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Offliner00tuuu123
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Pan Cyan jars not doing any thing
    #21143248 - 01/19/15 10:13 AM (9 years, 3 months ago)

As the title suggests I started 8 Quarts 4 wbs and 4 manure and verm. The wbs was prepared the standard way rinse, soak then boiled drained and pced for 90 minutes at 15 psi. The manure and verm were brought to field capacity then also pced @15 psi for 90 minutes. It's been 2 weeks since inoculation and all the jars look exactly like when they came out of the pc no growth and no contams. The syringe came from a sponsor who I have used many times and the spores could be seen and after a good shake broke up and the water in the syringe I used about 2/3rds to 3/4 cc per jar I'm just confused why not one of the jars has done anything. Any suggestions? :shrug:


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OfflinePinsetter
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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21143297 - 01/19/15 10:26 AM (9 years, 3 months ago)

Two weeks is not long, I have had spores take more than six before any sign. I'd wait at least another two before being concerned.

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Invisiblemycomattie
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Re: Pan Cyan jars not doing any thing [Re: Pinsetter]
    #21144036 - 01/19/15 01:04 PM (9 years, 3 months ago)

Two weeks IS a bit long for Pans.  Also, you should've inoculated just WBS with the syringe; what's the deal with the manure/verm jars?  That mix should only be pasteurized and used as a bulk substrate, not as spawn/inoculant.

Did you inject the 2/3rds - 3/4 cc's along the glass?


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Re: Pan Cyan jars not doing any thing [Re: mycomattie]
    #21144045 - 01/19/15 01:06 PM (9 years, 3 months ago)

don't sterilize manure,did you knock up grains or mix it all?


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Offliner00tuuu123
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Re: Pan Cyan jars not doing any thing [Re: mycomattie]
    #21144372 - 01/19/15 02:11 PM (9 years, 3 months ago)

Quote:

mycomattie said:
Two weeks IS a bit long for Pans.  Also, you should've inoculated just WBS with the syringe; what's the deal with the manure/verm jars?  That mix should only be pasteurized and used as a bulk substrate, not as spawn/inoculant.

Did you inject the 2/3rds - 3/4 cc's along the glass?




The verm and manure mix was just on a whim since I was out of wbs. And yes I noced the jars either along the glass or close to it I put my inoculation ports on one side of the lid and the tyvek breather directly opposite. What mainly puzzles me is there is no growth at all either myc or contams of any kind. :shrug:


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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21144444 - 01/19/15 02:25 PM (9 years, 3 months ago)

Get a pic


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Offliner00tuuu123
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Re: Pan Cyan jars not doing any thing [Re: cronicr]
    #21145507 - 01/19/15 05:52 PM (9 years, 3 months ago)

This is the best pic I could get had to use the webcam on the laptop. But it is pretty clear that I have a jar of a bunch of damp seeds. :shrug: BTW all the jars look like this.


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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21145568 - 01/19/15 06:04 PM (9 years, 3 months ago)

Tell the vendor the spores were not viable under the scope and try again


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Offliner00tuuu123
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Re: Pan Cyan jars not doing any thing [Re: cronicr]
    #21145628 - 01/19/15 06:17 PM (9 years, 3 months ago)

Quote:

cronicr said:
Tell the vendor the spores were not viable under the scope and try again



I still have about 3.5 to 4 ccs of spores left I think I'ma gonna do 3 more jars before I do that, got wbs soaking now. This is my first pan grow but I have a bunch of cube grows under my belt the last and only time this ever happened to me was with some P.E. about 5 years ago. On a side note did that jar look normal to you?


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Invisiblemycomattie
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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21145765 - 01/19/15 06:42 PM (9 years, 3 months ago)

I agree - maybe the spore syringe was not viable/sterile?  I've never used WBS, but the jar looks good :justdontknow:


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Re: Pan Cyan jars not doing any thing [Re: mycomattie]
    #21172223 - 01/24/15 07:06 PM (9 years, 3 months ago)

It's been about 3 weeks and still nothing. I went ahead and used the rest of the syringe on 3 more WBS jars 4 days ago and did 2 boil water LCs as well one WBS jar is showing a small sign of something But it may just be a few burst grains clumped together. So I went ahead and ordered a new syringe but chose Jamaican instead of Austrailian this time Even if only that one jar colonises I will just do G2Gs with it. As of now I'm only down some grain and some time so it is not a total loss and even after 3 weeks none of the jars have contamed So that shows me as far as grain prep everything was done right.  :cookiemonster: 


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Edited by r00tuuu123 (01/24/15 07:10 PM)

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Offliner00tuuu123
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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21190731 - 01/28/15 10:30 AM (9 years, 3 months ago)

Well I finaly gave up on this one and the birds got some food. Thing is when I opened the jars to dump them they were still 'fresh' no contams after 4 weeks. Musta been bad spores. :shrug:


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Re: Pan Cyan jars not doing any thing [Re: r00tuuu123]
    #21190759 - 01/28/15 10:38 AM (9 years, 3 months ago)

look at the bottom do you see a brownish sludge attached to your grain?


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