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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
#21188879 - 01/27/15 09:18 PM (9 years, 3 months ago) |
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Here is my wheat water LC. Came out pretty thick even after a 1:1.5 dilution. Pretty hard to see right now but the myc is going apeshit in there. At this rate I expect it to clear up in about a week to ten days and should be thick with healthy myc 
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hamloaf
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Registered: 12/23/09
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21188913 - 01/27/15 09:24 PM (9 years, 3 months ago) |
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Nice, Pasty. What size jars are those, and how did you inoculate the media.
You wrapped the SHIT out of those the lid seals with the parafilm, didn't you.
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: Goldberg]
#21188919 - 01/27/15 09:25 PM (9 years, 3 months ago) |
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Quote:
Goldberg said: Check the LC!
 Used 3 full syringes just now
Wow. That looks nice. What did you use those three syringes you mentioned using on?
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Pastywhyte
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Registered: 09/15/12
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Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21188931 - 01/27/15 09:27 PM (9 years, 3 months ago) |
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Quote:
hamloaf said: Nice, Pasty. What size jars are those, and how did you inoculate the media.
You wrapped the SHIT out of those the lid seals with the parafilm, didn't you. 
Its a quart jar and it was inoculated with an agar wedge. I had a pin pop up on a plate and I transferred for a clean clone. Then when that grew out I inoculated this LC and a slant 
And yes I did wrap the shit outta it with parafilm. What I lack in equipment I make up for in good choices and paranoia
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21188968 - 01/27/15 09:36 PM (9 years, 3 months ago) |
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Hell yeah, man. Just finished doing something similar two nights ago. 4 monocultures were transferred onto 2 master culture slants per monoculture, then into a quart jar of freshly sterilized WBS.
Best 2 looking monocultures were transferred onto 2 slants, a jar, AND were selected to inoculate those diluted WBS GW liquid mediums featured a few posts back. One media was in a quart jar, and the other was in wide mouth half pint. Both mediums have been fortified with a magnetic stirbar. Can't wait to mix these cultures up with the aid of these stirbars and stirplate.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,866
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21189131 - 01/27/15 10:08 PM (9 years, 3 months ago) |
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One day I will have a doap lab and good equipment. But until then my ghetto methods will serve and force me to be innovative. Working on some crazy shit right now actually
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21189856 - 01/28/15 03:23 AM (9 years, 3 months ago) |
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What are you taking about? Having a "doap lab" will force you to be innovative as well. In fact, you have to achieve a heightened level of intuition into this hobby, and what you're doing in order to even BEGIN to grasp being able to build a lab like I have. Rome wasn't built in a day you know.
Been at this hobby for 5 years now, and have with in the last year FINALLY gotten my techniques and procedures down pact with the equipment, and experience had my disposal. I started in the kitchen with 3 presto23 pressure cookers and a SAB doing LC's with spores using pasteurized wheat straw as the bulk substrate material of choice pasteurized using the pillow case method, and vermiculite was used as a casing layer inside of 18 gallon, gray plastic storage totes, no modifications.
Will grant you that I am very lucky to have a mind with the wherewithall to grasp the new set of skill-sets it takes to even begin to understand how to cultivate mushrooms. That, and I am VERY lucky in the way of finding cultivation related tools and equipment for the BEST prices.
Since I have 5 years of solid experience doing cultivation, I plan on expanding upon that. You don't just throw away ANYTHING you have five years of experience in. The next MAJOR step for me in this cultivation game is to move out of the state I am currently in and into a bigger real estate in another state, go to school for Mycology, and start a legitimate Oyster Mushroom farm on an acre or two with several Mushroom Growing structures, and high-tech, modern, state of the art, research lab, and take it from there. Onward and upward!
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Pastywhyte
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Registered: 09/15/12
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21189988 - 01/28/15 05:14 AM (9 years, 3 months ago) |
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Whoa man, ya got me all wrong. Wasn't trying to imply what you think I was. In fact quite the opposite. Innovation never sleeps in this hobby. But for the low level hobbiest like myself, many of my efforts are merely directed at working around the fact that I do not have access to many of the tools I wish I had access to in order to direct my experiments more efficiently. Certainly your setup is superior to mine however I am certain you have a wish list as well 
In light of my situation many of my innovations are directly related to coming up with methods and ideas that are simply workarounds of my equipment and materials. This is beneficial of course on the level of the average hobbiest but I do look forward to the day when many of my wants are fullfilled
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21190003 - 01/28/15 05:25 AM (9 years, 3 months ago) |
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This is gonna sound strange but using boiled beer could be an option, probably been tried before but beer pretty much contains the same thing as mea -the agar. A sip for me a sip for the agar.
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: r00tuuu123]
#21190016 - 01/28/15 05:32 AM (9 years, 3 months ago) |
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Sounds legit if boiling it removes the carbonation. Don't know how much an issue the carbonation would be, carbonation is CO2, so. Also, don't see the point in boiling the beer before sterilization since no ingredients need to be dissolved into the beer solution. Waste of time, and energy. Try it diluted, and, undiluted, then report the results.
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r00tuuu123
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21190026 - 01/28/15 05:36 AM (9 years, 3 months ago) |
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Quote:
hamloaf said: Sounds legit if boiling it removes the carbonation. Don't know how much an issue the carbonation would be, carbonation is CO2, so. Also, don't see the point in boiling the beer before sterilization since no ingredients need to be dissolved into the beer solution. Waste of time, and energy. Try it diluted, and, undiluted, then report the results.
No boiling to get rid of the alcohol. But then again PCing should do that.
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r00tuuu123
Now I'm just really piseed



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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21190038 - 01/28/15 05:46 AM (9 years, 3 months ago) |
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Quote:
Pastywhyte said: Whoa man, ya got me all wrong. Wasn't trying to imply what you think I was. In fact quite the opposite. Innovation never sleeps in this hobby. But for the low level hobbiest like myself, many of my efforts are merely directed at working around the fact that I do not have access to many of the tools I wish I had access to in order to direct my experiments more efficiently. Certainly your setup is superior to mine however I am certain you have a wish list as well 
In light of my situation many of my innovations are directly related to coming up with methods and ideas that are simply workarounds of my equipment and materials. This is beneficial of course on the level of the average hobbiest but I do look forward to the day when many of my wants are fullfilled 
Pasty you need one of these.
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Please report me to a Mod for hurting your punk ass hippie feelings And all time Champion thread killer.
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hamloaf
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Registered: 12/23/09
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21190042 - 01/28/15 05:50 AM (9 years, 3 months ago) |
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Quote:
Pastywhyte said: I am certain you have a wish list as well 

I want a stainless steel liquid inoculation gun, larger real estate, a better spawn lab made with higher grade sterile materials and decontamination chambers, and Electrostatic air Purifiers from fungiPerfecti for Christmas. Would also like to upgrade the flowhood from one 2x2 foot flowhood into 2 2x4 foot flowhoods of sterile work area on a stainless steel bench.
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21202319 - 01/30/15 01:03 PM (9 years, 3 months ago) |
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Got in late last night from an out of town job to discover that the 1 gallon glass jars with autoclavable closure arrived earlier that day.

Took the frozen, diluted to 50% WBS GW out of freezer to thaw. In the meanwhile, the media vessel closure was modified into a mushroom culture filtration system. Going to be running a liquid culture in one of the gallon sized jars.
Since the liquid media is going to be considerably larger than 1L, increased measures to gas exchange had to be employed <---(Clickey). Instead of going with the standard-average 3 small (5/64) holes in a triangle cluster filter ducting formation, 2, 3/16ths sized holes were drilled into the media vessel closure on opposite sides of each other.





It's not recommended that you use synthetic filter disks as the media filtering material of choice on media vessel closures intended for use with liquid mediums, BUT, it's all that's laying around for right now, PLUS, I have a flowhood, and KNOW how to get away with using synthetic filtering disks as a media filtering material for liquid culturing. Also, now that a magnetic stirplate and magnetic stirbar will be doing the mixing/breaking up of the culture, the vessels containing the live culture inside it's media, and media vessel will NO longer have to be shaken by hand, thus, it's projected through field research study that if the vessel is filled with media to where the jars begin to bend inwards and up to form the top of the jar where the threads are located, the cyclone created by the magnetic stirbar action WON'T get the filtering material wet.
Ordered 2, 2 inch magnetic stirbars, and another one of those circular stirbars three days ago, so I can test which stirbar works best inside of these gallon sized media vessels. Gotta run out to a few towns over to get some grains, then check the Post Office Box.
Moving right along. It's a done deal. Another (well almost another) grow using liquid culturing techniques down, for we have FRUITS from the original end of the boil-cycle grain water featuring rye.




Need to get these tubs into fruiting conditions FAST. They have been is spawn run for 2 days longer than scheduled due to a drop of the hat out of town job. PLUS, the heater malfunctioned while I was out of town, so now the heater has to be replaced, tubs need to have the polyfil pulled, and misted, grow room needs to be brought back up to temp, and proper RH. FUN FUN FUN! We'll see you when all that's done.
This thread has spawned like 10+ other liquid culture experiments. SO much more to come. Thanks for checking this out!
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Pastywhyte
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Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21205979 - 01/31/15 09:10 AM (9 years, 3 months ago) |
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Nice looking fruits man 
My wheat LC inoculated with a unknown clone is really starting to clear up and take off. I figure its gonna be ready in 5 days or so 
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taGyo
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Registered: 10/16/14
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21206171 - 01/31/15 10:03 AM (9 years, 3 months ago) |
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Unknown he says...
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#21206179 - 01/31/15 10:06 AM (9 years, 3 months ago) |
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Indeed it is, I truly have no clue what is in there other than its a cube. I hope it turns out to be PE
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21206346 - 01/31/15 10:51 AM (9 years, 3 months ago) |
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Quote:
Pastywhyte said: Nice looking fruits man 
My wheat LC inoculated with a unknown clone is really starting to clear up and take off. I figure its gonna be ready in 5 days or so 

Looks alright, Pasty. 
I haven't been in my lab since Tuesday, so I don't know what's going on with the recently inoculated WBS mediums with magnetic stirplate and magnetic stirbar fortification. Had to go out of town on business Tuesday afternoon, got back Yesterday, and am still cleaning up, and straightening out my shit. A lab visit is scheduled for tomorrow after I sterilize all these woodchips.
I just boiled 4 different kinds of woodchips for my Pan Stip And Psilo Cyan grows. Apple, pecan, Mesquite, and Cherry, and collected/gathered some undiluted end of the boil-cycle woodchip water. More on that later.
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21207438 - 01/31/15 03:56 PM (9 years, 3 months ago) |
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Quote:
Pastywhyte said:


You know what, good sir, going to have to be honest with you here. Upon further, closer visual examination, that culture, AND the previous picture of the culture in the post above, don't look so good.
Know your camera takes nice pictures yet haven't been able to see through the medium since you've posted pictures of that wheat liquid culture, and, those bubbles (aproxx 4) observed at about the 2 o'clock position in the second picture, and all over the top of the media like that in the first picture are a bad sign. Bubbles that are constantly at the top of the media like that are no-goods.
Bacterial contamination will also RIP though a media as you have described the culture is doing in your post above.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21207479 - 01/31/15 04:07 PM (9 years, 3 months ago) |
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I had just swirled it hard before I took the pic. There are no bubbles now. Regardless I always do a test plate and cake before I knock grains, any bacteria present usually shows up fast on the agar. Here is how it looks now without swirling hard. No bubbles.

I will tread carefully with it tho, I always exercise great care with liquid media. I seen first hand what persistent bubbles usually indicate
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