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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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nothing happened....should i make another jar?
#21189461 - 01/27/15 11:33 PM (9 years, 3 months ago) |
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ok so i made one grain jar with a poly fil filter ,using wheat. I injected and have waited 3 weeks .... nothing.
I am now trying two Lcs to inoculate wheat berries. Should I make another wheat jar with spore syringe and continue lcs ?
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
Posts: 8,507
Loc: I'll be there in a minute
Last seen: 8 years, 2 months
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie] 1
#21189823 - 01/28/15 02:45 AM (9 years, 3 months ago) |
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You only made 1 jar?
--------------------
Please report me to a Mod for hurting your punk ass hippie feelings And all time Champion thread killer.
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21189911 - 01/28/15 04:23 AM (9 years, 3 months ago) |
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Quote:
hallucyjunkie said: ok so i made one grain jar with a poly fil filter ,using wheat. I injected and have waited 3 weeks .... nothing.
I am now trying two Lcs to inoculate wheat berries. Should I make another wheat jar with spore syringe and continue lcs ?
Those spores should be inoculated to agar instead of the aforementioned mediums. Grains spawning, and liquid culturing techniques are designed to expand mushroom mycelium, NOT to be used as an inoculation media.
You should forget LC's for now, and research with agar as your main culturing media. Move on to liquid culturing techniques AFTER you've mastered agar.
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: hamloaf]
#21190771 - 01/28/15 10:42 AM (9 years, 3 months ago) |
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really? damn i thought you could inc wheat berry jars i wasted ... i cant get agar but want to work with grain what can i do i have about 4ccs left.
-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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Yerow
Stranger



Registered: 09/22/14
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21190805 - 01/28/15 10:53 AM (9 years, 3 months ago) |
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Get some rye berries, WBS, popcorn, whatever.
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: Yerow]
#21190817 - 01/28/15 10:55 AM (9 years, 3 months ago) |
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and i can use spore syringe to incubate because my last ones failed...
-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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DrRedBush
I'm unpasteurized


Registered: 12/03/14
Posts: 1,325
Loc: 1600 Pennsylvania Ave NW
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21190825 - 01/28/15 10:59 AM (9 years, 3 months ago) |
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BRF PF tek works to
Best way to learn IMO
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: DrRedBush]
#21190864 - 01/28/15 11:07 AM (9 years, 3 months ago) |
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ok i did sum reading and saw u can use the pf tek to colinize a grain jar is this correct and how do you do it?
-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21190887 - 01/28/15 11:12 AM (9 years, 3 months ago) |
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Quote:
hallucyjunkie said: ok i did sum reading and saw u can use the pf tek to colinize a grain jar is this correct and how do you do it?
Yup, read this.
P2G
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: Fungus Mountain]
#21191085 - 01/28/15 11:45 AM (9 years, 3 months ago) |
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ok i will try this does anyone know where to get verm localy .... i made a lc 2 days ago is it ok.
-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21191108 - 01/28/15 11:50 AM (9 years, 3 months ago) |
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Log in to view attachment
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-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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hallucyjunkie
mycologist


Registered: 11/30/14
Posts: 10
Last seen: 9 years, 3 months
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21191320 - 01/28/15 12:29 PM (9 years, 3 months ago) |
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please help im lost ... i want to try another grain jar from a syringe will this work or should i actually buy pf stuff ... or agar?
-------------------- Mescaline,Lsa,Xtc,n2o,Weed,Shrooms, Dxm embolment fluid(once),hash,brownies, wax,diphenhydramine.   
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21191418 - 01/28/15 12:44 PM (9 years, 3 months ago) |
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Buy stuff and start PF tek.
http://www.mushroomvideos.com/BRF-Pf-Tek
Do grains after. Knock up 9 jars with 1cc each and keep an extra cc of your syringe.
In this hobby always make more then you need.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (01/28/15 12:45 PM)
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: nothing happened....should i make another jar? [Re: hallucyjunkie]
#21195984 - 01/29/15 11:08 AM (9 years, 3 months ago) |
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Quote:
hallucyjunkie said: really? damn i thought you could inc wheat berry jars i wasted ... i cant get agar but want to work with grain what can i do i have about 4ccs left.
You technically, can inoculate grains with spores. It's just not the best idea due to the time it takes for the spores to germinate. The time that it takes for spores to germinate costs you about 1-2 weeks of the head start created for the mycelium to run through the grains un contended with, by the sterilization cycle. That 1-2 week germination time also opens a vector for your hard-worked for grain moisture content to go wary.
Spores from spore solution in a syringe are best inoculated to agar by dispensing a single drop of the spore solution DIRECTLY in the middle of the media. Once you find a clean culture or have cleaned a culture up, inoculate said known, clean culture to grains using the "wedge and transfer" technique. You can then from there spawn the fully colonized grains to bulk substrate materials in preparation for fruiting, or you can exponetially expand your mycelium by thousands using a technique called grain to grain transferring.
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