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Tryptamole
Stranger
Registered: 10/16/14
Posts: 6
Last seen: 5 years, 7 months
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Incubation in glovebox?
#21188287 - 01/27/15 07:15 PM (9 years, 3 months ago) |
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I hope this isn't too daft of a question, but would there be any issues with just leaving my PF tek jars inside the glovebox after inoculation, as an incubator of sorts? I'm also getting very mixed info on the dark/light situation for incubation, some say complete darkness, others indirect sunlight, can anybody shed any light on the situation ? Pun almost not intentional. Cheers. Mole.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
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Re: Incubation in glovebox? [Re: Tryptamole]
#21188340 - 01/27/15 07:26 PM (9 years, 3 months ago) |
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Yeah, you can leave them in there. They do not benefit from being left in the dark, and some say they do benefit from the light.
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Tryptamole
Stranger
Registered: 10/16/14
Posts: 6
Last seen: 5 years, 7 months
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Re: Incubation in glovebox? [Re: Psilicon]
#21202719 - 01/30/15 02:18 PM (9 years, 3 months ago) |
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Thanks for the reply! my glovebox is 100% sealed, so as its already sterile, seems perfect. I'm guessing there will be enough air, and GE is more important from within the jar, right?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Incubation in glovebox? [Re: Tryptamole]
#21202725 - 01/30/15 02:19 PM (9 years, 3 months ago) |
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Detch your gloves so it is a still air box
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Incubation in glovebox? [Re: cronicr]
#21202740 - 01/30/15 02:22 PM (9 years, 3 months ago) |
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Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.
Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.
Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.
P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.
Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.
You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.
You want direct/intense 6500K light on a 12/12 schedule for fruiting.
Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.
Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.
The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 83F , and contams thrive.
Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.
Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years. Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger. RR
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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