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Offlinehoffzl01
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Freedom Fungus - First time grow needs opinions
    #21164481 - 01/22/15 07:30 PM (9 years, 11 months ago)

Hey all - First time cultivator here.  I've been reading your boards for weeks and read most of Stamet's works...ready to try my hand.

So I'm deployed to the sandy and sunny part of our planet, and decided I wanted to try growing some oyster mushrooms here.  I ordered a grow kit instead of spawn or spores, to get my initial sample from.  I've also ordered some basic materials (agar, petri dishes, scalpals, etc).

So I need opinions and suggestions on the following plan.  Anything is appreciated...even if you think there's not a chance in H### this will work..I'd like to know.

Keep in mind...even basic laboratory sterilization is close to impossible, as I live and work in dusty sandy tents.

- Grow Blue Oysters out with a kit (dont see too much of a problem here, pretty straight forward.  get it wet, keep it humid, grow it out)
- harvest fully grown mushrooms...keep good samples...cook and enjoy the rest.
- make spore prints from good fruits / make tissue plates from good fruits
- *** What do you guys/gals think about the feasibility of using cooked ramen broth supplemented with sugar or weak coffee as Agar?  It's about the only consistent carbohydrate source I can get my hands on besides paper products
- *** I have no idea how to do any basic sterilization for the agar medium.  we have to get creative here!  I have a coffee maker and a lighter. :smile:  mushroom McGyver style.
-  I want to practice my technique for isolating strains, but that might have to wait until I get home...as I imagine I would really be practicing my technique for managing stress as my plates get continuously contaminated.
- If I can make successful agar plates, I want to try store bought microwave popcorn as the substrate for grain spawn.  We have more than a small country could ever eat.  I can use empty bottled water containers, and coffee cans as "jars". Ideas?
- The goal is to use all of the boxes that we receive care packages in, as the bulk substrate.  They end up in the trash, and I have a hunch that if I can supplement them in the right way..the shredded boxes might make good mushroom food.  (probably good bacterial food as well, but we'll see)

1)  Any ideas on how to get some basic pasteur/sterilization going with a microwave, a coffee maker, and nothing but plastic bottles?

2)  Will those substrates work?  I'd love to get to the point that we can grill up some delicious mushrooms grown on the overabundance of unused crap we have laying around. 

Thanks!

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InvisibleHelnak
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21164565 - 01/22/15 07:51 PM (9 years, 11 months ago)

I remodeled my kitchen a few years back, and while I was without proper appliances I learned that Styrofoam can be nuked long enough to boil water.  It may be able to last long enough for partial sterilization?  Just a thought.

As for the cardboard, it would probably make a great substrate.  I have never used it solely for a substrate, but I have had culture samples start to pin and fruit from small wedges. 

After your grow kit fruits, take a mushroom that has some visible mycelia at the base of the stem and keep it wrapped up and clean.  Now split a piece of cardboard so the corrugation is visible.  Dampen inside of cardboard, sandwich your clean stipe in the cardboard.  Tuck away somewhere relatively clean and out of dusty winds.  Wait 2-4 days and the mycelia should be spreading up the cardboard.  Use several of these as spawn for bulk.

Edited by Helnak (01/22/15 08:07 PM)

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: Helnak] * 1
    #21164659 - 01/22/15 08:06 PM (9 years, 11 months ago)

Well noted Helnak.  I appreciate it. I do have Styrofoam coffee cups.  I considered just nuking the liquids (with agar in it already) long enough to achieve boil, but I didn't know if a quick boil was enough.  And then pouring while still hot into dishes cleaned with isopropyl alcohol.  Letting it firm up as it cools. I might have to twist a few arms to get some ISA though. It's certainly better than nothing.


--------------------
He's not the sharpest cookie in the cupboard.  What?

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OfflinePsilicon
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21164834 - 01/22/15 08:35 PM (9 years, 11 months ago)

There's a whole lot to reply to here.  I like a challenge, but IMO you're going to need to meet us halfway and procure a pressure cooker somehow.  Consult the dog robber for one of the people in charge.  He'll be able to rustle you up one, I'm sure.

FYI, we also have a gourmet and edibles section, where you can get advice more tailored to the species you're looking to grow, but I think on the whole we're adequate to answer your questions about oysters and agar.

Quote:

hoffzl01 said:
- *** What do you guys/gals think about the feasibility of using cooked ramen broth supplemented with sugar or weak coffee as Agar?  It's about the only consistent carbohydrate source I can get my hands on besides paper products





I really highly doubt that.  I've used corn starch, grain water, oat flour, potato flakes, potato water, table sugar, cane sugar, powdered dextrose, light malt extract, Chinese maltose syrup, Karo and maple syrup.  I'm sure you can find something sweet.  :grin:  If you like, you can use a dog's breakfast of ingredients.  Or, if you feed your dog kibble, you can use a dog's breakfast FOR ingredients.

Quote:

hoffzl01 said:
Keep in mind...even basic laboratory sterilization is close to impossible, as I live and work in dusty sandy tents.




Do you have a stove?  If not, a hot plate?  If not, a grill?  With a little skill you can use a PC on any of those, but a coffee machine just won't do.


Quote:

hoffzl01 said:
-  I want to practice my technique for isolating strains, but that might have to wait until I get home...as I imagine I would really be practicing my technique for managing stress as my plates get continuously contaminated.




No, you can do this.  Look up pictures of still air boxes, and figure out how to rig one up out of a cardboard box, saran wrap and duct tape. 

Quote:

hoffzl01 said:
- If I can make successful agar plates, I want to try store bought microwave popcorn as the substrate for grain spawn.  We have more than a small country could ever eat.  I can use empty bottled water containers, and coffee cans as "jars". Ideas?




Those containers won't hold up to the heat necessary to properly sterilize.  Popcorn is a pretty bad spawn, but I've used it and it can work.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: Psilicon]
    #21164954 - 01/22/15 08:58 PM (9 years, 11 months ago)

yeah sorry for the length of the post.  All good points Griegen. 

Quote:

FYI, we also have a gourmet and edibles section, where you can get advice more tailored to the species you're looking to grow, but I think on the whole we're adequate to answer your questions about oysters and agar.




I considered going there, but seeing as I'm restricted to what's available, I didn't care much for what's "proper" for the species.  I'm gonna try to make it do what I want.  But nature has a way of ruining plans.

I can work with that list of ingredients you've used for agar.  I have a 40 square foot shelf of nothing but snacks and sweets sitting behind me.  I can probably even track down some instant potato flakes.  I hadn't considered that.  I appreciate your input 

I dont think I'm going to be able to get a pressure cooker.  There is a grill available, but I was trying to avoid the inevitable questions that would raise with my superiors.  "Does anyone know why Sarge is boiling 2 boxes of potato flakes and dog food on our grill?"  Hot plates are out of the questions here.  Something about fire hazards and death/destruction...whatever.  :smile:

That being said, I agree with you that I'm going to have to find something better than the coffee maker.  Or accept a very high amount of contam loss.  I'm not looking to start a mold factory in the desert though.

If you've used popcorn for grain spawn before with success, I will try it.  I dont mind if the run is slower, as long as the myc can still grow healthy on it.  I've run through the list of supplements, and there's not many I'm going to be able to acquire easily.  customs can be a PiTA

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OfflinetaGyo
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21164979 - 01/22/15 09:02 PM (9 years, 11 months ago)

IMO get something else. Popcorn has a mess of problems that other grains don't have but VDG is giving you solid advice :thumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

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OfflinePsilicon
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21165106 - 01/22/15 09:27 PM (9 years, 11 months ago)

Sorry, man, but to do agar and grain work, you're going to need to sterilize, and for our purposes that means autoclaving or pressure cooking. No matter where you are, they sell pressure cookers there.  If you PM me your general location, I might be able to help you track down something.  If not, then our hands are kind of tied.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlinemushpunx
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Re: Freedom Fungus - First time grow needs opinions [Re: Psilicon]
    #21165308 - 01/22/15 10:13 PM (9 years, 11 months ago)

Yea all these guys are right. You can pasturize substrate in a pot, and if you have a pot with a lid you can steam sterilize BRF. But not agar or grain spawn.

Well, actually Ive read you can sterilize grain spawm w steam but it takes about 8 hours. If your absolutely reluctant *not saying you are* to shell out sixty bux for a starter PC you could look into this maybe


--------------------

Amateur Mycologists United
AMU Q&A

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: mushpunx]
    #21165791 - 01/23/15 01:40 AM (9 years, 11 months ago)

Awesome responses all.  I really appreciate it.  Let me try to sum up

1) if I want to go agar or grain spawn...sterilize (pressure cooker or autoclave) or dont do it.
2) I MIGHT get away with going cardboard to bulk substrate, but not likely.  And If I got away with it...it wouldn't be manageable long term.  i.e. re-spawns from younger cultures.
3) I need a hood...which I can manage.  I have plenty of blower motors laying around and plenty of building materials. 
4) My substrate choices COULD work, but probably wont yield optimum results.

Just for full disclosure...despite all of this excellent advice.  You know i'm trying it anyways.  the process is fascinating.  and I'm going to have some fun with it.

I'm not "reluctant" to get a pressure cooker at all mushpunx...I just dont have a reliable source of heating it.  However, we have portable diesel engine driven heaters...that can put out 250 degrees.  I'm going to try to build a steam box for pasteurization.  mount the output of that thing directly to the box, keep the contents moist...and see what happens.  But again...you guys seem to suggest that still wont be good enough for agar/grain.

Excellent feedback.  saved me alot of time already.


--------------------
He's not the sharpest cookie in the cupboard.  What?

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InvisibleTheEaglesGift
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21165975 - 01/23/15 03:58 AM (9 years, 11 months ago)

Buy a hot plate off amazing for your PC.

Cadco brand. They're beast.

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Offlinemushpunx
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Re: Freedom Fungus - First time grow needs opinions [Re: TheEaglesGift]
    #21166609 - 01/23/15 09:22 AM (9 years, 11 months ago)

What's a steam box? I guess it depends on what type/how much substrate you need to pasturize.


As far as a hood goes dude, you dont *need* it. Honestly its more of a luxury. I mean if you have the means build one! I did and it's the best!

But most growers on here, many with way more skill and experience tham me use a Still Air Box. You can make one in ten minutes, for 10 dollars, and you can do all your grain to grain or agar work in there. Anything you would do in front of a hood. I say start w this, learm stechnique and in spare time work on a hood!


--------------------

Amateur Mycologists United
AMU Q&A

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: mushpunx]
    #21167764 - 01/23/15 03:29 PM (9 years, 11 months ago)

Steam Box

Instead of using steam from the pressure cooker.  I can use either a gas turbine generator (1400 degrees exhaust temperature) or a portable ground heater (275 degree air) to heat water and direct the steam with fuel line or similar tubing.  When we have down time I'll find junk parts to put something together.  It's amazing the amount of stuff people throw away.

I'll go wit the still air box mushpunx.  If you think it sufficient.  I ordered some birdseed for grain spawn.  And I ordered some hard wood fuel pellets, gypsum, and wheat bran...going to play around with that and the trash cardboard to find a good bulk substrate.  I also might just skip the grain spawn all together, and run clones out on cardboard, and go straight to Unicorn bags with bulk substrate.  Still deciding.

I'm also still concerned with how I'm going to do spore inoculations.  I'll have to work on the pressure cooker/hot plate setup.  Even though I'm not supposed to have it.  I can clone for a little while, but I'm not sure when senescence is going to set in.  If it's a trash strain I'm getting in the kit, I could see problems in the first clone I do.  Who knows.

Oh...and for anyone wondering how I have so much time being deployed...I'm a mechanic.  If I have a lot of free time, it means I've done my job well.  :p

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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21167880 - 01/23/15 04:06 PM (9 years, 11 months ago)

Yea dude in this hobby simpler is usually better. The most important thing is to be able to make clean incoculamt and clean spawn.

And for the most part that entails agar work, good sterile techniaue, good grain prep and pressure cooker cycles.

Large scale pasturization is accheviable in a bunch of ways and its the easier part. Good clean grain spawn is everything


--------------------

Amateur Mycologists United
AMU Q&A

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OfflinePsilicon
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Re: Freedom Fungus - First time grow needs opinions [Re: mushpunx]
    #21168080 - 01/23/15 05:04 PM (9 years, 11 months ago)

:whathesaid:  With clean grain spawn you can get away with a lot.  Without it you can't get away with much.  Admittedly oysters are a hardier species, but superspawning even that species doesn't tend to work out well.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: Psilicon]
    #21224047 - 02/04/15 12:07 AM (9 years, 11 months ago)

Not trying to bump.  But I wanted to document this.  If anything it may show just how robust mycellium can be.

My supplies arrived...it's not much but it's a start.

So the Blue Oyster grow kit I ordered is effectively a bag of fully colonized supplemented sawdust substrate.  With instructions to cut an "X" in the bag,
soak it for 8 hours, then keep it moist twice a day until it fruits.  Pretty standard.  I didn't feel like waiting for it to fruit to have fun....so......

I understand sterilization is a HUGE problem in this setup...but I'm working with what I have.  I have not obtained a pressure cooker yet, and
fully understand that is the best...if not the ONLY way, to be sure.  But I'm bored...so here we go.




Procedure 1:  Agar transfer
*Prepared agar
- One package of ramen (just the noodles!) boiled in 16 ounces of water in the microwave
- drain and filter to obtain the resulting liquid.  (carbohydrate/starch)

- withdraw and measure required amount of liquid.  I was only doing about 4 plates so I withdrew 100ml

- placed in separate plastic container, added 5% by volume "Heinz Breakfast Syrup" (mostly corn syrup and sugar, with a little salt)

- added appropriate amount of agar (10g per 500ml = 2tsp per 500ml = 2g per 100ml)
- returned to microwave until just before boiling (small bubbles appear and stay, occasionally rise to surface)  I didn't want to risk
carmelizing the sugars, but gain some semblance of sterilization.
* Poured agar plates
- Allowed agar mixture to cool for 2 minutes before sucking it into 100ml syringe, and delivering to plates that were previously
wiped down with isopropyl alcohol and left covered.
- Performed under cardboard cover...but I wouldn't call it a still air box.  Just to keep heavy settling particles out.


- Allowed plates to cool and set for 15 minutes.  No large condensation drops and firmness was correct.  I considered it a success so far
- Removed small (pencil eraser sized) pieces of mycellium from grow kit using alcohol sterilized utility knife.
- Quickly lift petri dish lid and deposit mycellium...gently pressing into agar.  Close lid.  Sealed with 1 inch wide stip of aluminum
foil all around the edge of dish.
- Re-sterilize knife, repeat.
- Stored in dark corner (still some ambient light).


Procedure 2:  Liquid cultures
Breakfast Syrup LC
- prepared 500ml bottled water in plastic container.
- Added 5% by volume "Heinz Breakfast Syrup
- Made two 3/8" holes in plastic container lid.
- Filled holes with polyfill

- Sealed container and microwaved to slow boil 6-7 minutes
- Allowed container to return to room temp.  leave lid on!
- Alcohol sterilized utility knife and removed small piece of mycellium from grow kit
- slightly cracked lid and deposited mycellium.  slow swirl to mix thoroughly. 
- sealed container lid edges with alcohol rubbed aluminum foil.

Sugar/salt LC
- prepared 500ml bottled water in plastic container
- Added 5% by volume "N'Joy Pure Sugar" (I had it laying around)
- Added small pinch of salt to inhibit bacterial growth
- Microwaved to slow boil
- inoculated same as above in similar container as above
- Sealed container in same manner

Procedure 3: Cardboard Clone
- Needed to expand the mycellium from the initial grow kit using cardboard
- Soaked rougly 6" x 3" pieces of cardboard in 3% hydrogen peroxide for 15 minutes
- drained peroxide and rinsed cardboard with bottled water, drained water
- Ensured loose water was shaken from cardboard
- Gently peeled back layers to expose the corrugated section inside each piece
- Placed nice thick mycellium from top of grow kit onto corrugated section, replaced top piece of cardboard
- Repeat for each piece of cardboard
- Stacked them in plastic container with gas exchange holes (poly filled 3/8 holes on lid) and sealed with aluminum foil
- Placed in dark corner at rougly 70 degrees.

NOTES: 
- Obviously I am worried about sterilization.  I expect to grow mostly contaminates...but I am insanely curious to see if it can be done.
When I return home I will do the whole lab setup.  Can't do it here.
- I am worried about the preservatives in the breakfast syrup interfering with the mycellium.  But I was unable to find honey, karo, or
any of the traditional additives to make agar and liquid cultures.  That's why I attempted the sugar/salt LC.  Keep it more natural.
- Just waiting now to see what happens.  We will call this Day 0. (February 2, 2015)

Day 3 report - Agar plates are showing signs of growth.
(The brown and black flecks are just substrate that was sticking to the mycellium.  sawdust pieces, as well as a couple pieces of ramen noodle)  Makes me believe the recipe for the agar is good, but may still contaminate.  The mycellium is actually growing up and out of the small impression left from pressing it in.  Breakfast syrup liquid culture is showing signs of growth as well.  The mycellium clumps floating in it are getting extremely "fuzzy" around the edges.  I doubt cardboard is doing anything yet, but I dont want to open it and risk contam until at least 2 weeks.

Day 5 - Plates still growing.  breakfast syrup LC is doing well.  Cardboard clone is growing well without contam.  Cardboard inoculated with half the grow kit is performing

Edited by hoffzl01 (02/05/15 05:59 PM)

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21224089 - 02/04/15 12:23 AM (9 years, 11 months ago)

Dude, that oyster kit is not sterile. Trust me.  Spawn the kit to cardboard in a bigger bucket lined with a shopping bag, let it colonize, and then do this.






--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Edited by NumeroEno (02/04/15 12:24 AM)

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21224098 - 02/04/15 12:26 AM (9 years, 11 months ago)

IMO if you want to get a clean culture from that kit, take a print and clean that up on agar. That's what I'm gonna do.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21224113 - 02/04/15 12:30 AM (9 years, 11 months ago)

I like that idea.  I'm gonna have to do something.  The kit is the only thing NOT growing.  It's like 10% humidity all the time where I'm at.  So I can't keep the thing moist even in a clear plastic bag.  They mycellium is already getting pretty dried out.  A bucket with plastic over it might be the way to go.  Thanks for the input. I need to get some fruits off of it before my tour is over, and obviously I can't get any prints until I can get something to fruit.  when/If the plates grow out, I'll need to have a solid plan for the bulk substrate/fruiting.  But yeah, ultimately I want some spores to play with.  I want to do LC's with multispore. I only got the kit to get me my initial fruits.


--------------------
He's not the sharpest cookie in the cupboard.  What?

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21224136 - 02/04/15 12:39 AM (9 years, 11 months ago)

Alright, I thought about it for 20 minutes..tomorrow I'll transfer that kit to a nice sized tupperware with some cardboard.  break the kit up in that thing.  I can control the moisture better, and still get some light and FAE when it fully colonizes.  good idea Numero.  Maybe I'll make a tiny version of a Monotub with some 4 quart tupperware.  put larger holes in it that I can tape shut during colonization.  then uncover/poly-fill when it's time to fruit.  keep more moisture in but still get FAE.


--------------------
He's not the sharpest cookie in the cupboard.  What?

Edited by hoffzl01 (02/04/15 12:46 AM)

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21224169 - 02/04/15 12:55 AM (9 years, 11 months ago)

Oysters don't do very well in monotubs. Here's what I did with my kit:

I fruited the first flush out of the kit per the instructions.
I shredded, soaked, and pasteurized a pretty big cardboard box
I got a 2 gallon bucket, drilled a hole in the lid and stuffed it with poly, and lined it with a plain old plastic grocery bag
I then put alternating layers of cardboard and the crumbled (and dunked) sawdust block in the bucket until it was full 
I let it colonize for about 2 weeks
I took the shopping bag out of the bucket, slit the bag in places that looked like good pinning sites, and put it in my greenhouse to fruit

I'll post another pic in the morning. They grow so fast!


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21224200 - 02/04/15 01:14 AM (9 years, 11 months ago)

I can re-produce that process all the way until the last step.  If I remove it in a plastic bag and slit for pin sites.  That entire block will be bone dry in 2 days no matter how much I mist. But I like the general idea.


--------------------
He's not the sharpest cookie in the cupboard.  What?

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231160 - 02/05/15 03:34 PM (9 years, 11 months ago)

Took your advice part way Numero.  I bought a bucket here on base that has a wringer attachement with it.  Just so happens that the wringer attachment keeps the block about 2 inches from the bottom.  So I put an inch of water in the bottom and covered with a clear plastic bag.  Keeps the bucket humid inside, and  lets light in.  I just uncover about 4-5 times a day for fresh air exchange.  The block started pinning within 12 hours.  Nice grey/blue pins.  Very cool




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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231243 - 02/05/15 03:48 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
I can re-produce that process all the way until the last step.  If I remove it in a plastic bag and slit for pin sites.  That entire block will be bone dry in 2 days no matter how much I mist. But I like the general idea.




I feel your pain. I live in the desert. I run a mini greenhouse with a USB powered fan and humidifier. I've only been running it for about a week but it works well. I have a bunch of king oyster on agar that I'm gonna do a bunch of bottles with. I'm gonna try to respawn my block to sawdust in a spawn bag after I pull this flush, but I'm eventually going to do all bottles in my greenhouse. I just hope the oysters don't totally overrun my cubes, being in the same fruiting space.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21231322 - 02/05/15 04:00 PM (9 years, 11 months ago)

You shouldn't have any "over-run" problems if you stick to a couple/three flushes for each cubes and oysters.  Then toss the substrate and go back to your masters (grain or lc) for some new bottles.  Cross-spores wont have any time to germinate

I took half of that grow kit that was kind of stalling out and mixed it in with cardboard(and a couple other things) in a large tupperware (with small gas exchange holes), to see if my intended substrate will work out.  It took off in less than a day and is already visibly colonizing.  I'm very surprised that I havn't had any contams even on my plates yet.  I didn't even come close to proper sterilization technique.  I mean I washed my hands and wiped all tools/containers with alcohol, but that's it.

Your greenhouse sounds cool.  When I get home I'm setting up a 4 shelf, plastic covered one.  I intend to fully automate with Arduino micro-controller and sensors. Humidifier and air pump. I already have all the parts waiting at home.  (It is surprisingly cheap for a setup like that) Just have to get outta here.  I might need some advice when that time comes around :p


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231506 - 02/05/15 04:37 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
Hey all - First time cultivator here.  I've been reading your boards for weeks and read most of Stamet's works...ready to try my hand.

So I'm deployed to the sandy and sunny part of our planet, and decided I wanted to try growing some oyster mushrooms here.  I ordered a grow kit instead of spawn or spores, to get my initial sample from.  I've also ordered some basic materials (agar, petri dishes, scalpals, etc)
- *** What do you guys/gals think about the feasibility of using cooked ramen broth supplemented with sugar or weak coffee as Agar?  It's about the only consistent carbohydrate source I can get my hands on besides paper products
- *** I have no idea how to do any basic sterilization for the agar medium.  we have to get creative here!  I have a coffee maker and a lighter. :smile:  mushroom McGyver style.


Thanks!


Look up steam sterillisation as far as agar nutrients You probably could use Ramen noodle broth not the spices just the noodles just save the water and throw the noodles away and maybe some very dilute coffee like 1 cup watered down into 6 or 7 Coffee is very acidic but you could soak your cardboard in the same mix like 1 cup of dilute coffee to 2 cups noodle water. And no you can't use microwave popcorn. Someone on here actually grew shrooms on pasta. Do you have access to straw or hay? If so do a search on straw logs for oysters. Oysters will grow on almost anything they are just so agressive colinizers. Just read then read some more and after that read even more.:manofapproval:


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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21231668 - 02/05/15 05:19 PM (9 years, 11 months ago)

you got it rooot.  I used Ramen noodle broth, supplemented with breakfast syrup at 5% volume.  I left the coffee out as I was worried about resulting PH level.  Seems to be working.  Growth at 4-5 days, no contams yet.  Seems to be a little on the slow side...but working.  The chunky stuff in the middle is the original sawdust substrate the mycellium came on.



I can't get straw here, but supplemented cardboard is working well as a substrate so far  (not pasteurized...h202 soak for 1 hour, then rinsed and microwaved for 5 minutes)


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231695 - 02/05/15 05:26 PM (9 years, 11 months ago)

Very nice log my friend glad to see you were successful.

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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231744 - 02/05/15 05:36 PM (9 years, 11 months ago)

Keep going man if you got myc out of nuked agar build a SAB if you haven't already and cut a wedge from that agar And noc up some jars/bottles of cardboard. You got any dead vegetation at all? you know like desert plants anything kinda woody but not a tree? you could try that chopped up instead of straw. That or maybe palm fronds chopped up. Keep posting pics this is possibly the most Ghetto grow ever. You would be the king of teks. :thatsayes:


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21231934 - 02/05/15 06:11 PM (9 years, 11 months ago)

I'll keep the pics coming as it progresses.  The plates are growing slow enough that I'm pretty confident bacteria will win before it colonizes...but who knows!
The Desert Ghetto Tek would be a magnificent work  :thumbup:

I'm pretty confident in the cardboard though.  I dont think this area of the world even knows what mold is.  The humidity is a problem...but that might actually work to my benefit when fighting contams.  I'm glad Numero got me on the bucket idea.  That might have solved the humidity issue when fruiting. 

As long as I still have some living mycellium, I will keep trying methods.  I leave for home in about 9 weeks...so I'm hoping to get some fruits before then.

I havn't seen a plant other than a couple dead tumbling shrubs in 4 months.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21231938 - 02/05/15 06:11 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
You shouldn't have any "over-run" problems if you stick to a couple/three flushes for each cubes and oysters.  Then toss the substrate and go back to your masters (grain or lc) for some new bottles.  Cross-spores wont have any time to germinate

I took half of that grow kit that was kind of stalling out and mixed it in with cardboard(and a couple other things) in a large tupperware (with small gas exchange holes), to see if my intended substrate will work out.  It took off in less than a day and is already visibly colonizing.  I'm very surprised that I havn't had any contams even on my plates yet.  I didn't even come close to proper sterilization technique.  I mean I washed my hands and wiped all tools/containers with alcohol, but that's it.

Your greenhouse sounds cool.  When I get home I'm setting up a 4 shelf, plastic covered one.  I intend to fully automate with Arduino micro-controller and sensors. Humidifier and air pump. I already have all the parts waiting at home.  (It is surprisingly cheap for a setup like that) Just have to get outta here.  I might need some advice when that time comes around :p




Wow! That's badass. I just have my stuff on a timer to run for 30 minutes at 4 hour intervals. The only reason I mentioned my oysters contaminating my cubes is because MudaFuka posted some great pics of oysters that grew out of his cube bottles. They bruised blue, lol.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21231953 - 02/05/15 06:14 PM (9 years, 11 months ago)

On a side note, ghetto oyster growing is awesome. I'm gonna have to try the ramen agar. My kings on GLMEA/potato starch agar are doing really well.  Can't wait to get those in bottles.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21231984 - 02/05/15 06:19 PM (9 years, 11 months ago)

Quote:


Wow! That's badass. I just have my stuff on a timer to run for 30 minutes at 4 hour intervals. The only reason I mentioned my oysters contaminating my cubes is because MudaFuka posted some great pics of oysters that grew out of his cube bottles. They bruised blue, lol.




Psychadelic AND Delicious....perfect.  :crazy2:


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232171 - 02/05/15 06:53 PM (9 years, 11 months ago)

Oh and by the way...here's the vegetation status where I'm at.  It looks like this pretty much everywhere.  Even food for mushrooms is hard to find.  I'm not really sure why anyone decided this was a decent place to live.



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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232226 - 02/05/15 07:04 PM (9 years, 11 months ago)

Man you have enough myc right meow to take a wedge, do like 6 bottles cardboard or what ever as a test run. Hoping it all goes well man. :thumbup: Now all you need is spawn.:thatsayes: You could requisition a bale of straw. :lol:


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Edited by r00tuuu123 (02/05/15 07:10 PM)

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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21232251 - 02/05/15 07:10 PM (9 years, 11 months ago)

Maybe straw is in the Federal Stock System.  I'll order up a bale and it will be here in 1-2 years.  :grin:


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232312 - 02/05/15 07:23 PM (9 years, 11 months ago)

You can also use spent coffee grounds and sawdust. I'm experimenting still but muda has done some awesome oyster grows from bottles made with LI. I'm just doing stuff that's already been done to death my muda and eatyualive, among others, but man is it fun.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232331 - 02/05/15 07:27 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
Maybe straw is in the Federal Stock System.  I'll order up a bale and it will be here in 1-2 years.  :grin:



:lol: Maybe order some old kapok life presevers from the Navy Lol they would probably get there in a few days. :lolsy: I can see it now Quaetermaster looking at it Life preservers in a desert? Fuck it send em.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21232366 - 02/05/15 07:35 PM (9 years, 11 months ago)

Quote:

I can see it now Quaetermaster looking at it Life preservers in a desert? Fuck it send em.




I'm sure they've sent stranger things to stranger places lol.  I'd still love to see the look on his/her face


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232514 - 02/05/15 08:03 PM (9 years, 11 months ago)

When I get home, I'm pretty sure I want to use LI and LC TEK's.  I need to run some tests and figure out what's fastest, least contam, highest yield/spawn of the following:

Plate -> Grain Master -> Grain Workers -> Bulk Bags

Plate -> Grain Master -> Slurry -> Grain Workers -> Bulk Bags
Plate -> Grain Master -> Slurry -> Bulk Bags

Plate -> Liquid Culture -> Grain Workers -> Bulk Bags
Plate -> Liquid Culture -> Bulk Bags

Plate -> PF Cake -> Slurry -> Grain Workers -> Bulk Bags
Plate -> PF Cake -> Slurry -> Bulk Bags

I dont want to expand more than 3-4 times from the slant/plate, but want do do it the most efficient way possible.  I've been impressed by some of the reading on slurries (LI), but obviously have no experience with it


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232661 - 02/05/15 08:33 PM (9 years, 11 months ago)

I would make LI with clean agar. You can't really tell if LC is clean until it's too late to matter.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21232760 - 02/05/15 08:52 PM (9 years, 11 months ago)

So you like straight Agar -> LI -> Grain or Bulk?  LI being just mycellium water...Agar slurry if you want to call it that?

Sounds decent.  Agar to make sure it's clean, liquid to make it "go further" and colonize faster.  Straight to grain workers for a few days before bag substrate would probably work for me.

I'll compare that to the other closely related method of spawning agar out to grain or a cake and THEN making a liquid for further spreading. See what comes out on top in terms of speed and spawn material.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232978 - 02/05/15 09:45 PM (9 years, 11 months ago)

I honestly haven't done it this way personally yet, but I've seen such great results with it and it seems so simple. Check out mudafuka's bottle tek thread.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233142 - 02/05/15 10:40 PM (9 years, 11 months ago)

Mods please don't move this to edibles where it will languish in obscurity This guy knows his shit. As they say bullshit walks


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21233153 - 02/05/15 10:46 PM (9 years, 11 months ago)

I've only been doing oysters for a month or so. Not much of my knowledge is practical, but there's a lot of good work done by others that I'm referring to. My only real experience with oysters is the growkit spawned to cardboard.

But yeah, I have a few threads on the edible board. Not much activity there. Mush cult gets so much more traffic. I don't make the rules, but I see no reason to not talk about edibles in mush cult on occasion.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233236 - 02/05/15 11:17 PM (9 years, 11 months ago)

Agreed.  Most of these techniques would apply to the basic variety of our more "magical" friends.  (From my reading and others research)  In fact, I will apply some of these techniques in a very magical way, when I'm not in such a public setting.  I didn't even realize it was in the wrong board.  But maybe the results will keep it here. 

This is the other half of that grow kit Numero. 



Crumbled in with shredded cardboard and various card stock boxes from parts that come here. (Cardboard water was supplemented) I'm trying to avoid opening it, but the temptation is almost irresistible.  I need to stop...so I can keep the carbon dioxide levels up while it colonizes.  It's starting to take over the cardboard very aggressively, and in a week or so, I'm going to slave it out to about 6 ziploc bags of cardboard. (Assuming it doesn't contaminate from me opening it...doh!)

Your bucket idea is the fruiting chamber.  The ziploc bags will have enough substrate that I can fit the resulting block into the bucket one at a time for fruiting.



These pictures are the same block using that bucket.  Less than 7 hours apart.  It's pretty ridiculous how fast these things grow once they take off.

0100 my time   

0800 my time   


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21233256 - 02/05/15 11:22 PM (9 years, 11 months ago)

Nice. I'm printing this big one to grow out on agar. Then I'm gonna eat it. Probably gonna spawn the substrate to pasteurized sawdust bottles after that :cool:



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Edited by NumeroEno (02/05/15 11:23 PM)

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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233455 - 02/06/15 12:28 AM (9 years, 11 months ago)

you eat that thing man.  you eat it good.  The kit was a pretty cost effective way to get spores/mycellium/clones.  I'd do it that way again if I had to.  At least it's not a dead spore syringe that you have to wonder about for 2 weeks.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21233918 - 02/06/15 05:45 AM (9 years, 11 months ago)

hoffz you ever cook oysters?  That thing will turn to about the size of a 50 cent piece when its done cooking. :lol: but all in all you're on the right track. Good lookin out. (as the kids used to say) All hail the king of Ghetto teks. I wan't a print when you get that far. :thatsayes::manofapproval:


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21235714 - 02/06/15 03:26 PM (9 years, 11 months ago)

You'll get a print!  I'm gonna put some of this desert sand in a baggie with it, and slap an American Flag on the package for yah.  haha


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21244474 - 02/08/15 04:01 PM (9 years, 11 months ago)

*** UPDATE ***
2-9-2015

The grow kit is doing well.  It appears from the long stipe and small caps that I may have been a bit short on the FAE and possibly light...but I've done what I can.  It's tough riding that crazy fine line between fresh air and humidity when the RH of the air is 10%.  I used our magnifying bench light to give the 12/12 light as the sun here is a bit too intense.  It's only 3000K...so not quite high enough color temperature, but it's doing the job.  When I harvest, I will take multiple spore prints.  I am going to store some in water solution in vials, plate some, and LC some.  I'm not even going to attempt isolating until I get home.  But at least I will have a few vials of multispore I can start with.  No need to buy more.



The other half of the grow kit that I expanded to cardboard is colonizing ridiculous fast.  I'm going to give it 3-4 days to consolidate and then fruit it right in that plastic container by removing the lid and placing foil with multiple holes right on top the substrate pretty tightly.  These are just for eating.  Nom Noms.

I may try to use this substrate when I get home for bulk.  It is doing that well.  Since it was a kit, I dont know exactly what's in it..but doing a little research it appears to be hardwood sawdust and small amount of coffee grounds/wheat bran, with my added cardboard that was soaked in weak coffee and syrup solution.  Its worth further experiments, but seems to be a pretty basic recipe. I crumbled about 1/2 pint of colonized substrate into that only 3 days ago.



The Agar plates are coming along, although slowly.  If I do it again, I may add a slightly higher concentration of syrup 7.5 - 10%, or just use plain sugar at 5%.  I lost one plate out of four to mold, but I will accept 25% contam loss on plates that were not pressure cooked, and basically filled in open air.  I plan to put the plates into an LI (agar slurry) when they are fully colonized and inoculate cardboard.  No sectoring on the plates, but that's to be expected since it was started from already dikaryotic fully colonized mycellium. 



I was thinking about this last night, and I have to ask..but this question may be more suited for the advanced mycology board.  In the genetics of fungi, does taking a spore print from a fruit with certain desired traits increase the likelihood of re-producing those traits in one of the later isolates of that multispore?  As opposed to taking ANY random fruit from that block. 

Doesn't that fruit (even if it has certain desirable traits), still just have the same genetic make-up in the spores it produces, that all of the fruits from that same mycellium have?

I have seen people mention taking prints from fruits that had a trait they liked in an attempt to re-produce that trait (through isolation and lots of trial)...but I thought perhaps ANY fruit from that same mycellium (same block) would probably provide the same genetic code necessary to re-produce the desirable trait that appeared in that block. The fruit (no matter what genetic traits it presented) doesn't determine what genetics are in the spores it produces..right?

Just curious for when I get home and start isolating.  One fruit is much more "blue" than the rest and was thinking about why.  I'm not an expert...just a curious hobbyist.


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Edited by hoffzl01 (02/08/15 04:16 PM)

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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21244953 - 02/08/15 05:52 PM (9 years, 11 months ago)

Mine isn't doing all that well ATM. I'm kind of using it as a gauge to dial in my greenhouse for when this batch of cubes I have going is ready to fruit. I also have a small bottle of cubes currently fruiting in there. I think I'm gonna try spawning this block to sawdust in a mycobag and dunk it really well before fruiting. This current flush is pretty disappointing, but I will at least get some prints from it.


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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21244984 - 02/08/15 05:58 PM (9 years, 11 months ago)

Yeah, I noticed on mine when I broke it apart...that it was very very dry inside.  Even after the initial 12 hour soak that it called for.  The mycellium was so dense that the soak effectively did nothing.  I had to poke multiple holes with a fork all over the block and keep it very very moist.  I think you'll do just fine if you spawn it out and soak it well.  It seems to be a pretty healthy and aggressive strain.  It might be my "eating" strain when I get home.  I want to develop my own from wild samples...but if these taste good I'll keep them going.

I dont think this strain will get very big though.  One of the caps is already starting to go concave and it's only about an inch across.  AGain, could be FAE or humidity.  that one is getting printed as soon as it goes flat.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245011 - 02/08/15 06:06 PM (9 years, 11 months ago)

They can get pretty big. Here's a pic of the first flush I got out of the kit. I didn't dunk it this time and I think that's why this flush is pretty disappointing. I think it's also been expanded a whole bunch which is why I want to start some prints on agar. The one clone I took looked great but some trich snuck in and the grain jar went green.



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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245049 - 02/08/15 06:14 PM (9 years, 11 months ago)

Damnnnn...nice looking fruits.  I think mine is stalling out.  But I'll be patient and let it go for a few days.  I dont think they like growing off the top like I have it set up.  I've read that oysters dont take well to horizontal fruiting.  So strange that they can tell.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245075 - 02/08/15 06:22 PM (9 years, 11 months ago)

Yeah those were tasty. I wish I had printed one of them! I have a lot of king oysters going right now too, but I'm far from done with these. Next time I spawn them I'm gonna give the bag at least a week to consolidate and also do a 24 hour dunk. I'm really excited to do some multispore grows with these, though.


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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245168 - 02/08/15 06:42 PM (9 years, 11 months ago)

I really like the deep blue color when they pin and young fruits.  It's aesthetically pleasing.  I'm excited as well to play around with these multispores.  I'm just excited in general that my first adventure after months of reading is working out.  Just 8 weeks until I go home and set up my man shroom cave.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245335 - 02/08/15 07:40 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
I really like the deep blue color when they pin and young fruits.  It's aesthetically pleasing.  I'm excited as well to play around with these multispores.  I'm just excited in general that my first adventure after months of reading is working out.  Just 8 weeks until I go home and set up my man shroom cave.



  you've done well Getting fruits to grow in a most inhospitable environment. Keep making prints and agar plates! Those have to be some hardy oysters. Don't know what area of the country you will be coming to but damn sure better than the desert. :thumbup: Oh and Imma hold you to that print when the weather breaks in a few weeks I might have some wild elm oyster prints to trade plus some really good other prints.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21245409 - 02/08/15 07:56 PM (9 years, 11 months ago)

Well I think I screwed this current flush up by not dunking beforehand. I decided to pull the few fruits off and dunk it because it was so dry. I had a hard time keeping the block submerged under a gallon jug of water, and there were air bubbles coming out of the sub for a while :facepalm:

I'm gonna put it back into fruiting conditions in about a week, because I want to give it more time to eat the sub. Gonna dunk it again before fruiting too.


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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245535 - 02/08/15 08:24 PM (9 years, 11 months ago)

Quote:

NumeroEno said:
Well I think I screwed this current flush up by not dunking beforehand. I decided to pull the few fruits off and dunk it because it was so dry. I had a hard time keeping the block submerged under a gallon jug of water, and there were air bubbles coming out of the sub for a while :facepalm:

I'm gonna put it back into fruiting conditions in about a week, because I want to give it more time to eat the sub. Gonna dunk it again before fruiting too.



You don't need to do all that with oysters just watter it with a watering can and wait a few days and it'll flush again till there's no more nutrient to feed it.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21245558 - 02/08/15 08:32 PM (9 years, 11 months ago)

Well I can't really pour a watering can all over my greenhouse so that's why I'm dunking it.

You're right though. I guess there's no real reason to put in back into colonizing conditions right now. I'm just gonna let it soak overnight and put it back into fruiting conditions.


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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245848 - 02/08/15 09:42 PM (9 years, 11 months ago)



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Re: Freedom Fungus - First time grow needs opinions [Re: Grey]
    #21245978 - 02/08/15 10:23 PM (9 years, 11 months ago)

Thanks classy.  There is plenty of toilet paper around!  I'll check that out.  Root I'm heading back to beautiful Indiana when I'm done here.  At least it stays plenty humid there.  I will save a print for ya...even if it's just one from the first flush on the kit. But hopefully it will be one from the multi-spore I manage to fruit out here.  Then you can truly say you have a print of an oyster mushroom that went from spore to fruit all in the desert during our country's most recent "conflict".


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21259051 - 02/11/15 03:00 PM (9 years, 11 months ago)

:manofapproval::raisemyglass: keep up the good work.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21269311 - 02/13/15 02:43 PM (9 years, 10 months ago)

HOFFZ I dont know why this didn't occur to me earlier but here goes nothing. Theres camels in the desert if you can get ahold of say a 5 galon pail of camel manure suplement your cardboard with that that myc would probably grow crazy fast.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21273634 - 02/14/15 03:10 PM (9 years, 10 months ago)

I bet they would love that "shhhiiit".  They would grow like me watching a Scarlett Johansson film.  **Rimshot** But seriously, I'm not touching Camel Poooo.  I've had my Hep vaccines, but I'm still not taking that chance. :grin:  But that's a damn fine idea, and quite creative thinking.

My fruiting block in the bucket stalled out with caps about 1" diameter.  I had to clean the block off and put it in a plastic bag to rest.  I'm going to try fruiting it again in a couple weeks.  I tried to get some spores, but I believe the fruits were not developed enough to produce.  I'm positive I didn't provide enough light and not high enough oxygen levels. I had to keep it covered so much to keep the humidity up, that I think I choked it out. .

But Not to worry!  I still have 3 blocks that I expanded from that first one that are still colonizing healthily. 

So...which do you think I should try when it comes time to fruit?

1) modify the bucket I'm using into a SGFC, hopefully that will help me win the constant battle between humidity and fresh air.

2) I'm colonizing in rectangular tupperware, when it's 100% and consolidated, put it in a close fitting plastic bag with 1/4" holes all over.  And mist multiple times daily.  (the most recent block is already colonizing in a plastic bag so I can poke holes for fruiting later.)

Not sure which way to go.  I'm going to get you those spores root.  This is going to happen.  I'm very persistent, enough that I can usually overcome my ignorance.

Oh and I added some bran cereal to the cardboard before microwaving in the most recent bag.  That one is growing like crazy.  The ingredients were mostly grain brans of all types and a small amount of sugar.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21273710 - 02/14/15 03:29 PM (9 years, 10 months ago)

Do you have access to poly fill? You could make holes in the bucket and stuff them with poly fill to allow air flow, but trap in moisture. Then just adjust poly fill tighter or looser according to moisture loss. Maybe mist to compensate until you have it dialed in.


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Re: Freedom Fungus - First time grow needs opinions [Re: Grey]
    #21274195 - 02/14/15 06:00 PM (9 years, 10 months ago)

I do have poly-fill.  I bought a $4 pillow at the base exchange that was stuffed with pure poly-fill.  Maybe I'll give that a try for the next round.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21274284 - 02/14/15 06:22 PM (9 years, 10 months ago)

Quote:

hoffzl01 said:
I do have poly-fill.  I bought a $4 pillow at the base exchange that was stuffed with pure poly-fill.  Maybe I'll give that a try for the next round.



The guys with you are probably thinkin you're fuckin nuts. :lol:


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21274321 - 02/14/15 06:36 PM (9 years, 10 months ago)

You hit the nail on the head.  They think I'm half retarded.  I have to hear about it every day.  The only way I'll shut them up, is putting some fresh mushrooms on their burgers in a few weeks.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21279579 - 02/15/15 07:41 PM (9 years, 10 months ago)

hoffz have a look at this thread if you can get rice you can probably pull this off :lol: http://www.shroomery.org/forums/showflat.php/Number/21238376#21238376 I didn't think it would work but dude has gone to colonizing and should be fruiting in 10/14 days.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21280352 - 02/15/15 10:35 PM (9 years, 10 months ago)

That's nice.  I really hope that fruits for him.  I read the whole thread.  I'm really surprised how much success people are having without even basic lab sterilization.  Just overall cleanliness seems to work well enough for small scale.  I'll definitely look for some rice.  We might have that at the base exchange.  I'm not sure.  that's one I havn't tried.

I'm not having any trouble at all getting things to colonize.  A wide variety of things in fact now.  But I'm still having trouble getting things to fruit.  I think I'm going to abandon the bucket.  I dont think enough FAE is happening, the block sits down too low. 

Next round I'm trying stackable tupperware.  the kind that fit tightly into one another.  As most do.  I actually dreamed about that last night.  It was weird.  I'll take some pictures and show you what I mean.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21280443 - 02/15/15 10:46 PM (9 years, 10 months ago)

oh and thanks for keeping an eye out for me.  Anything you guys can get that makes this successful is most appreciated.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21280484 - 02/15/15 10:52 PM (9 years, 10 months ago)

Put some bricks under the block so it can get air that and mist the hell out of it Oysters are rediculously easy I had a bag of oak mulch that was wild colonized slit it open and watered it with a plant watering can when I wanted some for the next day, worked till the bag was spent.
Though i still think you oughta try the Camel poo You're runnin short on time.


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21280522 - 02/15/15 10:58 PM (9 years, 10 months ago)

So here's the next plan.  I'm pretty sure this will only work for oysters as they seem to enjoy growing from pin holes and growing vertically.

- There will be an inner, and an outer tupperware.
- Place holes in the positions shown on the INNER tupperware and place holes on the lid -- then cover the lid holes with Tyvek.



- Fill INNER tupperware with substrate and microwave or pressure cook
- inoculate INNER tupperware.
- Place INNER tupperware into OUTER tupperware...should be a snug fit.  (Same size tupperware i.e stackable kind)



When in place it should look like this



- Seal the edge all around with plastic wrap.  The masking tape in the picture is simulated plastic wrap.



- this should keep moisture loss to minimal while colonizing, but still allow gas exchange
- When 100% colonized and consolidated, break plastic wrap off, and pull inner tub from outer.  Place in Bright light for 12/12, and mist holes multiple times daily.

I'm hoping this will keep enough moisture in the substrate, that the low RH in the air will not affect the fruits as much.  It also simulates current bag tek for Oysters, with something far more re-usable than poking holes in a plastic bag.

It's all just theory at this point.


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21280532 - 02/15/15 11:01 PM (9 years, 10 months ago)

Quote:

r00tuuu123 said:
Put some bricks under the block so it can get air that and mist the hell out of it Oysters are rediculously easy I had a bag of oak mulch that was wild colonized slit it open and watered it with a plant watering can when I wanted some for the next day, worked till the bag was spent.
Though i still think you oughta try the Camel poo You're runnin short on time.





yeah I am running out of time.  7-8 weeks left.  I've got two blocks sitting in fruiting conditions and just wont pop.  I promise I'm misting the fuck out of them.  I dunked both for 24 hours after full colonization, and one has been sitting for 4 days in fruiting condition doing nothing.  I'm guessing it's not going to at all.

Camel poo is so icky though!!! *whiney voice


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21280597 - 02/15/15 11:13 PM (9 years, 10 months ago)

Quit yer bitchen you've touched worse. :lolsy: But seriously dried poo outa the stables should have 0 odor or wetness and is probably mixed in with straw/hay both great for oysters. If your guys didn't think you were nuts before wait till they see you pasteurizing camel shit! :lol:


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21280903 - 02/16/15 12:36 AM (9 years, 10 months ago)

Pasteurizing camel poo while on deployment might earn me a one way ticket to Germany and a visit to the head shrinker :shocked:


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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21324643 - 02/24/15 07:18 PM (9 years, 10 months ago)

What happened to this guy been 8 days since he posted on his grow? Hope he didn't get shot or blowd up!


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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21325251 - 02/24/15 09:08 PM (9 years, 10 months ago)

Yeah, I was thinking that yesterday.


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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: Grey]
    #21345229 - 02/28/15 05:39 PM (9 years, 10 months ago)

not blowed up!  Internet here has been pretty crappy.  So I failed at producing fruit.  I had to throw away the last of the mycellium a couple days ago.  However, things I learned.....

- You can do agar work without laboratory grade sterilization.  I made 20 different plates that myc successfully started crossing...and only lost 3 to contams after a two week period.  All using a microwave and no pressure cooker or even a still air box.  That is very intriguing.

- I believe Oysters need a VERY large amount of Oxygen (fresh air exchange)...and they do not enjoy a "tub" or "covered" setup.  I was forced into using this sub-optimal setup to keep the humidity up in this 10% RH environment...and I dont think it allows enough FAE to promote fruiting.  I got the one block to fruit once...but it quickly stalled out.

- Cardboard works....but I wont use it ever again.  While it's very easy to sterilize, it's just too slow for a substrate.  I tried a variety of "unique" substrates while I was here..and myc ran across most of them.  But cardboard was far and away the slowest.  It doesn't colonize evenly, it's awkward to deal with, requires shredding to quite small pieces, and doesn't hold moisture evenly.  I think there are "waste" products that would be a better fit, since cardboard can easily be recycled by standard methods.

- Obsessing over, and checking your grow every 15 minutes will not make it grow faster.

- Mycology is one of the most inexpensive and enjoyable hobbies I've seen

- Shroomery members are helpful, knowledgeable, and fun people

- I know exactly what I need to do when I get home in 6 weeks.

- Mushrooms will grow on just about anything.  Provide a carbohydrate, a sugar, some basic vitamins/minerals, and keep the ph right....and that stuff will grow.

Sorry the grow let you down!  I know you were all hoping these things would fruit in a less than stellar environment.  I suspect if I had picked a strain that was more apt to fruit in a "chamber" like environment, that it would have worked just fine.


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He's not the sharpest cookie in the cupboard.  What?

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InvisibleGrey
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21345334 - 02/28/15 06:01 PM (9 years, 10 months ago)

Your good man.  If you were doing the grow in optimal conditions and it failed, then maybe it would have been a bummer.  You made it that far which is still impressive. There's plenty of people state side that have all the resources necessary and still fail. Keep it up!


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If you don't have a plan of your own, you'll become a part of somebody else's.

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OfflinePsilicon
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Re: Freedom Fungus - First time grow needs opinions [Re: Grey]
    #21347415 - 03/01/15 09:44 AM (9 years, 10 months ago)

I think part of the reason you're able to pull off agar in open air and without sterilizing is because you're doing it in a desert, which will have fewer CFUs in the air.


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Offliner00tuuu123
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21347524 - 03/01/15 10:03 AM (9 years, 10 months ago)

Quote:

hoffzl01 said:
not blowed up!  Internet here has been pretty crappy.  So I failed at producing fruit.  I had to throw away the last of the mycellium a couple days ago.  However, things I learned.....

- You can do agar work without laboratory grade sterilization.  I made 20 different plates that myc successfully started crossing...and only lost 3 to contams after a two week period.  All using a microwave and no pressure cooker or even a still air box.  That is very intriguing.

- I believe Oysters need a VERY large amount of Oxygen (fresh air exchange)...and they do not enjoy a "tub" or "covered" setup.  I was forced into using this sub-optimal setup to keep the humidity up in this 10% RH environment...and I dont think it allows enough FAE to promote fruiting.  I got the one block to fruit once...but it quickly stalled out.

- Cardboard works....but I wont use it ever again.  While it's very easy to sterilize, it's just too slow for a substrate.  I tried a variety of "unique" substrates while I was here..and myc ran across most of them.  But cardboard was far and away the slowest.  It doesn't colonize evenly, it's awkward to deal with, requires shredding to quite small pieces, and doesn't hold moisture evenly.  I think there are "waste" products that would be a better fit, since cardboard can easily be recycled by standard methods.

- Obsessing over, and checking your grow every 15 minutes will not make it grow faster.

- Mycology is one of the most inexpensive and enjoyable hobbies I've seen

- Shroomery members are helpful, knowledgeable, and fun people

- I know exactly what I need to do when I get home in 6 weeks.

- Mushrooms will grow on just about anything.  Provide a carbohydrate, a sugar, some basic vitamins/minerals, and keep the ph right....and that stuff will grow.

Sorry the grow let you down!  I know you were all hoping these things would fruit in a less than stellar environment.  I suspect if I had picked a strain that was more apt to fruit in a "chamber" like environment, that it would have worked just fine.



Good to have you back man and not blowd up you learned a lot more than first time growers and probably more than those with 5-6 grows under their belts. :kaneclap: :seriousthumbsup:


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Offlinehoffzl01
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Registered: 01/22/15
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Re: Freedom Fungus - First time grow needs opinions [Re: Psilicon]
    #21352442 - 03/02/15 03:10 PM (9 years, 10 months ago)

Quote:

van der griegen said:
I think part of the reason you're able to pull off agar in open air and without sterilizing is because you're doing it in a desert, which will have fewer CFUs in the air.




I dont disagree with you. The lower particulate count probably had a lot to do with hit. I'm not advocating that anyone practice filling plates in open air as his/her standard.  I've already purchased a 30 quart pressure cooker for myself @home.  It's more just interesting that it can be done with extremely basic sterile care. 

The way people make it sound sometimes...if you dont use a pressure cooker your agar might grow into a small demon baby and eventually eat your face in the middle of the night.

Quote:

Good to have you back man and not blowd up you learned a lot more than first time growers and probably more than those with 5-6 grows under their belts.




Thanks buddy!  I did learn alot. It was time well spent.  I think I saved myself quite a few weeks of fumbling around at home when I finally get to do a real setup.  And it helped pass the time instead of staring at dirt for weeks.


--------------------
He's not the sharpest cookie in the cupboard.  What?

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