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Invisibleblacksabbathrulz
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Registered: 05/22/02
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Please help with buffers
    #2122543 - 11/19/03 01:26 PM (14 years, 1 month ago)

Okay Its been several years since Ive taken gen chem and need to make up buffers for ph3,5,7,9 having a concentration of about 100 millimolar and having similar ionic strengths. THese need to be common lab chemicals as well. IF anyone has any suggestions I would be greatly indebted to them.


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OfflineSeussA
Error: divide byzero

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Re: Please help with buffers [Re: blacksabbathrulz]
    #2122572 - 11/19/03 01:35 PM (14 years, 1 month ago)

You can buy buffer solutions at any chem supply store.


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Invisibleblacksabbathrulz
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Re: Please help with buffers [Re: Seuss]
    #2122582 - 11/19/03 01:38 PM (14 years, 1 month ago)

I dont have time to buy them, I need to make them on friday for an experiment which does not give me much time.


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InvisibleTinMan
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Re: Please help with buffers [Re: blacksabbathrulz]
    #2124040 - 11/20/03 12:12 AM (14 years, 1 month ago)

A buffer solution can be one of the following:

weak acid / conjugate acid
weak base / conjugate base

You could use acetic acid as a weak base, then use the conjugate base (just acetate), pretty much you could use any weak acid for another buffer, but it has to react reversibly with water and form H3O+ ions.


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Invisibleblacksabbathrulz
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Re: Please help with buffers [Re: TinMan]
    #2124568 - 11/20/03 10:02 AM (14 years, 1 month ago)

I figured everything out.
I ended up making the buffers with:
For pH 7
Piperazine-N,N'-bis-(2-ethanesulphonic acid)
(N-(Carbamoylmethyl)-2-aminoethanesulfonic acid
(3-(N-Morpholino)propanesulfonic acid)
Bis-Tris Base
2-[(2-Hydroxy-1,1-bis[hydroxymethyl]ethyl)amino]ethanesulfonic acid

pH 3
Chloroacetic Acid
Formic Acid
Maleic Acid

ph5
Acetic Acid
Propionic Acid
Pyridine
Piperazine

pH 9
Tricine Free Acid
Bicine Free Acid
2-Hydroxy-1,1-bis[bydroxymethyl]ethyl)amino]-1-propanesulfonic acid)
Ethanolamine

and used NaCl for most of the solutions, for a couple I used sodium sulfate.


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