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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21232760 - 02/05/15 08:52 PM (9 years, 11 months ago)

So you like straight Agar -> LI -> Grain or Bulk?  LI being just mycellium water...Agar slurry if you want to call it that?

Sounds decent.  Agar to make sure it's clean, liquid to make it "go further" and colonize faster.  Straight to grain workers for a few days before bag substrate would probably work for me.

I'll compare that to the other closely related method of spawning agar out to grain or a cake and THEN making a liquid for further spreading. See what comes out on top in terms of speed and spawn material.


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He's not the sharpest cookie in the cupboard.  What?

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21232978 - 02/05/15 09:45 PM (9 years, 11 months ago)

I honestly haven't done it this way personally yet, but I've seen such great results with it and it seems so simple. Check out mudafuka's bottle tek thread.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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Offliner00tuuu123
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233142 - 02/05/15 10:40 PM (9 years, 11 months ago)

Mods please don't move this to edibles where it will languish in obscurity This guy knows his shit. As they say bullshit walks


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:kingcrankey: Please report me to a Mod for hurting your punk ass hippie feelings :flipthebird: And all time Champion thread killer.:thatsayes:

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21233153 - 02/05/15 10:46 PM (9 years, 11 months ago)

I've only been doing oysters for a month or so. Not much of my knowledge is practical, but there's a lot of good work done by others that I'm referring to. My only real experience with oysters is the growkit spawned to cardboard.

But yeah, I have a few threads on the edible board. Not much activity there. Mush cult gets so much more traffic. I don't make the rules, but I see no reason to not talk about edibles in mush cult on occasion.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233236 - 02/05/15 11:17 PM (9 years, 11 months ago)

Agreed.  Most of these techniques would apply to the basic variety of our more "magical" friends.  (From my reading and others research)  In fact, I will apply some of these techniques in a very magical way, when I'm not in such a public setting.  I didn't even realize it was in the wrong board.  But maybe the results will keep it here. 

This is the other half of that grow kit Numero. 



Crumbled in with shredded cardboard and various card stock boxes from parts that come here. (Cardboard water was supplemented) I'm trying to avoid opening it, but the temptation is almost irresistible.  I need to stop...so I can keep the carbon dioxide levels up while it colonizes.  It's starting to take over the cardboard very aggressively, and in a week or so, I'm going to slave it out to about 6 ziploc bags of cardboard. (Assuming it doesn't contaminate from me opening it...doh!)

Your bucket idea is the fruiting chamber.  The ziploc bags will have enough substrate that I can fit the resulting block into the bucket one at a time for fruiting.



These pictures are the same block using that bucket.  Less than 7 hours apart.  It's pretty ridiculous how fast these things grow once they take off.

0100 my time   

0800 my time   


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He's not the sharpest cookie in the cupboard.  What?

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21233256 - 02/05/15 11:22 PM (9 years, 11 months ago)

Nice. I'm printing this big one to grow out on agar. Then I'm gonna eat it. Probably gonna spawn the substrate to pasteurized sawdust bottles after that :cool:



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Edited by NumeroEno (02/05/15 11:23 PM)

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21233455 - 02/06/15 12:28 AM (9 years, 11 months ago)

you eat that thing man.  you eat it good.  The kit was a pretty cost effective way to get spores/mycellium/clones.  I'd do it that way again if I had to.  At least it's not a dead spore syringe that you have to wonder about for 2 weeks.


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He's not the sharpest cookie in the cupboard.  What?

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Offliner00tuuu123
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21233918 - 02/06/15 05:45 AM (9 years, 11 months ago)

hoffz you ever cook oysters?  That thing will turn to about the size of a 50 cent piece when its done cooking. :lol: but all in all you're on the right track. Good lookin out. (as the kids used to say) All hail the king of Ghetto teks. I wan't a print when you get that far. :thatsayes::manofapproval:


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:kingcrankey: Please report me to a Mod for hurting your punk ass hippie feelings :flipthebird: And all time Champion thread killer.:thatsayes:

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21235714 - 02/06/15 03:26 PM (9 years, 11 months ago)

You'll get a print!  I'm gonna put some of this desert sand in a baggie with it, and slap an American Flag on the package for yah.  haha


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He's not the sharpest cookie in the cupboard.  What?

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Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21244474 - 02/08/15 04:01 PM (9 years, 11 months ago)

*** UPDATE ***
2-9-2015

The grow kit is doing well.  It appears from the long stipe and small caps that I may have been a bit short on the FAE and possibly light...but I've done what I can.  It's tough riding that crazy fine line between fresh air and humidity when the RH of the air is 10%.  I used our magnifying bench light to give the 12/12 light as the sun here is a bit too intense.  It's only 3000K...so not quite high enough color temperature, but it's doing the job.  When I harvest, I will take multiple spore prints.  I am going to store some in water solution in vials, plate some, and LC some.  I'm not even going to attempt isolating until I get home.  But at least I will have a few vials of multispore I can start with.  No need to buy more.



The other half of the grow kit that I expanded to cardboard is colonizing ridiculous fast.  I'm going to give it 3-4 days to consolidate and then fruit it right in that plastic container by removing the lid and placing foil with multiple holes right on top the substrate pretty tightly.  These are just for eating.  Nom Noms.

I may try to use this substrate when I get home for bulk.  It is doing that well.  Since it was a kit, I dont know exactly what's in it..but doing a little research it appears to be hardwood sawdust and small amount of coffee grounds/wheat bran, with my added cardboard that was soaked in weak coffee and syrup solution.  Its worth further experiments, but seems to be a pretty basic recipe. I crumbled about 1/2 pint of colonized substrate into that only 3 days ago.



The Agar plates are coming along, although slowly.  If I do it again, I may add a slightly higher concentration of syrup 7.5 - 10%, or just use plain sugar at 5%.  I lost one plate out of four to mold, but I will accept 25% contam loss on plates that were not pressure cooked, and basically filled in open air.  I plan to put the plates into an LI (agar slurry) when they are fully colonized and inoculate cardboard.  No sectoring on the plates, but that's to be expected since it was started from already dikaryotic fully colonized mycellium. 



I was thinking about this last night, and I have to ask..but this question may be more suited for the advanced mycology board.  In the genetics of fungi, does taking a spore print from a fruit with certain desired traits increase the likelihood of re-producing those traits in one of the later isolates of that multispore?  As opposed to taking ANY random fruit from that block. 

Doesn't that fruit (even if it has certain desirable traits), still just have the same genetic make-up in the spores it produces, that all of the fruits from that same mycellium have?

I have seen people mention taking prints from fruits that had a trait they liked in an attempt to re-produce that trait (through isolation and lots of trial)...but I thought perhaps ANY fruit from that same mycellium (same block) would probably provide the same genetic code necessary to re-produce the desirable trait that appeared in that block. The fruit (no matter what genetic traits it presented) doesn't determine what genetics are in the spores it produces..right?

Just curious for when I get home and start isolating.  One fruit is much more "blue" than the rest and was thinking about why.  I'm not an expert...just a curious hobbyist.


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He's not the sharpest cookie in the cupboard.  What?

Edited by hoffzl01 (02/08/15 04:16 PM)

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21244953 - 02/08/15 05:52 PM (9 years, 11 months ago)

Mine isn't doing all that well ATM. I'm kind of using it as a gauge to dial in my greenhouse for when this batch of cubes I have going is ready to fruit. I also have a small bottle of cubes currently fruiting in there. I think I'm gonna try spawning this block to sawdust in a mycobag and dunk it really well before fruiting. This current flush is pretty disappointing, but I will at least get some prints from it.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
Offlinehoffzl01
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Registered: 01/22/15
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21244984 - 02/08/15 05:58 PM (9 years, 11 months ago)

Yeah, I noticed on mine when I broke it apart...that it was very very dry inside.  Even after the initial 12 hour soak that it called for.  The mycellium was so dense that the soak effectively did nothing.  I had to poke multiple holes with a fork all over the block and keep it very very moist.  I think you'll do just fine if you spawn it out and soak it well.  It seems to be a pretty healthy and aggressive strain.  It might be my "eating" strain when I get home.  I want to develop my own from wild samples...but if these taste good I'll keep them going.

I dont think this strain will get very big though.  One of the caps is already starting to go concave and it's only about an inch across.  AGain, could be FAE or humidity.  that one is getting printed as soon as it goes flat.


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He's not the sharpest cookie in the cupboard.  What?

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245011 - 02/08/15 06:06 PM (9 years, 11 months ago)

They can get pretty big. Here's a pic of the first flush I got out of the kit. I didn't dunk it this time and I think that's why this flush is pretty disappointing. I think it's also been expanded a whole bunch which is why I want to start some prints on agar. The one clone I took looked great but some trich snuck in and the grain jar went green.



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
Offlinehoffzl01
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Registered: 01/22/15
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245049 - 02/08/15 06:14 PM (9 years, 11 months ago)

Damnnnn...nice looking fruits.  I think mine is stalling out.  But I'll be patient and let it go for a few days.  I dont think they like growing off the top like I have it set up.  I've read that oysters dont take well to horizontal fruiting.  So strange that they can tell.


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He's not the sharpest cookie in the cupboard.  What?

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245075 - 02/08/15 06:22 PM (9 years, 11 months ago)

Yeah those were tasty. I wish I had printed one of them! I have a lot of king oysters going right now too, but I'm far from done with these. Next time I spawn them I'm gonna give the bag at least a week to consolidate and also do a 24 hour dunk. I'm really excited to do some multispore grows with these, though.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
Offlinehoffzl01
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245168 - 02/08/15 06:42 PM (9 years, 11 months ago)

I really like the deep blue color when they pin and young fruits.  It's aesthetically pleasing.  I'm excited as well to play around with these multispores.  I'm just excited in general that my first adventure after months of reading is working out.  Just 8 weeks until I go home and set up my man shroom cave.


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He's not the sharpest cookie in the cupboard.  What?

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Offliner00tuuu123
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Re: Freedom Fungus - First time grow needs opinions [Re: hoffzl01]
    #21245335 - 02/08/15 07:40 PM (9 years, 11 months ago)

Quote:

hoffzl01 said:
I really like the deep blue color when they pin and young fruits.  It's aesthetically pleasing.  I'm excited as well to play around with these multispores.  I'm just excited in general that my first adventure after months of reading is working out.  Just 8 weeks until I go home and set up my man shroom cave.



  you've done well Getting fruits to grow in a most inhospitable environment. Keep making prints and agar plates! Those have to be some hardy oysters. Don't know what area of the country you will be coming to but damn sure better than the desert. :thumbup: Oh and Imma hold you to that print when the weather breaks in a few weeks I might have some wild elm oyster prints to trade plus some really good other prints.


--------------------
:kingcrankey: Please report me to a Mod for hurting your punk ass hippie feelings :flipthebird: And all time Champion thread killer.:thatsayes:

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21245409 - 02/08/15 07:56 PM (9 years, 11 months ago)

Well I think I screwed this current flush up by not dunking beforehand. I decided to pull the few fruits off and dunk it because it was so dry. I had a hard time keeping the block submerged under a gallon jug of water, and there were air bubbles coming out of the sub for a while :facepalm:

I'm gonna put it back into fruiting conditions in about a week, because I want to give it more time to eat the sub. Gonna dunk it again before fruiting too.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
Offliner00tuuu123
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Re: Freedom Fungus - First time grow needs opinions [Re: NumeroEno]
    #21245535 - 02/08/15 08:24 PM (9 years, 11 months ago)

Quote:

NumeroEno said:
Well I think I screwed this current flush up by not dunking beforehand. I decided to pull the few fruits off and dunk it because it was so dry. I had a hard time keeping the block submerged under a gallon jug of water, and there were air bubbles coming out of the sub for a while :facepalm:

I'm gonna put it back into fruiting conditions in about a week, because I want to give it more time to eat the sub. Gonna dunk it again before fruiting too.



You don't need to do all that with oysters just watter it with a watering can and wait a few days and it'll flush again till there's no more nutrient to feed it.


--------------------
:kingcrankey: Please report me to a Mod for hurting your punk ass hippie feelings :flipthebird: And all time Champion thread killer.:thatsayes:

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InvisibleNumeroEno
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Re: Freedom Fungus - First time grow needs opinions [Re: r00tuuu123]
    #21245558 - 02/08/15 08:32 PM (9 years, 11 months ago)

Well I can't really pour a watering can all over my greenhouse so that's why I'm dunking it.

You're right though. I guess there's no real reason to put in back into colonizing conditions right now. I'm just gonna let it soak overnight and put it back into fruiting conditions.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
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