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OfflineZebraCopter
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How sterile does spawning need to be! I had an accident.
    #21227232 - 02/04/15 06:31 PM (9 years, 11 months ago)

Today I was mixing my spawn in with the rest of my sub to make a tub while I'm isolating down some other guys on agar. While I was breaking up my spawn I accidentally hit the jar a bit too hard, it shattered, and spawn flew EVERYWHERE. I cleaned it all up and separated it all from the rest of my spawn. I ended up mixing it in with some CCV in a separate, smaller container to see if it would still colonize without contams even though i had to sweep it all up off my floor and rug.

I know this isn't a completely sterile procedure anyway, but my gut says floor spawn will probably contam. Does anyone else have any experience using spawn you didn't think was gonna make it? I'll post pics as soon as I see any progress in the tub.

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OfflinetaGyo
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21227323 - 02/04/15 06:48 PM (9 years, 11 months ago)

You can wipe your ass and spawn and be fine.

Anything floating in the air is in your tub.


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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: taGyo]
    #21227530 - 02/04/15 07:26 PM (9 years, 11 months ago)

That's what I was hoping to hear! Thanks Gyo.

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OfflinetaGyo
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21227540 - 02/04/15 07:28 PM (9 years, 11 months ago)

:salute:


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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: taGyo]
    #21281382 - 02/16/15 07:01 AM (9 years, 10 months ago)

So It's been 12 days since I spawned this dirty spawn and this is what it looks like so far.



It smells kind of sour, which makes me think there's a contam but I can't really see anyhthing except some slight yellowing that appeared this morning. The sides of the container aren't nearly as colonized as the top and this hasn't been cased yet. This contains one quart of spawn and CCV.

I spawned a tub with 2 quarts of spawn and probably a little over a brick of Coir with some verm and coffee thrown in on the same day and it's going much faster. I didn't use a liner so I can see that the sides and bottom of the tub are going much faster than the isolated tupperware. The tub has been cased and is expected to be at 100% colonization when I return from work tonight or wake up in the morning.

Does the isolated tub seem okay? Is the sour smell something to worry about? Should I let it grow and see what happens or play it safe and throw it out?

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Offlineurthtown
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21281393 - 02/16/15 07:05 AM (9 years, 10 months ago)

That's 12 days growth??? That looks really fuckin slow to me... and sour smell is always a bad sign in my experience. I'd say it's fighting a contam.

Just because we spawn in the open air doesn't mean you can shit in your mono and expect it to colonize fine. taGyo is right that it's not sterile once you spawn but if you scrape your spawn off the dirty floor expect a much higher contaminate load that if you just have to deal with what settles out of the air. It may not be sterile once you go to bulk, but you still want to limit the contaminate load to give the myc a better chance.


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Offlineshroom_sensai
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Re: How sterile does spawning need to be! I had an accident. [Re: urthtown]
    #21281452 - 02/16/15 07:23 AM (9 years, 10 months ago)

I bet it'll still make it, CVG is very contam resistant ime


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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: urthtown]
    #21281493 - 02/16/15 07:38 AM (9 years, 10 months ago)

Quote:

shroom_sensai said:
I bet it'll still make it, CVG is very contam resistant ime




Well it's CCV, not CVG, adnd I know sub with coffee is more prone to contam than sub with gypsum is. I hope it makes it, but the slow growth doesn't have me too hopeful.


Quote:

urthtown said:
That's 12 days growth??? That looks really fuckin slow to me... and sour smell is always a bad sign in my experience. I'd say it's fighting a contam.




Yeah it's definitely moving at a snail's pace. If you think the top looks slow you should see the sides (I can't get a not potato quality pic with my camera). There's growth on the sides, but not nearly as much as the top. Most of the growth has happened in the past couple days anyway.

Would dropping all the spawn have shocked the mycelium enough to require that many days to recover before growing out more?

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Offlineurthtown
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21281507 - 02/16/15 07:44 AM (9 years, 10 months ago)

Quote:

ZebraCopter said:

Would dropping all the spawn have shocked the mycelium enough to require that many days to recover before growing out more?




Maybe, but more likely it coated them all in contams and they are having to recover/grow/fight all at once. Probably get a flush off it as it gets desperate to reproduce and if it can colonize the rest of the sub but keep an eye out for green meanie - don't wanna accidentally cover your grow space in trich spores :thumbup:


--------------------
Cluster Headache sufferer? Cluster Busting :thumbup:
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"All mushrooms are edible, but some only once."
-- Croatian Proverb


Edited by urthtown (02/16/15 07:45 AM)

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Offlineshroom_sensai
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Re: How sterile does spawning need to be! I had an accident. [Re: urthtown]
    #21281511 - 02/16/15 07:47 AM (9 years, 10 months ago)

To the above poster, thats not my quote lol.

Sorry, I read CVG, not CCV, my bad.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: urthtown]
    #21281518 - 02/16/15 07:52 AM (9 years, 10 months ago)

Thanks for the advice. I have it totally covered and separate from my other tub. If I see any visual sign of contam I'll probably dispose of it if it doesn't look like it's close to fruiting. Keep your fingers crossed! I'll post updates as soon as it does anything worth mentioning.

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InvisibleBurning
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21281537 - 02/16/15 08:04 AM (9 years, 10 months ago)

I recently spawned a monotub and it's been 4 days. Keeping it at 80-82 degree. I peeked in on it and it shows slow growth no contams that I can see. But it has a fermenty smell to it, also mushroom smell. Does that smell always mean contam? I used coir/Hpoo/verm for a sub

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Offlinegremtu
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Re: How sterile does spawning need to be! I had an accident. [Re: Burning]
    #21281564 - 02/16/15 08:15 AM (9 years, 10 months ago)

My monos had a light windex/mushroom smell. Fruited fine with no contams :smile:


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A Tale of Twelve Tubs

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Offlineshroom_sensai
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Re: How sterile does spawning need to be! I had an accident. [Re: Burning]
    #21281572 - 02/16/15 08:17 AM (9 years, 10 months ago)

Quote:

Burning said:
I recently spawned a monotub and it's been 4 days. Keeping it at 80-82 degree. I peeked in on it and it shows slow growth no contams that I can see. But it has a fermenty smell to it, also mushroom smell. Does that smell always mean contam? I used coir/Hpoo/verm for a sub




I'd keep the temp down in the mid 70s personally, you're asking for contams at 82 imo


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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InvisibleBurning
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Re: How sterile does spawning need to be! I had an accident. [Re: shroom_sensai]
    #21281629 - 02/16/15 08:35 AM (9 years, 10 months ago)

I've read 10 days until I add casing and put tub into fruiting. Do I continue to wait if my sub isn't fully colonized? Also I lowered temp to around 76 degrees, thanks for advice! This is my first go at it, and I did 27 wbs jars over the weekend, I'll start a new log for those when they start to colonize.

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InvisibleFantastic Mr. Fox
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Registered: 02/12/15
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Re: How sterile does spawning need to be! I had an accident. [Re: Burning]
    #21281648 - 02/16/15 08:43 AM (9 years, 10 months ago)

No.. Wait until its 100%, or atleast 100% visibly. :thumbup:


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OfflineFreeWorldOrder
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Re: How sterile does spawning need to be! I had an accident. [Re: Fantastic Mr. Fox]
    #21281744 - 02/16/15 09:13 AM (9 years, 10 months ago)

Right... there is no "set" time for colonization speed. Many factors involved. You want to wait until the surface is all covered in mycelium. If it's cased, I wait until the casing is about 20-30% colonized.


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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: FreeWorldOrder]
    #21312611 - 02/22/15 11:38 AM (9 years, 10 months ago)

It doesn't look like it's gonna make it. I don't see any signs of trich I don't think, but there's been almost no growth since my last update and it continues to smell downright awful. I took it outside to open it and almost threw up when the smell hit me. Oh well!

My other tub has just begun fruiting though and I've counted about 60 pins! Super excited for the first flush to finish off.

I also had some questions about some agar work I was doing. Is there a general rule of thumb to tell if I have a successful isolate by the way it looks? I've already done about 4 or 5 transfers. Also let me know if I should be making a separate thread for this.

This is from the top.



This is from the bottom.


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Offlinemushpunx
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21312722 - 02/22/15 12:03 PM (9 years, 10 months ago)

An isolate will grow out perfectly round. If you put something circular underneath you will better be able to see any sectoring outside the circle (neat trick I learned recently on here).

Its d oubtfull you have an isolate in 4 to 5 transfers if you started from spore.
It usually takes 8 or 9 transfers before I even see sectoring beginning.

You dont really want just one isolate either from a plate from spores.
Once you can distinguish sectors, you want to take each sector and put each one on its own plate. Then you will have to carefully label and fruit out each isolate and keep the really good ones for your collection.

But I say your culture looks pretty clean, so use it as clean innoculant. Grow it out and put it to grain. Do some grows from it and take good clones.

You can fruit out your clones and test em. Again save the good ones!

Then you can go through the process, starting from clone to search fir isolates. At least from a clone you have a better chance of isolating good fruiting strains


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OfflineTrIpPyDuDe
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Re: How sterile does spawning need to be! I had an accident. [Re: mushpunx]
    #21312753 - 02/22/15 12:10 PM (9 years, 10 months ago)

How white should my substrate be before casing?


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OfflinecronicrMFacebook
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Re: How sterile does spawning need to be! I had an accident. [Re: TrIpPyDuDe]
    #21313100 - 02/22/15 01:37 PM (9 years, 10 months ago)

completely


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OfflineTrIpPyDuDe
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Re: How sterile does spawning need to be! I had an accident. [Re: cronicr]
    #21313270 - 02/22/15 01:53 PM (9 years, 10 months ago)

Quote:

cronicr said:
completely



Alright thanks


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OfflinecronicrMFacebook
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Re: How sterile does spawning need to be! I had an accident. [Re: TrIpPyDuDe]
    #21313272 - 02/22/15 01:54 PM (9 years, 10 months ago)

no problem at all and good luck:super:


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OfflineZebraCopter
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Re: How sterile does spawning need to be! I had an accident. [Re: mushpunx]
    #21316417 - 02/23/15 08:22 AM (9 years, 10 months ago)

Okay thanks for the advice. I'll probably do one more isolation and then grow it out on some grains and see if it fruits nicely! I also have a tub going currently that I grew out from one of the earlier plates just so I had something else to do while I was isolating down. Would it still be beneficial to clone a really nice fruit out of this tub?

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OfflinecronicrMFacebook
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Re: How sterile does spawning need to be! I had an accident. [Re: ZebraCopter]
    #21316879 - 02/23/15 10:21 AM (9 years, 10 months ago)

i don't see why not!


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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