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InvisibleGhatti
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Few questions about LI
    #21204515 - 01/30/15 09:14 PM (9 years, 3 months ago)

So I've done my first LI innoc and all is well although I think either I used too much water or one plate wasn't enough as I didn't feel my Li was "chunky" enough. What's the ratio everyone has been using?

Also I'm worried that I may be pouring too much in and leaving my bags too wet for bacteria.

Any tips tricks and advice for LI would be greatly appreciated.

FYI I put a whole agar plate in 1/2-3/4 pint of water for my Li.

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InvisiblePastywhyteMDiscord
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Re: Few questions about LI [Re: Ghatti]
    #21204869 - 01/30/15 10:46 PM (9 years, 3 months ago)

The thicker LI will recover faster IME. I kinda prefer it a little chunky too, those seem to leap off faster and harder. But your still fine, might take an extra day or two to see recovery but it will still recover.

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InvisibleGhatti
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Re: Few questions about LI [Re: Pastywhyte]
    #21209836 - 02/01/15 06:28 AM (9 years, 3 months ago)

So am I supposed to be using all my LI in one sitting and/or throwing it out or can it safely just be stored in the jar with blender assembly, gasket, and jar ring?

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OfflinetaGyo
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Re: Few questions about LI [Re: Ghatti]
    #21210022 - 02/01/15 07:58 AM (9 years, 3 months ago)

If you have a syringe I'd make a syringe out of it.

Idk about storing it though, that's a good question.


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InvisiblePastywhyteMDiscord
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Re: Few questions about LI [Re: taGyo]
    #21210024 - 02/01/15 07:59 AM (9 years, 3 months ago)

I would be sketch about storing it but I don't like keeping liquid suspended cultures around any longer than I need to.

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OfflinetaGyo
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Re: Few questions about LI [Re: Pastywhyte]
    #21210028 - 02/01/15 08:00 AM (9 years, 3 months ago)

I think Muda and Inoc were saying they only kept LIs around for a month before tossing. Also if you plan on long-term storage I'd use distilled water instead of tap.


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InvisibleGhatti
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Re: Few questions about LI [Re: taGyo]
    #21210119 - 02/01/15 08:39 AM (9 years, 3 months ago)

Eh it doesn't take but a few minutes to make a new one so I'll just make make small chunky batches when need be. What's everyone's recovery/jump off time like?  My first few innocs so far have gone quite slow.

Very whispy growth too just like using an LC. Takes a min to recover but does so all at once.

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OfflinetaGyo
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Re: Few questions about LI [Re: Ghatti]
    #21210127 - 02/01/15 08:43 AM (9 years, 3 months ago)

I got some PESA to recover in 2 days. The trick is blending so it's small enough to be spread out but large enough that you haven't damaged it too much.


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InvisibleGhatti
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Re: Few questions about LI [Re: taGyo]
    #21210252 - 02/01/15 09:26 AM (9 years, 3 months ago)

How long are you pulsing/blending for?

I got recovery kinda after 2-3 days where I could see this whispy myc but it took a while after that to ramp up any.

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