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Offlinefearandloathingden
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Registered: 01/21/15
Posts: 17
Loc: Colorado Flag
Last seen: 9 years, 10 months
Germination
    #21228975 - 02/05/15 01:11 AM (9 years, 11 months ago)

Okay, so I know patience is key in this hobby. That being said... I inoculated my brf jars 5 and 1/2 days ago with Cambodian spores from a syringe from the internet. I PC'd my jars half for an hour and half for 90 min and let cool over night. Gave them each about 1 cc per jar, I've had them sitting on top of my closet in the dark the temperature varies between 70 and 80 degrees, I would have made an incubator but this site told me they were not needed. I wouldn't ask this question yet normally but all that i have read is that Cambodians are usually pretty fast growing.

Why am i seeing no signs of growth at all?
If I was dealing with contams would i still see growth?
Am I just being impatient?

Also I bought the PC new and it told me to put some oil around the gasket and after Sterilizing i noticed oily water and oil residue on the outside of the jars, I didn't really wipe it off so I could avoid contams. It was canola oil, I don't think any got in but i don't want to leave any info out in-case that could also be a problem

Edited by fearandloathingden (02/07/15 04:04 PM)

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InvisibleToadstool5
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Re: Germination [Re: fearandloathingden]
    #21228994 - 02/05/15 01:25 AM (9 years, 11 months ago)

I would give it more time before worrying.

I have had golden teacher take over 2 weeks to show growth, sometimes its miserable. Almost makes it difficult to sleep at night lol

Did you order the syringe or make it from a print? If you made the syringe without letting the spores re-hydrate first then it will sometimes stall.

Did you cover the jars with foil when you PC them? Incorrect water level and forgetting foil can make the cakes too wet. Compressing the mixture too much in the jar and not maintaining a fluffy aerated texture can cause stalling as well.

Got gas exchange? :grin:

The canola oil shouldn't have caused a problem with foil over the jars.

In my experience you do see contams as the mycellium starts so if you dont see anything at all or slime then there's a good chance you are being impatient .

:goodluck:

Edited by Toadstool5 (02/05/15 01:28 AM)

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Offlinefearandloathingden
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Re: Germination [Re: Toadstool5]
    #21229001 - 02/05/15 01:31 AM (9 years, 11 months ago)

I covered with foil and left the foil on until the moment I inoculated and I did order the spores, There was a big purple cloud of spores, so I    sucked in sterile air to make a bubble so I could shake them up good. Also I followed the 2-1-1 recipe for my Jars.

And thank you for the Input!!!

Edited by fearandloathingden (02/05/15 01:34 AM)

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InvisibleToadstool5
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Re: Germination [Re: fearandloathingden]
    #21229010 - 02/05/15 01:35 AM (9 years, 11 months ago)

Sounds like you are doing just fine!

If you dont see contamination or smell anything, chances are the spores are simply taking their sweet time.

Only other thing i can think of is if you inoculated towards the middle of the cake? This can hide the small monokaryotic mycellium colony starting.


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If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
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Offlinefearandloathingden
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Re: Germination [Re: Toadstool5]
    #21229028 - 02/05/15 01:42 AM (9 years, 11 months ago)

Quote:

Toadstool5 said:
Sounds like you are doing just fine!

If you dont see contamination or smell anything, chances are the spores are simply taking their sweet time.

Only other thing i can think of is if you inoculated towards the middle of the cake? This can hide the small monokaryotic mycellium colony starting.




I put the tip of the syringe toward the glass and saw the water run down the jar. So I guess Ill just chill, and start up a new batch to occupy my impatient minds needs.

Thanks for helping me ease my mind Toad!

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InvisibleHashish
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Re: Germination [Re: fearandloathingden]
    #21229976 - 02/05/15 10:50 AM (9 years, 11 months ago)

its too early to even worry. put em up and forget about em for 10 days

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Offlinefearandloathingden
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Re: Germination [Re: Hashish]
    #21239411 - 02/07/15 01:11 PM (9 years, 11 months ago)

Okay so I was told not to worry just yet... But I have been doing some thinking and I think I might have not used enough water in my jars, I followed the 2-1-1 recipe for my jars and I followed it exactly. If I recall correctly there should have been enough moister for some water to be visibly drain if i tilted my bowel i mixed the the verm in. I did not get that. I mixed the brf any ways not wanting to stray from the recipe.

Could this be my problem?

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OfflineThespiceman1028
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Re: Germination [Re: Hashish]
    #21239807 - 02/07/15 02:39 PM (9 years, 11 months ago)

I am a relatively new grower and let me tell you when I first started I checked on my jars daily! Sometimes hourly and I'm sure you know what I mean. I just did some jars and it took them 8 days to show very small signs of growth. I had some even take me two weeks to show sign. Even if u put not enough water u are gonna see signs of growth even if u put too much you will see signs. How did u sterilize the needle before innoculating? I found that just a lighter gave me contams ! Upgrade to a torch


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OfflineMushroom_J
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Re: Germination [Re: Thespiceman1028]
    #21240038 - 02/07/15 03:35 PM (9 years, 11 months ago)

Spores can take up to 21 days to germinate. When you actually see mycellium it's probably already about 5 days from germination. Each spore has to pop open, send out it's hyphae and search for a compatible mate... There's over 20,000 "sexes" of spores... so they have to do a bit of searching for their bride to be lol. sometimes you'll see growth 5 days after inoculation. Sometimes it takes 2 weeks. There's no telling. Spore venders make claims about speed and such for sales purposes... just like any company with a product. It's a selling point for people who are uninformed.

Do you have a thermometer? If so what is temp of the room you're jars are in?
70 f-76f is ideal. If it's lower than 70 it goes slow, but they still grow fine. You just ave to be patient which is hard your first few times.


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Offlinefearandloathingden
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Re: Germination [Re: Mushroom_J]
    #21240046 - 02/07/15 03:38 PM (9 years, 11 months ago)

I keep them around 70-80 degrees (that's just what my room temp is normally)

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Invisiblesooperdooper
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Re: Germination [Re: fearandloathingden]
    #21240142 - 02/07/15 04:01 PM (9 years, 11 months ago)

should prolly take out the vendor name in your op dude. It's not relevant to your question and it's against the rules.

As for your question give it time, it can take well over a week.:shrug:


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OfflineMushroom_J
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Re: Germination [Re: fearandloathingden]
    #21240185 - 02/07/15 04:11 PM (9 years, 11 months ago)

Quote:

fearandloathingden said:
I keep them around 70-80 degrees (that's just what my room temp is normally)




that's fine then. just be patient.
Hang out with friends. It'll keep your mind off of things.
Also don't move the jars much at all.
you don't wan to disturb the verm layer.
That's the usual way people wind up with contams.


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InvisibleToadstool5
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Re: Germination [Re: Mushroom_J]
    #21241731 - 02/07/15 10:58 PM (9 years, 11 months ago)

Quote:

Even if u put not enough water u are gonna see signs of growth




I totally agree, if you used the 2-1-1 ratio then you are fine. It could stunt growth some but it shouldn't cease growth.

Spores take a long time and have a large variance in attributes. This is why everyone likes to isolate a good culture and inoculate from liquid culture or agar wedges.


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups

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Offlinefearandloathingden
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Re: Germination [Re: Toadstool5]
    #21241835 - 02/07/15 11:27 PM (9 years, 11 months ago)

All this clears my mind, thanks you guys. I guess I just have read to many things on the 3-5 day germination time.

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OfflineFodo
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Re: Germination [Re: fearandloathingden]
    #21242565 - 02/08/15 08:13 AM (9 years, 11 months ago)

What about light? Are you giving them 12 hours on 12 hours off light, or are they in the dark all of the time?

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Invisiblesooperdooper
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Re: Germination [Re: Fodo]
    #21242596 - 02/08/15 08:26 AM (9 years, 11 months ago)

Quote:

Fodo said:
What about light? Are you giving them 12 hours on 12 hours off light, or are they in the dark all of the time?



jars dont need to be on a light cycle... just put them on a shelf in ambient indirect light and forget about them... During fruiting Cubensis likes BRIGHT direct lighting.
6500k or fluorescent works well


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InvisibleFunnyFungi
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Re: Germination [Re: sooperdooper]
    #21243106 - 02/08/15 10:32 AM (9 years, 11 months ago)

I have some medicinal jars going that are on week 5 and just finishing up.

I shook them before they got to 30%, which was at week 3 after inoculation and the mycelium really started growing the day after. In other words, in 3 weeks i was only at, id say, 15%..?


Patience really is key.


Then again, i use a lot of grain per jar. I almost fill a quart jar full because i don't find it all that hard to shake and brake the grains up. I also only use 1cc of solution.


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OfflineFreeWorldOrder
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Re: Germination [Re: FunnyFungi]
    #21245373 - 02/08/15 07:47 PM (9 years, 11 months ago)

Yep give them some time... I've seen the beginning of mycelium growth in as little as 3-4 days, all the way up to 2 weeks. This hobby requires patience. And when people rush thing it usually doesn't end on a positive note.

Welcome, and good luck!  :mushroom2:


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Offlinefearandloathingden
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Re: Germination [Re: fearandloathingden]
    #21306466 - 02/20/15 11:14 PM (9 years, 10 months ago)

Okay... An update. I got nothing lol. I figure my spores were no good...Unless it has something to do with altitude, and them not being able to grow this high. But I doubt it. so I will wait till the country warms up and I dont have to worry about spores freezing in transit even though I check weather before hand locally but never thought to check the weather along the shipping route.

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OfflineFodo
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Re: Germination [Re: fearandloathingden]
    #21306983 - 02/21/15 06:50 AM (9 years, 10 months ago)

If you want to take all of the guess work and worry out of your grow, start with spore prints and agar. Syringes are at best a crap shoot, even when applied to agar I have had little success.

Growing mushrooms is all about starting with a few spores and letting the mycelium expand and grow without any contamination along the way. When you start with agar you can see any contamination, just transfer the clean white mycelium to a new petri dish until you have nothing but clean uncontaminated dishes.

Once you have this mastered move on to transferring it to a larger growing medium, grains or cakes whatever your preference. Now you can focus on mastering the next step without worry.

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