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Offlinehallucyjunkie
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nothing happened....should i make another jar?
    #21189461 - 01/27/15 11:33 PM (9 years, 3 months ago)

ok so i made one grain jar with a poly fil filter ,using wheat. I injected and have waited 3 weeks .... nothing.

I am now trying two Lcs to inoculate wheat berries.
Should I make another wheat jar with spore syringe and continue lcs ?

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Offliner00tuuu123
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie] * 1
    #21189823 - 01/28/15 02:45 AM (9 years, 3 months ago)

You only made 1 jar? :confused:


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Invisiblehamloaf
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21189911 - 01/28/15 04:23 AM (9 years, 3 months ago)

Quote:

hallucyjunkie said:
ok so i made one grain jar with a poly fil filter ,using wheat. I injected and have waited 3 weeks .... nothing.

I am now trying two Lcs to inoculate wheat berries.
Should I make another wheat jar with spore syringe and continue lcs ?



Those spores should be inoculated to agar instead of the aforementioned mediums.  Grains spawning, and liquid culturing techniques are designed to expand mushroom mycelium, NOT to be used as an inoculation media.

You should forget LC's for now, and research with agar as your main culturing media.  Move on to liquid culturing techniques AFTER you've mastered agar.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinehallucyjunkie
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Re: nothing happened....should i make another jar? [Re: hamloaf]
    #21190771 - 01/28/15 10:42 AM (9 years, 3 months ago)

really? damn i thought you could inc wheat berry jars :mad2: i wasted ... i cant get agar but want to work with grain what can i do i have about 4ccs left.


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Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm :crazy2:                          embolment fluid(once),hash,brownies,  wax,diphenhydramine. :syringe::mushroom2::rasta::tongue:

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OfflineYerow
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21190805 - 01/28/15 10:53 AM (9 years, 3 months ago)

Get some rye berries, WBS, popcorn, whatever. :thumbup:

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Offlinehallucyjunkie
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Re: nothing happened....should i make another jar? [Re: Yerow]
    #21190817 - 01/28/15 10:55 AM (9 years, 3 months ago)

and i can use spore syringe to incubate because my last ones failed...


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InvisibleDrRedBush
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21190825 - 01/28/15 10:59 AM (9 years, 3 months ago)

BRF PF tek works to

Best way to learn IMO

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Offlinehallucyjunkie
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Re: nothing happened....should i make another jar? [Re: DrRedBush]
    #21190864 - 01/28/15 11:07 AM (9 years, 3 months ago)

ok i did sum reading and saw u can use the pf tek to colinize a grain jar is this correct and how do you do it?


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Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm :crazy2:                          embolment fluid(once),hash,brownies,  wax,diphenhydramine. :syringe::mushroom2::rasta::tongue:

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InvisibleFungus Mountain
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21190887 - 01/28/15 11:12 AM (9 years, 3 months ago)

Quote:

hallucyjunkie said:
ok i did sum reading and saw u can use the pf tek to colinize a grain jar is this correct and how do you do it?




Yup, read this.

P2G


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Offlinehallucyjunkie
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Re: nothing happened....should i make another jar? [Re: Fungus Mountain]
    #21191085 - 01/28/15 11:45 AM (9 years, 3 months ago)

ok i will try this does anyone know where to get verm localy .... i made a lc 2 days ago is it ok.


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Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm :crazy2:                          embolment fluid(once),hash,brownies,  wax,diphenhydramine. :syringe::mushroom2::rasta::tongue:

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Offlinehallucyjunkie
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Registered: 11/30/14
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21191108 - 01/28/15 11:50 AM (9 years, 3 months ago)
Log in to view attachment

no cloud


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Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm :crazy2:                          embolment fluid(once),hash,brownies,  wax,diphenhydramine. :syringe::mushroom2::rasta::tongue:

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Offlinehallucyjunkie
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Registered: 11/30/14
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Last seen: 9 years, 3 months
Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21191320 - 01/28/15 12:29 PM (9 years, 3 months ago)

please help im lost ... i want to try another grain jar from a syringe will this work or should i actually buy pf stuff ... or agar?


--------------------
Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm :crazy2:                          embolment fluid(once),hash,brownies,  wax,diphenhydramine. :syringe::mushroom2::rasta::tongue:

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OfflinetaGyo
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21191418 - 01/28/15 12:44 PM (9 years, 3 months ago)

Buy stuff and start PF tek.

http://www.mushroomvideos.com/BRF-Pf-Tek

Do grains after. Knock up 9 jars with 1cc each and keep an extra cc of your syringe.

In this hobby always make more then you need.


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Edited by taGyo (01/28/15 12:45 PM)

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Invisiblehamloaf
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
    #21195984 - 01/29/15 11:08 AM (9 years, 3 months ago)

Quote:

hallucyjunkie said:
really? damn i thought you could inc wheat berry jars :mad2: i wasted ... i cant get agar but want to work with grain what can i do i have about 4ccs left.



You technically, can inoculate grains with spores.  It's just not the best idea due to the time it takes for the spores to germinate.  The time that it takes for spores to germinate costs you about 1-2 weeks of the head start created for the mycelium to run through the grains un contended with, by the sterilization cycle.  That 1-2 week germination time also opens a vector for your hard-worked for grain moisture content to go wary. 

Spores from spore solution in a syringe are best inoculated to agar by dispensing a single drop of the spore solution DIRECTLY in the middle of the media.  Once you find a clean culture or have cleaned a culture up, inoculate said known, clean culture to grains using the "wedge and transfer" technique.  You can then from there spawn the fully colonized grains to bulk substrate materials in preparation for fruiting, or you can exponetially expand your mycelium by thousands using a technique called grain to grain transferring.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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