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OfflineMycologist217
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Re: Post Your Cultivation Picture of The Day [Re: Bobbio]
    #25621642 - 11/17/18 06:10 PM (5 years, 2 months ago)

Well... generally if the plate itself was dirty then you would have "satellite colonies" but you don't have that here...if that is mold in the center of the plates then it was present when the transfers were made...not many molds have rhizomorphic mycelium so that is probably your best bet for cleaning up the culture via transfer


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisibleGorilla91
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Re: Post Your Cultivation Picture of The Day [Re: Jakeoncid419] * 2
    #25621683 - 11/17/18 06:21 PM (5 years, 2 months ago)



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[gradient:#FF0000,#]We are part of this universe; we are in this universe, but perhaps more important than both of those facts, is that the universe is in us[/gradient]


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Offlinetryptkaloids
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Re: Post Your Cultivation Picture of The Day [Re: Mycologist217] * 1
    #25621694 - 11/17/18 06:26 PM (5 years, 2 months ago)

Quote:

generally if the plate itself was dirty then you would have "satellite colonies"


that is one of many indicators of a dirty plate. but not the most important. the easiest contams to see and get rid of are satellite colonies. but hidden contams are the bane of my existance


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleJHOVA
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Re: Post Your Cultivation Picture of The Day [Re: Mycologist217]
    #25621709 - 11/17/18 06:28 PM (5 years, 2 months ago)

Quote:

Mycologist217 said:
Well... generally if the plate itself was dirty then you would have "satellite colonies" but you don't have that here...if that is mold in the center of the plates then it was present when the transfers were made...not many molds have rhizomorphic mycelium so that is probably your best bet for cleaning up the culture via transfer




mold sucks when it get's wrapped up in mycelium. check out these fucked up plates. i went back to new germ plates after seeing mold win the war.


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OfflineBobbio
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Re: Post Your Cultivation Picture of The Day [Re: JHOVA]
    #25621743 - 11/17/18 06:46 PM (5 years, 2 months ago)

Damn. I can see in JHOVA pictures the almost clear mycelium that is around the edges of the white as well, all my plates have that in a certain light. Maybe the APE swap had some nasties on it from the get go :shrug:

Maybe I'll just transfer once more, and see if these plates pin or even keep going


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"We all live incredibly different lives each with our own tragedy and triumph, and this one, incredible, amazingly border shattering and life altering thing we call mycology has bound us all together. Through a combination of technology, research, and strife we have found a place that unites us like a mycelial network of souls to a science that brings us warmth and comfort. It's a pleasure knowing all of you."

-TravelAgency


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OfflineMycologist217
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Re: Post Your Cultivation Picture of The Day [Re: Bobbio]
    #25621843 - 11/17/18 07:37 PM (5 years, 2 months ago)

The point of agar work is to clean up dirty culture...there should never be a point when you are stuck ... invisible contaminations on agar? I mean it will sporulate eventually no matter what. I know a lot of you guys aren't using flowhoods but a dirty agar plate is never a show stopper...unless you are just outright bad at sterile technique or you just don't understand the fundamentals (the "why's?") of it all



--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineCzM8
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Re: Post Your Cultivation Picture of The Day [Re: Mycologist217]
    #25621851 - 11/17/18 07:44 PM (5 years, 2 months ago)

Are thes contams in the cultures? I have 3 different cultures and they keep transferring like this. I actually finally took a plate to grains and ithose grains are in coir right now.

I swear no matter how I transfer it, the cultures look like this. Maybe I need to go to a SAB instead of a hood?





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OfflineMycologist217
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Re: Post Your Cultivation Picture of The Day [Re: CzM8]
    #25621861 - 11/17/18 07:51 PM (5 years, 2 months ago)

That just looks like sectoring to me. Is this supposed to be an isolate? If not keep in mind as the individual strains seperate the mycelium will look different from strain to strain...we are generally disposed to going for the most rhizomorphic strains as far as cubes go.I dont think that is contamination.it can take many transfers to get down to a monoculture


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisibleJHOVA
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Re: Post Your Cultivation Picture of The Day [Re: CzM8]
    #25621862 - 11/17/18 07:52 PM (5 years, 2 months ago)

where would you take a wedge of the agar i posted myco217? Those are T4’s. Its less work in my opinion to streak a fresh plate and start wth less mess.

If you have some pics post em of bad contams you have gotten away from.


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OfflineMycologist217
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Re: Post Your Cultivation Picture of The Day [Re: JHOVA]
    #25621884 - 11/17/18 08:04 PM (5 years, 2 months ago)

I would transfer from the outermost edge at the 9 o'clock position on your second plate pic

I transferred away from this and cleaned it up:


That being the case...contamination shouldn't be a constant hurdle...it should be something you overcome through learning proper sterile technique which myself and several AMU members have links to instructional videos in their sigs


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineCzM8
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Re: Post Your Cultivation Picture of The Day [Re: Mycologist217]
    #25622025 - 11/17/18 09:23 PM (5 years, 2 months ago)

I wasn’t going for a monoculture, but I wanted to get more uniform growth.


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OfflineelPeligroso 5150
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Re: Post Your Cultivation Picture of The Day [Re: mushboy] * 1
    #25622086 - 11/17/18 10:05 PM (5 years, 2 months ago)

Quote:

mushboy said:
m0r mushcult


putting this one too grains :rockon:



Nice! Gonna kill with PE melmac Cant wait to see those caaks in here! Nice culture.

melmac


1:1.5 spawn/sub ratio 39g dry 1st 2nd 32g  71g total dry


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Offlinemushroomnate
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Re: Post Your Cultivation Picture of The Day [Re: elPeligroso 5150]
    #25622106 - 11/17/18 10:25 PM (5 years, 2 months ago)

Quote:

mr.nelsonmtman said:
melmac


1:1.5 spawn/sub ratio 39g dry 1st 2nd 32g  71g total dry





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BRF to bulk/Cakes as spawn


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OfflineLeon ForestFacebookDiscord
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Re: Post Your Cultivation Picture of The Day [Re: mushroomnate]
    #25622147 - 11/17/18 11:21 PM (5 years, 2 months ago)

stones


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Offlinemushroomnate
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Re: Post Your Cultivation Picture of The Day [Re: Leon Forest]
    #25622153 - 11/17/18 11:26 PM (5 years, 2 months ago)

Some 3rd flush chubs on a neglected sub.


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BRF to bulk/Cakes as spawn


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Post Your Cultivation Picture of The Day [Re: mushroomnate]
    #25622291 - 11/18/18 02:57 AM (5 years, 2 months ago)

Quote:

mushroomnate said:
:awesomenod:
Tammed it perfectly.:lol: :timeismoney:



That's pretty punny!

I try to use the first clean plate, keeping initial MS tubs as genetically variant as possible personally  :shrug:
Clone several from that tub for side by side's in shoeboxes, picking and slanting my favorite for further isolation projects,  based on colonization speed, first flush yeild, and potency... I keep the original clone culture slanted bc I've only had one isolate perform better than the clone it stemmed from


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Offlineshroompy
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Re: Post Your Cultivation Picture of The Day [Re: Nimph]
    #25622398 - 11/18/18 05:56 AM (5 years, 2 months ago)

Quote:

Quirkmeister92 said:
:costanza:
Them is purty ones. Hows potency compared to cubes?




I have no idea. I just like to grow mushrooms ^^

Quote:

Nimph said:
You beat me to it lol, can u give me a quick run down of what u did?/fruiting wise?




Used a Autoclavable microbox, spawn with LC, when it was fully colonized cased with Coir/Verm/Gypsum and went to fruit where other cubis fruit. Big Tote with big holes patched with Tyvek. Mist every other day, and wait... then wait some more.


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Post Your Cultivation Picture of The Day [Re: shroompy] * 2
    #25622775 - 11/18/18 09:58 AM (5 years, 2 months ago)


Made this beast today should easily be able to wash and strain 10-20 pounds of grain


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OfflineJimshorts
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Re: Post Your Cultivation Picture of The Day [Re: shroompy]
    #25622784 - 11/18/18 10:02 AM (5 years, 2 months ago)

Here's what I've got going on...  5 tubs in a GH.  One had the colonized WBS fortified with Brf, the rest all the same otherwise.

...and apparently one is growing upside down.



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Offlinehamonz
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Re: Post Your Cultivation Picture of The Day [Re: Jimshorts]
    #25622799 - 11/18/18 10:07 AM (5 years, 2 months ago)

Quote:

Jimshorts said:
Here's what I've got going on...  5 tubs in a GH.  One had the colonized WBS fortified with Brf, the rest all the same otherwise.

...and apparently one is growing upside down.






Thats awesome!!!


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