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InvisibleBig Bear
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Re: Pasty Agar Tek [Re: Big Bear]
    #21224702 - 02/04/15 07:39 AM (9 years, 21 days ago)



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OfflineEdmunter
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Re: Pasty Agar Tek [Re: Jon2jay]
    #21224916 - 02/04/15 09:02 AM (9 years, 21 days ago)

Quote:

Jon2jay said:
How are you guys getting minimal condensation?! :/

How can I make liquid inoculation using a pasty plate? I thought I found a tek somewhere, but cannot find it. I believe it just involved squirting sterile water into the plate and vigorously mixing it and voila!

Any help on this?




Watch the videos in my signature its in there. How to use a SAB


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Invisiblebudmanman
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Re: Pasty Agar Tek [Re: Edmunter]
    #21225361 - 02/04/15 11:01 AM (9 years, 21 days ago)

Alright so my mini rounds are colonizing nicely. The hole had to be made larger for the tyvek my first round in the pc all crushed from the cool down so I re made some with larger tyvek breather holes, much much larger and everything went smooth.


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Invisibleelasticaltiger
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Re: Pasty Agar Tek [Re: Edmunter]
    #21228003 - 02/04/15 08:56 PM (9 years, 20 days ago)

Quote:

Edmunter said:
I just wanted to say after all the problems ive been having with my agar mix and speed  and definition of growth.  Violet was right.  That grain water agar is the absolute nuts.  Everything grows super quick and thick with great definition........ Im feeling slightly aroused......



:heytheresexy:


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OfflineEdmunter
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Re: Pasty Agar Tek [Re: elasticaltiger]
    #21229096 - 02/05/15 02:38 AM (9 years, 20 days ago)

Quote:

elasticaltiger said:
Quote:

Edmunter said:
I just wanted to say after all the problems ive been having with my agar mix and speed  and definition of growth.  Violet was right.  That grain water agar is the absolute nuts.  Everything grows super quick and thick with great definition........ Im feeling slightly aroused......



:heytheresexy:



:idhitit:


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Offliner00tuuu123
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Re: Pasty Agar Tek [Re: Big Bear]
    #21229460 - 02/05/15 07:15 AM (9 years, 20 days ago)

Quote:

Big Bear said:

2.  Absolutely.  Drop a plate in some sterile water and grind that shit up.  There are lots of methods.  Munch made a simple one in his mushroom cultivation videos series.  Also Muda has a great LI tek with other ways to do it.  The world is your King Oyster



I was thinking I could pc my blender base and pc some water jars then dump my plate in the water(actually grain water) then blend it up and re lid them with new pcd lids, Iknow it is one too many steps/vectors but what do you guys think?


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: r00tuuu123]
    #21229475 - 02/05/15 07:28 AM (9 years, 20 days ago)

That sounds like absolute hell to deal with. You should just buy an Oster blender and a regular mouth 1/2 pint jar and you're set. Seriously, it's like $20 tops and about 353757x easier to deal with.

PC distilled water in 1/2 pint with blender blade inside jar. Put in SAB to cool. Slice out agar in SAB and drop into 1/2 pint blender jar. Blend and inoculate whatever.

It's that easy. :smile:


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OfflineJon2jay
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Re: Pasty Agar Tek [Re: stareatclouds]
    #21230043 - 02/05/15 11:07 AM (9 years, 20 days ago)

You guys are awesome. And fyi my pastyplates stay with condensation all through colonizing. Oh well, my hats off to mister pasty for this tek because I was able to get my feet wet with agar.. oh how sweet it is!


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Anything posted by me is either hypothetical or completely fictional and is for entertainment only.


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OfflineEdmunter
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Re: Pasty Agar Tek [Re: Jon2jay]
    #21230053 - 02/05/15 11:10 AM (9 years, 20 days ago)

The trick is to shake and tip out the excess condensation at/just befor  inoculation.


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OfflinePsyCLown89
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Re: Pasty Agar Tek [Re: r00tuuu123]
    #21230157 - 02/05/15 11:43 AM (9 years, 20 days ago)

Quote:

r00tuuu123 said:
Quote:

Big Bear said:

2.  Absolutely.  Drop a plate in some sterile water and grind that shit up.  There are lots of methods.  Munch made a simple one in his mushroom cultivation videos series.  Also Muda has a great LI tek with other ways to do it.  The world is your King Oyster



I was thinking I could pc my blender base and pc some water jars then dump my plate in the water(actually grain water) then blend it up and re lid them with new pcd lids, Iknow it is one too many steps/vectors but what do you guys think?




So sort of like a LI / LC sort of thing? Grain water would just offer extra nutrients which isn't really needed for a LI, however if you plan on making a LC then sure. Unless I am missing something?

Personally I am not too sure whether that is worth it really. From what I have heard and seen (never tried a proper LI yet myself) it colonizes quick enough.
If you had to wait for the myc to colonize further in the LC slurry it would just be time wasted which could have gone to the myc colonizing the jars. :shrug:



So after being unable to find a suitable and reasonably priced blender I decided to order an Oster ice crusher blade assembly. Then I will silicone it to one of my jar lids and use a drill with a square drill bit piece as the motor to blend the cakes. Hope it work properly!


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: Jon2jay]
    #21230869 - 02/05/15 02:31 PM (9 years, 19 days ago)

Quote:

Jon2jay said:
You guys are awesome. And fyi my pastyplates stay with condensation all through colonizing. Oh well, my hats off to mister pasty for this tek because I was able to get my feet wet with agar.. oh how sweet it is!



I stack my pasty plates in a 27qt tote at room temperature for about a week before I use them. By the time I use them there is no condensation.


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: MudaFuka]
    #21230897 - 02/05/15 02:37 PM (9 years, 19 days ago)

Stacking during colonization is fine right?

I haven't for fear of contams/GE issues.


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: taGyo]
    #21230911 - 02/05/15 02:39 PM (9 years, 19 days ago)

yah stacking is fine. If I couldn't stack my plates, they would take up every shelf and counter top in my house.


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: MudaFuka]
    #21230920 - 02/05/15 02:41 PM (9 years, 19 days ago)

:lol:,

Got it. Thanks Muda. Whenever I think I'll hit a contam doing something I ask you and you're like "Yeah, definitely done that."

Respect mayne,

:thumbup:


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: MudaFuka]
    #21231053 - 02/05/15 03:11 PM (9 years, 19 days ago)

Quote:

MudaFuka said:
I stack my pasty plates in a 27qt tote at room temperature for about a week before I use them. By the time I use them there is no condensation.




Do you have to dump out water pooling on the inside?


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: stareatclouds]
    #21231081 - 02/05/15 03:17 PM (9 years, 19 days ago)

No


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Offliner00tuuu123
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Re: Pasty Agar Tek [Re: stareatclouds]
    #21231086 - 02/05/15 03:18 PM (9 years, 19 days ago)

Quote:

stareatclouds said:

PC distilled water in 1/2 pint with blender blade inside jar. Put in SAB to cool. Slice out agar in SAB and drop into 1/2 pint blender jar. Blend and inoculate whatever.

It's that easy. :smile:


That's what I was gonna do my blender isn't an Oster but the base fits small mouth jars. The only extra step would be the new PC'd lids.


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: r00tuuu123]
    #21231121 - 02/05/15 03:26 PM (9 years, 19 days ago)

I've started putting SFD lids on some of my 1/2 pint LI jars for storage after blending. I haven't been doing it long enough to know how long they can be stored for but it should work out OK.


--------------------
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OfflineJon2jay
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Re: Pasty Agar Tek [Re: MudaFuka]
    #21233131 - 02/05/15 10:35 PM (9 years, 19 days ago)

is it strange im not seeing ANY contams in my pasty plates? I made a batch of 8, inoculated 2 with grains, did 6 transfers, and no contams in anything? I find it hard to believe.. lol

The condensation is a pain in the ass though, it is potentially the fluctuation in temp in the room. The winter really screws any steady temperatures up.


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: Jon2jay]
    #21233137 - 02/05/15 10:38 PM (9 years, 19 days ago)

all I have seen is bacteria, which is from a contaminated syringe. have never seen mold on a plate.

good sterile tek will get you far


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