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Invisiblebudmanman
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Re: My Agar VS. Your Agar.... [Re: blindingleaf]
    #21172273 - 01/24/15 07:25 PM (9 years, 3 months ago)

Quote:

blindingleaf said:
yea, u just used agar w/o nutrients.

if u/ur friend/ur family/ur neighbor has a dog or a cat, use 10g dry kibble food, 10g agar powder, and 500ml water.  thats seriously the easiest recipe i have ever used, and have not had a species or strain yet that didn't like it.  dog and cat food have so many ingredients and so many different kinds of nutrients (fats, proteins, carbs, etc) that it would surprise me if a culture did not respond well to it.

i don't know much about grain water.  i used to use it more, but was mixing it with malt extract.  so not sure on dilutions.  maybe pasty or violet would know better.





I would not use cat food as cats eat mostly meat.


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Invisibleblindingleaf
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Re: My Agar VS. Your Agar.... [Re: budmanman]
    #21172305 - 01/24/15 07:33 PM (9 years, 3 months ago)

TBH, i never used cat food, but have read its fine.
i know only difference btw cat and dog food mostly is taurine.  cats require more than dogs in their diet.

mushpunx, yea grind it up well, put it in hottest water from ur tap, shake, cover, PC.


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InvisibleNumeroEno
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Re: My Agar VS. Your Agar.... [Re: blindingleaf]
    #21172603 - 01/24/15 08:45 PM (9 years, 3 months ago)

I want to know this too. I just bought a pound of GLME and I already have potato starch, dextrose, and nutritional yeast. I've read that rotating your agar media is good so I'd like to get DFA down so I have one more agar recipe to use.


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As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: NumeroEno]
    #21199264 - 01/29/15 10:01 PM (9 years, 3 months ago)

OK. A few slices of "weak" agar have been tranfered to mixed agar with (hopefully) proper nutes. I mixed 3.5g agar with 3.5 potato flakes and a drop of honey in 250ml of water.


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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21216609 - 02/02/15 03:20 PM (9 years, 3 months ago)

IM DOING IT!!!!



After a few failed attempts I am finally seeing agar growth from clone! Thanks for all the support. you guys rock! Couldnt have come this far in the hobby without all the beautiful minds of the shroomery.


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Invisiblebudmanman
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21216643 - 02/02/15 03:28 PM (9 years, 3 months ago)

Did you let your agar cool with a feq pennies under neath its left side there?


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And I am mentally unstable.

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InvisibleMunchauzen
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Re: My Agar VS. Your Agar.... [Re: budmanman]
    #21216690 - 02/02/15 03:38 PM (9 years, 3 months ago)

Quote:

budmanman said:
Did you let your agar cool with a feq pennies under neath its left side there?




he probably left the pc cool down completely before removing them.

I always remove my plates hot, so they can cool on the level countertop.

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InvisibleStipe-n CapMDiscord
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Re: My Agar VS. Your Agar.... [Re: budmanman]
    #21216706 - 02/02/15 03:40 PM (9 years, 3 months ago)

Quote:

budmanman said:
Yeah bro you gotta add some potatoes to that lol.




So are you guys talking about pre-made agar or home made? For instance I use this agar:

http://www.mycosupply.com/culture%20media/

Would I need to make amendments to this?

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OfflinetaGyo
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Re: My Agar VS. Your Agar.... [Re: Munchauzen]
    #21216734 - 02/02/15 03:45 PM (9 years, 3 months ago)

Quote:

Munchauzen said:
Quote:

budmanman said:
Did you let your agar cool with a feq pennies under neath its left side there?




he probably left the pc cool down completely before removing them.

I always remove my plates hot, so they can cool on the level countertop.



I fell asleep once with 8 plates in my PC.

Woke up at 2 am, jumped out of bed, ran to my kitchen, opened the ice fucking cold PC and the top 4 had their agar solidy on the fucking sides.

SO pissed.

Used them anyway.


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InvisibleMunchauzen
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Re: My Agar VS. Your Agar.... [Re: taGyo]
    #21216806 - 02/02/15 03:59 PM (9 years, 3 months ago)

you can just turn the heat back on till the steam starts, should be a liquid again by then

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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: Munchauzen]
    #21216931 - 02/02/15 04:28 PM (9 years, 3 months ago)

I'm not sure what your asking....why would I put pennies in my agar? I let it cool and then transferred the tissue onto the plate in my SAB....am I missing something here?


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OfflinePsilicon
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Re: My Agar VS. Your Agar.... [Re: Stipe-n Cap]
    #21217031 - 02/02/15 04:47 PM (9 years, 3 months ago)

Quote:

p9hu7 said:
Quote:

budmanman said:
Yeah bro you gotta add some potatoes to that lol.




So are you guys talking about pre-made agar or home made? For instance I use this agar:

http://www.mycosupply.com/culture%20media/

Would I need to make amendments to this?




There are two products on that page.  With one you'd have to supplement, but the other is fine as it is.


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
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InvisibleStipe-n CapMDiscord
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Re: My Agar VS. Your Agar.... [Re: Psilicon]
    #21217061 - 02/02/15 04:52 PM (9 years, 3 months ago)

I didn't notice, I have the MEA not the bacterialogical.

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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: Stipe-n Cap]
    #21217070 - 02/02/15 04:54 PM (9 years, 3 months ago)

How is antibacterial agar made? Would it be worth adding a few drops of hydrogen peroxide?

kind of pointless I suppose because if mold appears on agar then we just cut out a good piece and transfer. Thats the whole point!!!!


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Invisiblebudmanman
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21217087 - 02/02/15 04:55 PM (9 years, 3 months ago)



I transferred this to a new plate, the new plate seems like its fine.


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InvisibleStipe-n CapMDiscord
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21217099 - 02/02/15 04:57 PM (9 years, 3 months ago)

Antibacterial is for long term slant storage.

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OfflinePsilicon
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21217100 - 02/02/15 04:57 PM (9 years, 3 months ago)

No, it wouldn't be worthwhile to add peroxide.  For one thing, fungal spores don't produce catalase--just hyphae.  Any spores would be eradicated if you put them to a peroxidated agar, if the agar did manage to stay peroxidated, which is the other potential problem.

There are two ways to do antibacterial agar.  One is to buy gentamycin sulfate, which can survive a PC cycle.  The other is to PC a poured agar, let it cool a bit, and then when it's below the denaturing point but above the gelling point, add the antibiotic, swirl to dissolve, and then pour.


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: budmanman]
    #21217106 - 02/02/15 04:58 PM (9 years, 3 months ago)

nice.


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OfflinePsilocyBen17
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Re: My Agar VS. Your Agar.... [Re: PsilocyBen17]
    #21217109 - 02/02/15 04:58 PM (9 years, 3 months ago)

this has been one of the more valuable posts I've made. great thread. Thanks for the input.


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InvisibleStipe-n CapMDiscord
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Re: My Agar VS. Your Agar.... [Re: Psilicon]
    #21217166 - 02/02/15 05:09 PM (9 years, 3 months ago)

So how many gr of dehydrated potatoe flakes do you need to add to 500 ml of water?

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