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OfflineRedRum42
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UV-C used to sanitize?
    #21186858 - 01/27/15 02:43 PM (9 years, 2 days ago)

I see a ton of products on amazon that could potentially be useful. All seem to use UV-C to kill germs/mold/etc claiming 99.9% death.

It seems people hate the whole UV discussion almost as much as buying grow kits here. But i wonder about uses: 1. sterilizing equipment/ grow room before everything 2. sterilizing  substrates- verm, BRF, grain, agar, etc before innoc. 3. sterilizing AFTER noc'd (assuming no, as it would kill spores too?) 4. once a jar/bag is contam'd, UV the shit out of it and the surrounding area, and possibly salvage, or just before throwing away

One product i'm looking at is basically a handheld 'wand' you can hold over things for however long to sanitize. Another product is for toothbrushes and dentures, you place something (conveniently petri-dish sized:cool:)inside a small machine and it closes and sterilizes it.

There are also those 'air purifier' fan/stands. I am wary of these, it seems like bullshit. But there is a reasonably cheap version that uses UV-C to sanitize.

Is it all bullshit? is UV-C not strong enough/ won't kill the right things? Could it even be used after inoculation or would it- i assume- kill mycelium/spores? These things are super cheap, I could easily get all 3 products i described for about $80, and that seems like a small price to pay for a clean room and clean jars without using the inefficient, god forsaken pressure cooker


Edited by RedRum42 (01/27/15 02:57 PM)


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OfflineLittleDaddy
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21186891 - 01/27/15 02:47 PM (9 years, 2 days ago)

I wouldn't completely rely on UV. Autoclave/pressure-heat sterilization is definitely preferred.


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflineX0cyris
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Re: UV-C used to sanitize? [Re: LittleDaddy]
    #21186916 - 01/27/15 02:53 PM (9 years, 2 days ago)

only need uv-c to sterilize polystyrene petri's and i read a post by RR no matter how you slice it it is more cost effective to just buy/dispose as the energy costs to sterilize even glass petri's is more than the dish itself costs.


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The first step to being part of something bigger than yourself is realizing you already are.



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OfflineRedRum42
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Re: UV-C used to sanitize? [Re: X0cyris]
    #21186935 - 01/27/15 02:55 PM (9 years, 2 days ago)

I'm not too worried about cost. The cost of the actual machine/products is surprisingly low, and I don't mind a little extra on the energy bill.

I just want to know if and how it would work in practice. I have a personal vendetta against pressure cookers, long story, so I just wanted to see if I could live without one.

Maybe boiling shit with a combo of U.V. to replace p.c-ing?

thanks a lot for the fast replies. Much appreciated.


Edited by RedRum42 (01/27/15 02:58 PM)


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OfflineX0cyris
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21186972 - 01/27/15 03:02 PM (9 years, 2 days ago)

using both would be silly imo. UV is very effective if not cost effective though. I have a uv sterilizer used for tattoo needles etc but you wouldn't be able to fit more than 1- petri's in it y'know?


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The first step to being part of something bigger than yourself is realizing you already are.



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OfflineJustamaninafish
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21186996 - 01/27/15 03:07 PM (9 years, 2 days ago)

UV requires certain amount of time however the reason i would caution you to use it is that it requires line of sight. AKA if something is under the surface or on the bottom of a jar or utensil it wont get sterilized unless its getting hit by the uv light *duh.
SO its simply surface sterilization. Couldnt sterilize grains or anything.

As others have pointed out its used primarily in labs as a supplement to kill/prevent bacteria.

So sorry your not unchaining yourself from the PC yet :P


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OfflineLittleDaddy
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Re: UV-C used to sanitize? [Re: Justamaninafish]
    #21187100 - 01/27/15 03:22 PM (9 years, 2 days ago)

Quote:

Justamaninafish said:
As others have pointed out its used primarily in labs as a supplement to kill/prevent bacteria.

So sorry your not unchaining yourself from the PC yet :P




Yeah, the biohood I work in has a UV light, but the agar is always sterilized in the autoclave, then poured in the biohood, wrapped, and exported to the fridge. When we import it back in for inoculation, we spray the wrap with ethanol. The UV light is then left on for about 10 minutes. Just another precaution, but I've always been told to never rely on it.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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InvisiblebodhisattaMDiscordReddit
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Re: UV-C used to sanitize? [Re: LittleDaddy]
    #21187142 - 01/27/15 03:31 PM (9 years, 2 days ago)

really a waste of time. there's to parts to the hobby the sterile side and the not sterile side.
there's no sanitary part. so the light is pretty fucking useless.

you sterilize anything you're putting your clean culture into. when you want to fruit it that can be done open air.

trying to "sanitize" a room before doing sterile work is just a way for us to know you don't understand sterile technique and this hobby enough


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OfflineLittleDaddy
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Re: UV-C used to sanitize? [Re: bodhisatta]
    #21187176 - 01/27/15 03:36 PM (9 years, 2 days ago)

Quote:

bodhisatta said:
so the light is pretty fucking useless.

trying to "sanitize" a room before doing sterile work is just a way for us to know you don't understand sterile technique and this hobby enough



Quote:

Learn agar, build stuff, do stuff, don't bully




--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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InvisiblebodhisattaMDiscordReddit
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Re: UV-C used to sanitize? [Re: LittleDaddy]
    #21187228 - 01/27/15 03:47 PM (9 years, 2 days ago)

bullying would be saying
how fucking stupid are you OP no this woudn't fucking work it's usless and clearly shows you don't know what the fuck you're talking about. go back to square one.

but really, there's no need for a uv light. it doesn't integrate into mushroom growing since there's no part of the process where it's more useful than easier, cheaper, better techniques.


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OfflineKizzle
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Re: UV-C used to sanitize? [Re: bodhisatta]
    #21187704 - 01/27/15 05:23 PM (9 years, 2 days ago)

Quote:

only need uv-c to sterilize polystyrene petri's and i read a post by RR no matter how you slice it it is more cost effective to just buy/dispose as the energy costs to sterilize even glass petri's is more than the dish itself costs.



If you were sterilizing them one at a time perhaps but you could fit dozens in an oven and it only costs maybe 10 cents to run it.


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OfflineMushroom_J
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Re: UV-C used to sanitize? [Re: Kizzle]
    #21188028 - 01/27/15 06:24 PM (9 years, 2 days ago)

Off topic lol...

There's a few apple orchards around that make cider.
All except one boil their cider to pasteurize it.

The one exception uses UV to pasteurize.
Their cider tastes the best. Boiling effects the natural sugars.


UV wouldn't do much in this hobby. It's a last precaution.

Proper teks and maybe antibiotic agar is all you need.... sab /flowhood etc


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OfflineLittleDaddy
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Re: UV-C used to sanitize? [Re: Mushroom_J]
    #21188206 - 01/27/15 06:58 PM (9 years, 2 days ago)

there are some pretty cool UV pens for hiking and sterilizing water, too!


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


Edited by LittleDaddy (01/27/15 07:15 PM)


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OfflinePsilicon
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Re: UV-C used to sanitize? [Re: Justamaninafish]
    #21188380 - 01/27/15 07:33 PM (9 years, 2 days ago)

Quote:

Justamaninafish said:
UV requires certain amount of time however the reason i would caution you to use it is that it requires line of sight. AKA if something is under the surface or on the bottom of a jar or utensil it wont get sterilized unless its getting hit by the uv light *duh.
SO its simply surface sterilization. Couldnt sterilize grains or anything.

As others have pointed out its used primarily in labs as a supplement to kill/prevent bacteria.

So sorry your not unchaining yourself from the PC yet :P




:whathesaid:

UV can't penetrate grains.  Sorry, OP.

Gamma rays, though...


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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OfflineRedRum42
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21189115 - 01/27/15 10:05 PM (9 years, 2 days ago)

Some ridiculous shit going on here. It has been all but proven UV-C is great for supplemental use, in addition to standard methods. If I can give 90% cleanliness with usual methods, and UV gives me an extra 5%, fuck its worth it to me.

I never said I expected it to penetrate anything. I know it doesn't even penetrate glass/plastic let alone something opaque

And bodhistta, I may have an elementary understanding of mycology, but you clearly have a tenuous grip on science and basic common sense. Will it make my clean room dirtier? Will it somehow ruin everything? How can you so vehemently hate something as objective as wanting a little extra bang in my cleanliness routines? How does me spending $15 on a shitty UV-C wand as an experiment offend you?

This is a wonderful site with oodles of priceless info and experimentation furthering mycology as a whole, but some a couple pompous folk really spoil it for the rest. Experimentation always leads to the future and superior methods. Failures weed out what doesn't work, and shows you what does. It harms no one except for the experimenter who should be prepared to have wasted their money on something that did work. Ever single tek here would have been ridiculed in the 70s. It is easy to stick to what everyone else says as you take no risk and don't have to fire up any intelligence.


Edited by RedRum42 (01/27/15 10:12 PM)


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OfflinePsilicon
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21189141 - 01/27/15 10:11 PM (9 years, 2 days ago)

Quote:

RedRum42 said:
2. sterilizing  substrates- verm, BRF, grain, agar, etc before innoc.




So you want do what?  Just lay them out in a row and zap them?


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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OfflineRedRum42
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Re: UV-C used to sanitize? [Re: Psilicon]
    #21189152 - 01/27/15 10:13 PM (9 years, 2 days ago)

Mostly going for the empty grow room and tools before use/ jars before full etc. Also possibly cleaning the room after it has been full of contam'd jars.

From my research it seems this should work. obviously will avoid it shining on my skin/eyes, and am aware it degrades paint/plastic/etc etc

Totally worth the small cost if even if i get a small, small percentage less of contam.s


Edited by RedRum42 (01/27/15 10:14 PM)


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OfflinePsilicon
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21189159 - 01/27/15 10:14 PM (9 years, 2 days ago)

It's cool that you've changed your mind between the original post and now, but don't get mad at me because I replied to a thread asking about using UV to sterilize grain and you decided in the meantime you didn't want to do that without telling anybody.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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OfflineRedRum42
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Re: UV-C used to sanitize? [Re: Psilicon]
    #21189167 - 01/27/15 10:16 PM (9 years, 2 days ago)

Sorry man didn't mean for my post to reflect on your replies specifically, your responses were helpful and objective. Thanks again for the quick replies

It is frustrating dealing people like 'bodhista' who seem so offended by a (not even that really) novel way of doing things


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OfflinePsilicon
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Re: UV-C used to sanitize? [Re: RedRum42]
    #21189174 - 01/27/15 10:17 PM (9 years, 2 days ago)

It's easy to misread bodhi's remarks if you don't know him very well.  He really, really likes the word "fuck."  He's here to help and be constructive, I promise.  As far as whole-room sanitization or air sanitization goes, either one could potentially be helpful in reducing trich spore count, but I'm not sure about that at all.  It would be interesting to see.  Wouldn't work as well as a laminar flow hood in cleaning out a room and providing sterile air, but might be an easier workaround.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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