: SAB Sterile Tek Video Series [Re: qwobbler]
    #21237380 - 02/06/15 10:22 PM (9 years, 3 months ago)

I just watched the agar to agar transfer. Shouldn't you cool the blade in the receiving end since it's much more likely to be clean? The plate with the growth could have something bad on it.

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InvisibleMunchauzen
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Re: SAB Sterile Tek Video Series [Re: stareatclouds]
    #21237440 - 02/06/15 10:41 PM (9 years, 3 months ago)

IDK. I'm sure it is debatable, though. One counter argument to that might be that you are moving around a cooled blade in a moderately clean environment more by going back and forth between the plates. I have honestly never had any problems doing this, though. Contaminates on my transfer plates are very rare.

Edited by Munchauzen (02/06/15 10:41 PM)

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InvisibleMudaFuka
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Re: SAB Sterile Tek Video Series [Re: Munchauzen] * 1
    #21237455 - 02/06/15 10:46 PM (9 years, 3 months ago)

When I make a transfer. I don't even bother cooling the blade. I just start cutting out my wedge while the blade is still hot. You singe the first bit of mycelium but you leave enough healthy mycelium to start growth on the new plate.


--------------------
AMU
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InvisibleMunchauzen
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Re: SAB Sterile Tek Video Series [Re: MudaFuka]
    #21237467 - 02/06/15 10:50 PM (9 years, 3 months ago)

well there ya go :thumbup:

Edited by Munchauzen (02/06/15 10:56 PM)

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Invisiblestareatclouds
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
    #21237484 - 02/06/15 10:54 PM (9 years, 3 months ago)

Probably. I have concerns about everything, heh. I've just always read/heard that to avoid contams, you'd dip into the receiving plate since it's cleaner. I think RR was an advocate of that. Either way, I'm sure your contam rate is pretty low so I doubt it's that much of a risk, if any. Awesome vids, I like them a lot.

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InvisibleMudaFuka
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
    #21237496 - 02/06/15 10:55 PM (9 years, 3 months ago)

The way you do it seems fine to me too. If there were conams on the portion of the plate where you are cooling your blade you would see them growing. That's sort of the point of agar.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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InvisibleMunchauzen
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Re: SAB Sterile Tek Video Series [Re: MudaFuka]
    #21237510 - 02/06/15 10:59 PM (9 years, 3 months ago)

Quote:

MudaFuka said:
The way you do it seems fine to me too. If there were conams on the portion of the plate where you are cooling your blade you would see them growing. That's sort of the point of agar.




that is what I always thought. just use my :eyeball::eyeball:

Edited by Munchauzen (02/06/15 10:59 PM)

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Invisibleelasticaltiger
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Re: SAB Sterile Tek Video Series [Re: MudaFuka] * 1
    #21240856 - 02/07/15 07:07 PM (9 years, 3 months ago)

Quote:

MudaFuka said:
When I make a transfer. I don't even bother cooling the blade. I just start cutting out my wedge while the blade is still hot. You singe the first bit of mycelium but you leave enough healthy mycelium to start growth on the new plate.




:whathesaid:


--------------------
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InvisiblebodhisattaMDiscordReddit
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Re: SAB Sterile Tek Video Series [Re: elasticaltiger]
    #21240864 - 02/07/15 07:08 PM (9 years, 3 months ago)

Quote:

elasticaltiger said:
Quote:

MudaFuka said:
When I make a transfer. I don't even bother cooling the blade. I just start cutting out my wedge while the blade is still hot. You singe the first bit of mycelium but you leave enough healthy mycelium to start growth on the new plate.




:whathesaid:



:whathesaid:

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InvisiblebodhisattaMDiscordReddit
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Re: SAB Sterile Tek Video Series [Re: bodhisatta]
    #21240867 - 02/07/15 07:09 PM (9 years, 3 months ago)




I didn't cool the blade for that transfer, everything worked out just fine. the second picture is the same thing zoomed in

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   OlympusMyco.com Olympus Myco Grow Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract


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