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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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OfflineRohypnol
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Seperating vigerous mycellium on agar?
    #2119915 - 11/18/03 01:17 PM (20 years, 4 months ago)

I have very slow growing mycellium on agar. Its probably growing so slowly because instead of spreading the spores on the agar, I just put them all in one spot. I was wondering if I should find the healthiest mycelilum and use it to start some more dishes. How does one determine which mycellium looks healthier?

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Invisibleblink
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Re: Seperating vigerous mycellium on agar? [Re: Rohypnol]
    #2119926 - 11/18/03 01:20 PM (20 years, 4 months ago)

spread the spores properly, I am certain the FAQ has a picture of how to drag the innoc loop


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Invisiblemicro
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Re: Seperating vigerous mycellium on agar? [Re: blink]
    #2119935 - 11/18/03 01:26 PM (20 years, 4 months ago)

You want fast-growing ropey-looking growth. If you're doing petries you should do more than one, and spread the spores across the dish with a loop or sterilized paperclip w/ small loop at the end.

You want multiple germination sites so you get different colonies so you can select for the best ones.

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OfflineRohypnol
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Re: Seperating vigerous mycellium on agar? [Re: micro]
    #2119956 - 11/18/03 01:31 PM (20 years, 4 months ago)

Should I transfer this mycellium to a few new petri dishes and do it so that I make sure there are multiple germination sites, or should I start from spores again? If I do mycellium, then I can use peroxidated agar, and I don't have a glovebox accessable right now. Is it easy to make multiple germination sites with mycellium?

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Invisiblemicro
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Re: Seperating vigerous mycellium on agar? [Re: Rohypnol]
    #2120015 - 11/18/03 01:47 PM (20 years, 4 months ago)

I'd start from spores again and do more than one dish, this time. If you want to clone mycelium, that's fine, too.

I thought you were trying to select substrains.

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Offlinethisone
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Re: Seperating vigerous mycellium on agar? [Re: micro]
    #2120346 - 11/18/03 03:46 PM (20 years, 4 months ago)

how quickly will the cloned tissue cover the entire agar dish. Recently started agar and too gills in one dish and inner stem in the other. It's been about three days and they have made it through the recovery period (h202 was used) and the mycelium seems to be growing out of the gills that were scattered all over the dish but the stem tissue is not doing much. What is average time, and is it better to take a certain part of the fruitbody or is sterility of the tissue all that matters?

If this isn't advanced enough, my apologies and could this be moved?


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Offlinezeronio
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Re: Seperating vigerous mycellium on agar? [Re: thisone]
    #2124244 - 11/20/03 12:55 AM (20 years, 4 months ago)

I had best results in cloning cubensis when I took the tissue from the stem. It's normal that it takes few days before you notice growth and as soon as you do, transfer the healthy looking mycellium to new dishes. First petri very likely contains contaminants and it takes 2 or 3 transfers before you can assume that you have a clean culture.
Cubensis is pretty fast, a petri is usually fully colonized in 7-10 days.

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Offlinefungulus
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Re: Seperating vigerous mycellium on agar? [Re: zeronio]
    #2128759 - 11/22/03 04:28 AM (20 years, 4 months ago)

Start new ones and then sector the one you have now. Take the best and most vigorous sections and start them in new dishes. This is how you isolate desired fruiting characteristics from multiple (billions) of spores. This is the reason for the transfer. Fruit them out and keep samples of the culture in the fridge. The best fruits win and that culture is used again. Isn't it scientific, dont you think?

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