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Offlinecosmo4200
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Registered: 11/06/14
Posts: 21
Last seen: 9 years, 1 month
need a little advice
    #21144274 - 01/19/15 01:56 PM (9 years, 3 months ago)

Got 10 pre sterilized 1 pound bags of rye with injection port I knocked em up and now on some of the rye grains there is a bluish greenish hue to them there is also white grains whats goin on I am sure of my sterile techniques could it be bruising bcuz I have played with them a bit I know thats not advised but Im just trying to figure out if it a contam or not I can post pics but someone will have to show me how thanks




Edited by cosmo4200 (01/19/15 01:59 PM)

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OfflineCalifornian
I'm a teapot

Registered: 12/13/14
Posts: 81
Last seen: 3 months, 6 days
Re: need a little advice [Re: cosmo4200]
    #21144334 - 01/19/15 02:04 PM (9 years, 3 months ago)

You have a contamination. Most likely Aspergillus or Penicillium. I forget which one is more common or why you would get one over the other, but it is not mushroom mycelium. Sorry man.

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Offlinecosmo4200
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Registered: 11/06/14
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Last seen: 9 years, 1 month
Re: need a little advice [Re: Californian]
    #21144349 - 01/19/15 02:07 PM (9 years, 3 months ago)

Can the mycelium in the bag fight it off or is it a lost cause

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Offlinecosmo4200
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Registered: 11/06/14
Posts: 21
Last seen: 9 years, 1 month
Re: need a little advice [Re: cosmo4200]
    #21144356 - 01/19/15 02:08 PM (9 years, 3 months ago)

It is ecuador and I put 5 cc in each bag

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OfflineAdrenalien
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Registered: 11/12/12
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Last seen: 7 years, 4 months
Re: need a little advice [Re: cosmo4200]
    #21144397 - 01/19/15 02:15 PM (9 years, 3 months ago)

I would consider it lost. I have lost jars to similar green contaminations before. The mycelium couldn't fight it off, and eventually the contam overtook the jar.

I'd scrap it, and try again. I know that's no fun to hear. Sorry man.

Wait for others to chime in though. Maybe they know something I don't.

Also, this thread is in the wrong place. It might become recategorized into the Contamination Forum or in Mushroom Cultivation.

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Offlinecosmo4200
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Registered: 11/06/14
Posts: 21
Last seen: 9 years, 1 month
Re: need a little advice [Re: Adrenalien]
    #21144417 - 01/19/15 02:20 PM (9 years, 3 months ago)

Kk thanks for the replys they have been isolated for now till I get further info and im a neby so by all means place this thread where it belongs

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Offlineknomadic_niki
A mile high
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Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
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Re: need a little advice [Re: cosmo4200]
    #21145245 - 01/19/15 04:55 PM (9 years, 3 months ago)

if you bought pre-sterilized bags with self sealing injection port and a spore syringe, you don't need sterile technique. the contam came from one or the other and it sounds like the company that sent them to you should send you another batch


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InvisibleJuiceh
Dabbing All Day
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Registered: 09/25/12
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Re: need a little advice [Re: knomadic_niki]
    #21147038 - 01/19/15 10:11 PM (9 years, 3 months ago)

Quote:

knomadic_niki said:
if you bought pre-sterilized bags with self sealing injection port and a spore syringe, you don't need sterile technique.


I disagree. You should still torch the needle between inoculations and be reasonably clean.

I bet the spore syringe is probably the culprit tho. Take a few drops of the spore solution to some agar plates and see if it's clean. It's possible you contaminated it during inoculation. But if your sterile technique was good the only contams that show up on the agar probably were in the syringe when you got it.


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Offlineknomadic_niki
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Re: need a little advice [Re: Juiceh]
    #21147498 - 01/20/15 12:07 AM (9 years, 3 months ago)

oh yea, you're right, juice. i didnt think of the needle as a vector. its been a long time since i've grown actives


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OfflineP. tetras
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Registered: 12/30/14
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Last seen: 9 years, 3 months
Re: need a little advice [Re: knomadic_niki]
    #21147537 - 01/20/15 12:17 AM (9 years, 3 months ago)

Why is there any need to argue on how many time we need to sterilize things, it's like momma always said, "better safe than sorry". With this in mind why not take the extra 1-2 minutes to make sure everything is properly sterilized.

:cool: just saying

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InvisibleJuiceh
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Re: need a little advice [Re: P. tetras]
    #21148816 - 01/20/15 09:58 AM (9 years, 3 months ago)

Who's arguing?


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Offlineknomadic_niki
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Re: need a little advice [Re: Juiceh]
    #21149051 - 01/20/15 10:53 AM (9 years, 3 months ago)

no argument here. i was just saying that if the sterilization work is already done for the guy and all he needs to do is stick a needle through a port, there's not much sterile technique needed. except, as juice pointed out, sterilizing the needle. i agree with him

the purpose of my statement, though, is that it is most probable that the contamination came from the syringe or the pre-sterilized grain.


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Edited by knomadic_niki (01/20/15 10:54 AM)

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OfflineBoletus Sequoia
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Registered: 09/30/14
Posts: 181
Loc: So. Oregon
Last seen: 6 years, 5 months
Re: need a little advice [Re: knomadic_niki]
    #21149385 - 01/20/15 11:45 AM (9 years, 3 months ago)

If the OP used a sterile needle and wiped the injection port with an alcohol swab I think it's safe to say it the vendor's bad

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Offlinecosmo4200
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Registered: 11/06/14
Posts: 21
Last seen: 9 years, 1 month
Re: need a little advice [Re: Boletus Sequoia]
    #21153161 - 01/20/15 11:20 PM (9 years, 3 months ago)

Thanks everyone for there responses

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Offlinecosmo4200
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Registered: 11/06/14
Posts: 21
Last seen: 9 years, 1 month
Re: need a little advice [Re: cosmo4200]
    #21158279 - 01/21/15 04:19 PM (9 years, 3 months ago)

A little update one of my bags that had a spot of the penacillium has bounced back there is agressive mycelium growth and I cannot fint the mold nemore

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