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storm eagle
Efilnikufesin



Registered: 11/10/14
Posts: 221
Loc: Far beyond the sun
Last seen: 4 years, 5 months
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How many times can you g2g the g2g..
#21233922 - 02/06/15 05:47 AM (9 years, 3 months ago) |
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I mean.. I g2g from my first wbs jar to 9 jars of oats. Can I take one of the wbs/oat jars and g2g again when it's ready? If so.. how long can you keep this up? I'd be g2g the wbs/oats > oats
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Edited by storm eagle (02/06/15 05:48 AM)
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Albertheisenhouer
Tryptamine doctor



Registered: 10/26/13
Posts: 734
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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21233927 - 02/06/15 05:48 AM (9 years, 3 months ago) |
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I have done 4 G2G's and it still works fine. Some people advise to do it until the 3rd.
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Stromrider
This must be the place



Registered: 06/02/13
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4 or 5 times
Take those first 9 jars and stick 8 of them in the fridge. Use the one you have left to g2g to jars you're going to fruit. Each time you're ready to g2g again just whip one of your original 9 out of the fridge.
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storm eagle
Efilnikufesin



Registered: 11/10/14
Posts: 221
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Cool man. I feel this is probably the best way to do penis envy since they don't drop spores really. My agar must be too stiff because I havnt even had viable contams after 2 weeks so I've g2g a bunch lately.
I was gonna spawn 7 of em. G2g one of em to another 10 jars I have ready and then refill the 7 plus a few extra I have laying around With the other.
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storm eagle
Efilnikufesin



Registered: 11/10/14
Posts: 221
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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21233971 - 02/06/15 06:09 AM (9 years, 3 months ago) |
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How long are they good in the fridge. I'm using polyfil lids. Should I wrap em in foil first If I decide to try this?
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chucklehead
GeneticThrowback toNeanderthal



Registered: 02/17/07
Posts: 1,338
Loc: Chucky Cheese
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Re: How many times can you g2g the g2g.. [Re: Stromrider]
#21233980 - 02/06/15 06:13 AM (9 years, 3 months ago) |
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I'm on the side of the 3 transfers for a total of 4 generations. I find after that is where trouble begins. The health of the mycelium had declined to the point that you will start to see less yield and smaller fruits. I've done up to 9 generations before just to see what happened. I don't recommend it. The material is prone to contamination, the fruiting is week and the fruits are unhealthy. I advise no more than 3 transfers and 4 generations. Honestly if you keep a set of master cultures on Agar of your favorites, this 4 generation method can go on a very long time. Just remember to transfer your master cultures every now and then to fresh slants.
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Albertheisenhouer
Tryptamine doctor



Registered: 10/26/13
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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21233994 - 02/06/15 06:19 AM (9 years, 3 months ago) |
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I have used colonised rye which was left in the fridge for half a year without problems. Agar plates can dry out after a while. For better storage you can use cardboard in test tubes. when stored cardboard properly, you can save a culture for years.
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Albertheisenhouer
Tryptamine doctor



Registered: 10/26/13
Posts: 734
Loc: Holland
Last seen: 22 days, 18 hours
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There is a good chapter about storage in "Psilocybin mushroom handbook" (2006) from l.G. Nicholas and Kerry Ogame. (downloadable, if you're a pirate)
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TheMultiverse44
StickyLemonFarmer


Registered: 05/02/14
Posts: 147
Last seen: 8 years, 8 months
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Re: How many times can you g2g the g2g.. [Re: Stromrider]
#21234019 - 02/06/15 06:28 AM (9 years, 3 months ago) |
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Quote:
Stromrider said: 4 or 5 times
Take those first 9 jars and stick 8 of them in the fridge. Use the one you have left to g2g to jars you're going to fruit. Each time you're ready to g2g again just whip one of your original 9 out of the fridge.
thissssss!!!
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---->Looking for Psilocybe Atlantis spore prints<----
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storm eagle
Efilnikufesin



Registered: 11/10/14
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Thanks all for all this info. I'm over here with 26 jars total colonizing. 3 separate masters. Just the first batch of 9 are almost done. I broke one master jar trying to break it up on my couch, would of been the 4 set of g2g and my first mono isn't even full colonized yet. Shit was a mess.. so now Im going to replace it with one of my g2g jars From a first transfer Since I have jars ready From the break.
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Albertheisenhouer
Tryptamine doctor



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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21234028 - 02/06/15 06:33 AM (9 years, 3 months ago) |
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I don't know if you are already doing this, but you should write certain things on everything you use.
On my spawn I write: species code, something like B+ and then a number refering to the master culture. Date of inoculation number of transfers performed
I do this since I am testing several different races of cubensis
On my tubs I write: species code substrate ratios date of inoculation
This helps me organising quite a bit, especially when experimenting with different substrates and different races of cubies
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storm eagle
Efilnikufesin



Registered: 11/10/14
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I know to label em like that but I don't for now only because I'm rain maning it until I'm overwhelmed. Probably start that soon..
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TheMultiverse44
StickyLemonFarmer


Registered: 05/02/14
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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21235622 - 02/06/15 03:03 PM (9 years, 3 months ago) |
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Everything looks great!!! Your G2G technique is on point!!!!
Also i like that Black table
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---->Looking for Psilocybe Atlantis spore prints<----
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storm eagle
Efilnikufesin



Registered: 11/10/14
Posts: 221
Loc: Far beyond the sun
Last seen: 4 years, 5 months
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The black table? Target. A.k.a. the mycology supercenter.
I'm glad my g2g is on point. Still waiting to put my first mono into fruiting.. any day now..
Those almost colonized jars will be a week old tomorrow.
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bw86
Doesn't play well with others


Registered: 11/12/06
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Re: How many times can you g2g the g2g.. [Re: storm eagle]
#21235818 - 02/06/15 03:49 PM (9 years, 3 months ago) |
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i have gone dozens of generation of g2g and saw no decline in vigor or fruit size.
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cronicr



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Loc: Van Isle
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Re: How many times can you g2g the g2g.. [Re: bw86]
#21235837 - 02/06/15 03:54 PM (9 years, 3 months ago) |
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Quote:
bw86 said: i have gone dozens of generation of g2g and saw no decline in vigor or fruit size.
ms or isolates?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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fishy1
strangest


Registered: 07/15/04
Posts: 443
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Re: How many times can you g2g the g2g.. [Re: bw86]
#21235941 - 02/06/15 04:15 PM (9 years, 3 months ago) |
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Quote:
bw86 said: i have gone dozens of generation of g2g and saw no decline in vigor or fruit size.
Same here. From MS.. no problems, no difference.
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bw86
Doesn't play well with others


Registered: 11/12/06
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Re: How many times can you g2g the g2g.. [Re: fishy1]
#21235955 - 02/06/15 04:19 PM (9 years, 3 months ago) |
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MS and pin clone, the pin clone i only remember going about 6 gens but i didnt stop using it for any reason in particular just moved on.
Edited by bw86 (02/06/15 04:23 PM)
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storm eagle
Efilnikufesin



Registered: 11/10/14
Posts: 221
Loc: Far beyond the sun
Last seen: 4 years, 5 months
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Re: How many times can you g2g the g2g.. [Re: bw86]
#21236593 - 02/06/15 06:54 PM (9 years, 3 months ago) |
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I should wait till its at 100 before I fridge it? Wrap it in foil For the fridge? How long should it thaw out or come to room temp before use?
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chucklehead
GeneticThrowback toNeanderthal



Registered: 02/17/07
Posts: 1,338
Loc: Chucky Cheese
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Re: How many times can you g2g the g2g.. [Re: bw86]
#21236775 - 02/06/15 07:34 PM (9 years, 3 months ago) |
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Actually dozens? That's pretty cool. I was never so lucky. I'd be interested in getting my hands on those genetics.
I ran one particular clone for several years and by the 6th generation you could usually tell things were going sideways to the tune of about 25% off in yield and 5% in vitality. Later generations showed greater decline. I reasoned that if at gen 6 I was seeing consistently diminishing returns I was probably just dismissing previous loss random variations. So I backed it up a couple generations and that's where I do my restart back to a wedge. I tried running a few other clones out to later generations some started to show marked decline at later generations than others.
I think MS actually gets better in the jar after a few generations but in the tub the results vary in my experience. That's why we clone and isolate after all.
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