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OfflineDorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
Adding coffee to spore syringes exp.
    #21151135 - 01/20/15 05:09 PM (9 years, 3 months ago)

A few weeks ago I had a used syringe from a recent inoculation project, I noticed spores still stuck around the sides. My idea was to run water through the coffee pot for a somewhat distillation process and use the water to make another spore syringe. A few coffee grounds got mixed in with the spores, I believe colonization was a bit quicker than the first  round of inoculation with out the coffee grounds. Ik its been used in substrates for a bit of an added pick me up but risk increasing contam. I want to try more tests but what do other people think.

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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 7 months
Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
    #21151149 - 01/20/15 05:12 PM (9 years, 3 months ago)

Just push the syringe all the way down? I wouldn't trust that method unless you plan to put it to agar. If you're gonna do it use distilled water in a pot.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

Edited by taGyo (01/20/15 05:12 PM)

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OfflineDorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
Re: Adding coffee to spore syringes exp. [Re: taGyo]
    #21151176 - 01/20/15 05:20 PM (9 years, 3 months ago)

I wouldn't mind trying agar but lack equipment, why would it be safer with agar? There seems to alway be some left overs on the plunger or trapped in the needle.
Ur pyramid rack is dank btw

Edited by Dorfnob (01/20/15 05:21 PM)

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 1 hour
Trusted Cultivator
Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
    #21151179 - 01/20/15 05:21 PM (9 years, 3 months ago)

Quote:

Dorfnob said:
My idea was to run water through the coffee pot for a somewhat distillation process



No even close to distillation.....all a coffee pot does is heat the water.....you can do that in a pot on a stove....

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OfflineDorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
Re: Adding coffee to spore syringes exp. [Re: PussyFart]
    #21151215 - 01/20/15 05:28 PM (9 years, 3 months ago)

Your very right I should've known how a coffee machine works. That  helps explain the contam issue

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Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
    #21151236 - 01/20/15 05:34 PM (9 years, 3 months ago)

Distilled water
Astro Glide
syringes
PC
Gloves
SAR OR SAB OR FH
shower
rubbing alcohol
Paper towels
Torch lighter
Foil
mason jar
inoculation loop
spores


----
These are the tools I need to make syringes.

:awetrippie:

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
    #21152025 - 01/20/15 08:14 PM (9 years, 3 months ago)

Quote:

Dorfnob said:
Your very right I should've known how a coffee machine works. That  helps explain the contam issue




Not many people watch nerdy videos on the internet about how the heating element of a coffee pot forces water through a bubbler valve.  Don't beat yourself up over it.  :wink:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 1 hour
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Re: Adding coffee to spore syringes exp. [Re: Psilicon]
    #21152043 - 01/20/15 08:17 PM (9 years, 3 months ago)

I didn't have to watch a video to know that a coffee pot heats water.....you put in cold water and you get out hot water....Is that distilled? no  :smirk:


Edited by PussyFart (01/20/15 08:17 PM)

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: Adding coffee to spore syringes exp. [Re: PussyFart]
    #21152080 - 01/20/15 08:22 PM (9 years, 3 months ago)

Where did you learn that still don't heat water?  :grin:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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