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Dorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
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Adding coffee to spore syringes exp.
#21151135 - 01/20/15 05:09 PM (9 years, 3 months ago) |
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A few weeks ago I had a used syringe from a recent inoculation project, I noticed spores still stuck around the sides. My idea was to run water through the coffee pot for a somewhat distillation process and use the water to make another spore syringe. A few coffee grounds got mixed in with the spores, I believe colonization was a bit quicker than the first round of inoculation with out the coffee grounds. Ik its been used in substrates for a bit of an added pick me up but risk increasing contam. I want to try more tests but what do other people think.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 7 months
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Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
#21151149 - 01/20/15 05:12 PM (9 years, 3 months ago) |
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Just push the syringe all the way down? I wouldn't trust that method unless you plan to put it to agar. If you're gonna do it use distilled water in a pot.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (01/20/15 05:12 PM)
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Dorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
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Re: Adding coffee to spore syringes exp. [Re: taGyo]
#21151176 - 01/20/15 05:20 PM (9 years, 3 months ago) |
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I wouldn't mind trying agar but lack equipment, why would it be safer with agar? There seems to alway be some left overs on the plunger or trapped in the needle. Ur pyramid rack is dank btw
Edited by Dorfnob (01/20/15 05:21 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 1 hour
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Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
#21151179 - 01/20/15 05:21 PM (9 years, 3 months ago) |
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Quote:
Dorfnob said: My idea was to run water through the coffee pot for a somewhat distillation process
No even close to distillation.....all a coffee pot does is heat the water.....you can do that in a pot on a stove....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Dorfnob
J.U.I.C.E.


Registered: 07/28/12
Posts: 737
Last seen: 1 day, 13 hours
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Re: Adding coffee to spore syringes exp. [Re: PussyFart]
#21151215 - 01/20/15 05:28 PM (9 years, 3 months ago) |
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Your very right I should've known how a coffee machine works. That helps explain the contam issue
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
#21151236 - 01/20/15 05:34 PM (9 years, 3 months ago) |
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Distilled water Astro Glide syringes PC Gloves SAR OR SAB OR FH shower rubbing alcohol Paper towels Torch lighter Foil mason jar inoculation loop spores
---- These are the tools I need to make syringes.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
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Re: Adding coffee to spore syringes exp. [Re: Dorfnob]
#21152025 - 01/20/15 08:14 PM (9 years, 3 months ago) |
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Quote:
Dorfnob said: Your very right I should've known how a coffee machine works. That helps explain the contam issue
Not many people watch nerdy videos on the internet about how the heating element of a coffee pot forces water through a bubbler valve. Don't beat yourself up over it.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 1 hour
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Re: Adding coffee to spore syringes exp. [Re: Psilicon]
#21152043 - 01/20/15 08:17 PM (9 years, 3 months ago) |
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I didn't have to watch a video to know that a coffee pot heats water.....you put in cold water and you get out hot water....Is that distilled? no 
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (01/20/15 08:17 PM)
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
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Re: Adding coffee to spore syringes exp. [Re: PussyFart]
#21152080 - 01/20/15 08:22 PM (9 years, 3 months ago) |
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Where did you learn that still don't heat water?
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